Abstract:Surface-enhanced Raman scattering (SERS) technique is becoming highly popular for multiplex biosensing due to the ‘fingerprint’ Raman spectra from every molecule. As a proof-of-concept, we demonstrated the actively targeted multiplex in vitro and in vivo detection of three intrinsic cancer biomarkers - EGFR, CD44 and TGFβRII in a breast cancer model using three multiplexing capable, biocompatible SERS nanoparticles/nanotags. Intra-tumorally injected antibody conjugated nanotags specifically targeting the three… Show more
“…SERS is a plasmatic effect [1,23]; the term is derived from plasmon. When conductive electrons of metals (mostly noble metals), due to impaction of an electromagnetic beam (e.g.…”
Section: Principles Of Raman Spectroscopymentioning
confidence: 99%
“…SERS nano-tags also have many significant advantages over fluorescence based NPs like quantum dots, e.g. (i) multiplex detection capability due to spectral fingerprinting; (ii) not being vulnerable to photo-bleaching; and (iii) low cytotoxicity due to the usage of AuNPs [1]. 10.1080/20022727.2017.1373551-F0003Figure 3.Schematic illustration of formation of sandwich structure and SERS HOT spot with both SERS tags (anti-CEA/4-ATP/Fe 3 O 4 -Au NPs) and SERS – active substrate [41].…”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…According to the GLOBOCAN database in 2012, there were an estimated 14.1 million new cases of cancer in the world[s8] , that lung, breast and bowel cancers (including anus), were most prevalent cancer cases. Early detection, with all its proper subsets, is essential for effective and perfect treatment of cancer [1,2]. Advances in medical science show promise in the treatment of cancer.…”
In vitro detection technique Raman spectroscopy (Rs), in one number times another Rs based expert ways of art and so on, are useful instruments for cancer discovery. top gave greater value to Raman spectroscopy sers is a relatively new careful way for in vitro and in vivo discovery that takes away bad points of simple Raman spectroscopy (Rs). Raman spectroscopy (RS) and in particular, multiple RS-based techniques are useful for cancer detection. Surface enhanced Raman spectroscopy (SERS) is a relatively new method for both in vitro and in vivo detection, which eliminates the drawbacks of simple RS. Using nanoparticles has elevated the sensitivity and specificity of SERS. SERS has the potential to increase sensitivity, specificity and spatial resolution in cancer detection, especially in cooperation with other diagnostic imaging tools such as magnetic resonance imaging (MRI) and PET-scan polyethylene terephthalate. Developing a hand held instrument for detecting cancer or other illnesses may also be feasible by using SERS. Frequently, novel nanoparticles are used in SERS. With a focus on nanoparticle utilization, we review the benefits of RS in cancer detection and related biomarkers. With a focus on nanoparticles utilizations, the benefits of RS in cancer detection and related biomarkers were reviewed. In addition, Raman applications to detect some of prevalent were discussed. Also more investigated cancers such as breast and colorectal cancer, multiple nanostructures and their possible special biomarkers, especially as SERS nano-tag have been reviewed. The main purpose of this article is introducing of most popular nanotechnological approaches in cancer detection by using Raman techniques. Moreover, have been caught up on detection and reviewed some of the most prevalent and also more investigated cancers such as breast, colorectal cancer, multiple intriguing nanostructures, especially as SERS nano-tag, special cancer biomarkers and related approaches. The main purpose of this article is to introduce the most popular nanotechnological approaches in cancer detection by using Raman techniques.
