Activities Analysis and Polymorphisms Identification of GPIHBP1 Promoter Region in Porcine

Regulatory Mechanism of Transcription Factor AhHsf Modulates AhHsp70 Transcriptional Expression Enhancing Heat Tolerance in Agasicles hygrophila (Coleoptera: Chrysomelidae)

“…In contrast, other studies have found that some promoters contain CpG islands, which are believed to play an important role in the epigenetic regulation of these genes and are generically equipped to influence local chromatin structure and assist in the regulation of gene activity [ 50 , 51 , 52 , 53 ]. Moreover, promoters serve as key cis -acting elements that regulate gene expression, and it is generally believed that the cis- and trans -regulatory machinery in promoter regions are basic requirements for gene expression [ 54 ]. In 2020, Jia et al identified six AhHsp70 s ( hsp70-1 , hsp70-2 , hsp70-3 , hsp70-4 , hsp70-5 , hsp70-6 ) in A. hygrophila [ 55 ]; all six Hsp70s of A. hygrophila had a non-organellar consensus motif RARFEEL [ 56 ], and the C-terminal sequences included the EEVD motif for cytoplasmic localization.…”

“…Promoter deletion analysis is one of the primary and most widely used techniques employed to determine whether they are cis -acting elements or specific transcription factor binding sites within a promoter that are primarily responsible for the transcriptional regulation of a particular gene [ 49 , 57 ]. Luciferase (firefly and Renilla luciferases) genes have been used extensively as reporter genes because of their sensitivity and efficiency [ 18 , 54 , 58 ]. For example, Apriana et al demonstrated the root-specific expression of an alkenal reductase gene ( OsAER1 ) in Oryza sativa , based on the deletion analysis of the OsAER1 gene promoter [ 59 ].…”