Activities and biological evaluations of probiotic bacteria strains

Afify, A. M.; Romeilah, Ramy M.; Sultan, Shaimaa I.M.; Hussein, M. M.

doi:10.7813/2075-4124.2012/4-6/a.19

Cited by 25 publications

(5 citation statements)

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“…In contrast, A fy and his colleagues have found an important DPPH antioxidant activity reaching a value of 97.75% for the cell free extract of Propionibacterium freudenreichii ssp. Shermanii (ATCC 1907) [33], which is higher according to our results.…”

Section: Discussionsupporting

confidence: 68%

Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Hamdaoui,

Azghar,

Benkirane

et al. 2023

Preprint

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Background Streptococcus thermophilus were considered one of probiotic bacterial strain; found in milk, cheese, and yogurt, that providing health benefits to humans. Due to their probiotic potentiality, S. thermophilus can be very helpful in replacing chemicals preservatives. This study came out to investigate the probiotic activity of ten S. thermophilus isolates obtained from raw cow milk in eastern Morocco against multidrug-resistant bacteria (MDR) bacteria: Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC: 25922 and 35218). Besides their antioxidant potential, bile salts tolerance as well as their antibiotic susceptibility, were evaluated Result The results showed remarkable in vitro inhibitory ability of the studied S. thermophilus isolates against the tested multidrug-resistant bacteria, as reported for S. thermophilus CFS and supernatant against Staphylococcus aureus (ATCC29213), confirming their potential as antibacterial agents. The mixtures of NaOH, catalase and proteinase K showed no inhibition zones against all tested multidrug-resistant bacteria; suggesting the main antibacterial effect of all isolates based on acids, hydrogen peroxide and/or proteins. For the antioxidant activity, the DPPH inhibition values varied between 18.97% and 44.44%, for the bacterial isolate ST9 and ST5, respectively. Notably, S. thermophilus isolates were able to survive up to 6h of incubation with a concentration of 0.1% of bile salt at 37°C. Thus, ST5 could be considered as the most promising bacterial isolate in terms of antioxidant activity. All isolates of S. thermophilus exhibited sensibility to all tested antibiotics except for rifampicin and trimethoprim-sulfamethoxazole compared to the standards proposed by the European committee on antimicrobial susceptibility testing. Conclusions The tested isolates were exhibited excellent antibacterial and potential antioxidant activities. In addition to that, these isolates were reported to resist rifampicin and trimethoprim-sulfamethoxazole antibiotics. These findings presage the use of S. thermophilus isolates as good probiotics.

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Section: Discussionsupporting

confidence: 68%

Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Hamdaoui,

Azghar,

Benkirane

et al. 2023

Preprint

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“…Free radical scavenging activity of cell extract was estimated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), that is a stable free radical and decolorized when acquire an electron (Afify et al [2012]). The bacterial cells grown to OD 600nm = 10 in BHI medium were harvested (5,000 rpm for 30 min at 4°C) and disrupted by Mini-BeadBeater16 (BioSpec, Bartlesville, OK, USA) to prepare the cell free extract.…”

Section: Methodsmentioning

confidence: 99%

Artificial oxidative stress-tolerant Corynebacterium glutamicum

Lee

Seo

et al. 2014

AMB Expr

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We have reported a transcription profile of an adapted Corynebacterium glutamicum that showed enhanced oxidative stress resistance. To construct an artificial oxidative stress-resistant strain, gene clusters in the β-ketoadipate pathway, which were up-regulated in the adapted strain, were artificially expressed in the wild-type C. glutamicum. The wild-type strain was unable to grow under 2 mM H2O2 containing minimal medium, while the strains expressing pca gene clusters restored growth under the same medium, and the pcaHGBC expression showed the most significant effect among the gene clusters. The expressions of pca gene clusters also enabled the wild-type to increase its resistance against oxidative stressors, such as diamide and cumene hydroperoxide, as well as H2O2. The oxidative stress tolerance of the strain was correlated to the reactive oxygen species (ROS)-scavenging activity of the cell extract. The reason for the enhanced oxidative stress-resistance of C. glutamicum and its applications on the synthetic strain development are discussed.

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“…By collaborating with selenium-dependent GSH peroxidase, GSH eliminates radicals such as hydroxyl radicals and hydrogen peroxides. Additionally, probiotics stimulate the production of certain antioxidant biomolecules, including exopolysaccharides [ 35 ]. Çakmakçı et al [ 36 ] revealed that yogurt produced with classic culture + Lactobacillus acidophilus had higher antioxidant properties than that of control made using classic culture.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the Ayran Made with Commercial Probiotic Cultures for Fatty Acids, Cholesterol, Folic Acid Levels, and Anti-Oxidative Potential

Shalabi

Hassan

Ismail

et al. 2023

Probiotics & Antimicro. Prot.

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Ayran is a salted drinkable fermented milk food which consumed in many countries around the world. In this study, some chemical parameters were determined to evaluate the healthy properties of ayran prepared using various commercial probiotic cultures. Four treatments of ayran were made from cow’s milk and using classic yogurt culture (L. delbrueckii subsp. bulgaricus and Streptococcus thermophilus) [T1], ABT-5 culture (L. acidophilus, Bifidobacterium and S. thermophilus) [T2], exopolysaccharide producing culture (EPS-producing, L. delbrueckii subsp. bulgaricus and S. thermophilus) [T3], and EPS-producing culture + Bifidobacterium animalis subsp. lactis BB12 (mixture culture) [T4]. Treatment 1 had the highest acidity, acetaldehyde, and diacetyl values. Using probiotic [T2] or mixture cultures [T4] reduced saturated fatty acids by 1.97% and increased monounsaturated and polyunsaturated fatty acids of ayran by 4.94 and 5.72%, respectively. Also, the levels of oleic acid (omega-9), linoleic acid (omega-6), and α-linolenic acid (omega-3) increased in ayran produced using probiotic or mixture cultures. Sample T4 was highly richer in the value of antioxidant activity (27.62%) and folic acid (0.1566 mg/100 g) whereas possessed the lowest cholesterol amount (8.983 mg/100 g). Mixture culture (EPS-producing culture + Bifidobacterium animalis subsp. lactis BB12) is a good starter to improve the healthy and nutritional characteristics of bio-ayran.

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Activities and biological evaluations of probiotic bacteria strains

Cited by 25 publications

References 0 publications

Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Artificial oxidative stress-tolerant Corynebacterium glutamicum

Characterization of the Ayran Made with Commercial Probiotic Cultures for Fatty Acids, Cholesterol, Folic Acid Levels, and Anti-Oxidative Potential

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