Activin A-mediated epithelial de-differentiation contributes to injury repair in an in vitro gastrointestinal reflux model

Roudebush, Cedric; Catala-Valentin, Alma; Andl, Thomas; Bras, Grégoire F. Le; Andl, Claudia D.

doi:10.1016/j.cyto.2019.154782

Cited by 10 publications

(9 citation statements)

References 75 publications

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“…Esophageal mucosal lesions that develop in response to noxious stimuli induce protective responses, such as mucosal repair and tissue restitution, which are mediated by various physiological events, such as cell motility, cell–cell adhesion, keratinocyte dedifferentiation, and angiogenesis. 38 The processes involved in wound healing and tissue development were predicted in the enrichment analysis based on our sequencing data, providing evidence for the theory that although macroscopically intact, the esophageal mucosal integrity is impaired in NERD. 7 Instead of cell death and apoptosis, inflammation-regulated processes were found to be enriched in this study, reinforcing the concept that the pathogenesis of GERD is initiated by cytokine-driven mucosal damage, rather than the death of surface cells-triggered inflammatory responses.…”

Section: Discussionsupporting

confidence: 62%

Identification of Unique Transcriptomic Signatures and Hub Genes Through RNA Sequencing and Integrated WGCNA and PPI Network Analysis in Nonerosive Reflux Disease

Zhao¹,

Ma²,

Zou³

2021

JIR

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Section: Discussionsupporting

confidence: 62%

Identification of Unique Transcriptomic Signatures and Hub Genes Through RNA Sequencing and Integrated WGCNA and PPI Network Analysis in Nonerosive Reflux Disease

Zhao¹,

Ma²,

Zou³

2021

JIR

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“…Besides, the amino acid sequence of activin A in mice shared 100% identity to that in humans (Schwall et al, 1988). Activin A is expressed in a variety of tissues and cells, and exerts a critical regulatory function in inflammation, immunity, tumor, tissue repair and fibrosis (Morianos et al, 2019;Roudebush et al, 2019;Zhang et al, 2019). However, it remains unclear whether activin A affects adhesion and migration of fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices

Jiang

Kong

et al. 2021

Front. Cell Dev. Biol.

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Activin A, a member of the transforming growth factor-beta (TGF-β) superfamily, contributes to tissue healing and fibrosis. As the innate tissue cells, fibroblasts also play an important role in wound healing and fibrosis. Herein, this study was aimed to investigate how activin A exhibited regulatory effects on adhesion and migration of fibroblasts. We found that activin A induced the migration of fibroblast cell line L929 cells in transwell chamber and microfluidic device. Activin A also promoted L929 cells adhesion, but did not affect L929 cells viability or proliferation. In addition, activin A induced α-SMA expression and TGF-β1 release, which were factors closely related to tissue fibrosis, but had no effect on IL-6 production, a pro-inflammatory cytokine. Furthermore, activin A elevated calcium levels in L929 cells and increased p-ERK protein levels. Activin A-induced migration of L929 cells was attenuated by ERK inhibitor FR180204. To conclude, these data indicated that activin A as a novel chemokine induced the chemotactic migration of L929 cells via ERK signaling and possessed the pro-fibrosis role. These findings provide a new insight into understanding of activin A in tissue fibrosis.

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“…Tissue slides were treated as previously described 31 . Primary antibodies against KRT8 (1:200; Santa Cruz Biotechnology, clone Ks8-7) and rabbit ATP13A3 (1:400) were applied in 1X PBS/1%BSA solution and incubated overnight at room temperature in a humidified chamber.…”

Section: Methodsmentioning

confidence: 99%

ATP13A3 facilitates polyamine transport in human pancreatic cancer cells

Sekhar

Andl

Phanstiel

2022

Sci Rep

Self Cite

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The purpose of this study is to provide an increased understanding of the molecular mechanisms responsible for mammalian polyamine transport, a process that has been a long-standing ‘black box’ for the polyamine field. Here, we describe how ATP13A3, a P-type ATPase, functions as a polyamine transporter in response to different polyamine stimuli and polyamine-targeted therapies in highly proliferating pancreatic cancer cells. We assessed the expression, cellular localization and the response of the human ATP13A3 protein to polyamine treatments in different pancreatic cancer cell lines using Western blot and immunofluorescence microscopy. Using CRISPR mutagenesis and radiolabeled polyamine uptake assays, we investigated the role of ATP13A3 protein in polyamine transport. Highly metastatic cancer cells with high polyamine import express higher levels of the full-length ATP13A3 compared to cells with slow proliferation and low import activity. Highlighting its role in polyamine trafficking, the localization of ATP13A3 is altered in the presence of polyamine stimuli and polyamine-targeted therapies in these cells. Using CRISPR mutagenesis, we demonstrate that the first membrane-associated domain of this protein is critical and indispensable for its function as a spermidine and spermine transporter in cells. Further analysis of existing databases revealed that pancreatic cancer patients with high expression of ATP13A3 have decreased overall survival consistent with the role of intracellular polyamines in supporting tumor growth. Our studies shed light on the mysterious polyamine transport process in human cells and clearly establishes ATP13A3 as an intrinsic component of the spermidine and spermine transport system in humans.

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Activin A-mediated epithelial de-differentiation contributes to injury repair in an in vitro gastrointestinal reflux model

Cited by 10 publications

References 75 publications

Identification of Unique Transcriptomic Signatures and Hub Genes Through RNA Sequencing and Integrated WGCNA and PPI Network Analysis in Nonerosive Reflux Disease

Identification of Unique Transcriptomic Signatures and Hub Genes Through RNA Sequencing and Integrated WGCNA and PPI Network Analysis in Nonerosive Reflux Disease

Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices

ATP13A3 facilitates polyamine transport in human pancreatic cancer cells

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