“…SERS is a plasmatic effect [1,23]; the term is derived from plasmon. When conductive electrons of metals (mostly noble metals), due to impaction of an electromagnetic beam (e.g.…”
Section: Principles Of Raman Spectroscopymentioning
confidence: 99%
“…SERS nano-tags also have many significant advantages over fluorescence based NPs like quantum dots, e.g. (i) multiplex detection capability due to spectral fingerprinting; (ii) not being vulnerable to photo-bleaching; and (iii) low cytotoxicity due to the usage of AuNPs [1]. 10.1080/20022727.2017.1373551-F0003Figure 3.Schematic illustration of formation of sandwich structure and SERS HOT spot with both SERS tags (anti-CEA/4-ATP/Fe 3 O 4 -Au NPs) and SERS – active substrate [41].…”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…According to the GLOBOCAN database in 2012, there were an estimated 14.1 million new cases of cancer in the world[s8] , that lung, breast and bowel cancers (including anus), were most prevalent cancer cases. Early detection, with all its proper subsets, is essential for effective and perfect treatment of cancer [1,2]. Advances in medical science show promise in the treatment of cancer.…”
In vitro detection technique Raman spectroscopy (Rs), in one number times another Rs based expert ways of art and so on, are useful instruments for cancer discovery. top gave greater value to Raman spectroscopy sers is a relatively new careful way for in vitro and in vivo discovery that takes away bad points of simple Raman spectroscopy (Rs). Raman spectroscopy (RS) and in particular, multiple RS-based techniques are useful for cancer detection. Surface enhanced Raman spectroscopy (SERS) is a relatively new method for both in vitro and in vivo detection, which eliminates the drawbacks of simple RS. Using nanoparticles has elevated the sensitivity and specificity of SERS. SERS has the potential to increase sensitivity, specificity and spatial resolution in cancer detection, especially in cooperation with other diagnostic imaging tools such as magnetic resonance imaging (MRI) and PET-scan polyethylene terephthalate. Developing a hand held instrument for detecting cancer or other illnesses may also be feasible by using SERS. Frequently, novel nanoparticles are used in SERS. With a focus on nanoparticle utilization, we review the benefits of RS in cancer detection and related biomarkers. With a focus on nanoparticles utilizations, the benefits of RS in cancer detection and related biomarkers were reviewed. In addition, Raman applications to detect some of prevalent were discussed. Also more investigated cancers such as breast and colorectal cancer, multiple nanostructures and their possible special biomarkers, especially as SERS nano-tag have been reviewed. The main purpose of this article is introducing of most popular nanotechnological approaches in cancer detection by using Raman techniques. Moreover, have been caught up on detection and reviewed some of the most prevalent and also more investigated cancers such as breast, colorectal cancer, multiple intriguing nanostructures, especially as SERS nano-tag, special cancer biomarkers and related approaches. The main purpose of this article is to introduce the most popular nanotechnological approaches in cancer detection by using Raman techniques.
“…Apart from these various methods, scientists have invented numerous nanomaterials to differentiate different tumor biomarkers, such as cadmium, lead and copper alginate nanobeads [25], quantum dots [26], graphene oxide [27], silver-nanoparticle-enriched carbon nanotube [28], gold nanoparticles [29] and polystyrene microspheres [30]. Recently, surface-enhanced Raman scattering (SERS) based methods for multiple tumor biomarkers detection have got a lot of attentions because of its narrow 'fingerprint' Raman spectrum from analyte molecule, which enables the SERS to be utilized to recognize and quantify various targets from mixture [31]. With the development of various nanomaterials, SERS was utilized for detecting multiple cancer biomarkers successfully [32][33][34][35][36].…”
mentioning
confidence: 99%
“…Li et al designed an innovative SERS technique using branched DNA-gold nanoaggregates to detect MUC1mucin and nucleolin on breast cancer cell surface [34]. Through integrating SERS method with hollow core photonic crystal fiber, Dinish et al constructed a platform to detect multiple cancer biomarkers simultaneously with low sample volume [31]. Based on innovative ordered silver nanoshells silica photonic crystal bead, Li et al developed an SERS technique for CEA and AFP detection in a single run via a sandwich structure, which showed good sensitivity, high efficiency and low cross-reactivity [35].…”
Aim:Here, a multiplex surface-enhanced Raman scattering (SERS) based assay for simultaneous quantitation of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) was developed. Methods: SERS tags of nanostars and SERS substrates of nanobowl arrays were functionalized with labeling and capturing antibodies, respectively. In presence of antigens, SERS tags, antigens and SERS substrates formed sandwich structure. Results: The SERS-based technique showed a wide linear range from 0.5 to 100 ng/ml and detection limits were 0.41 and 0.35 ng/ml for CEA and AFP in phosphate-buffered saline buffer, respectively. Analysis results of clinical serum samples using this technique were similar to that shown in phosphatebuffered saline buffer. The LODs were 0.44 and 0.40 ng/ml for CEA and AFP, respectively. Conclusion: The precision and stability of this analysis technique were satisfactory, meanwhile, no obvious cross-reactivity could be found. What's more, it also suggested that this novel multiplex SERS-based technique could be a simple, specific, reliable, sensitive and multiplexed tool for important diagnostic and prognostic applications. Cancer is a latent fatal illness which kills millions of people worldwide, and the number of people dying from cancer continues to rise. If they were detected and cured earlier, many patients who are dying from cancer would have been saved [1,2]. In clinical diagnosis of cancer, detecting tumor biomarkers at the early stage enables early diagnosis of cancer and receives great deal of attentions [3,4]. Among various cancer biomarker detections, quantified detection of serum biomarkers plays a critical role not only in detecting disease at the early stage, but also in tracking disease development after medical treatment [5]. Recently, varieties of immunoassays and immunosensors are devoted to the detection of a single cancer biomarker [6][7][8]. Although they achieved low detection limits, a majority of cancers have more than one biomarker that correlated with their incidence, and some cancer biomarkers are nonspecific to a special cancer, such as α-fetoprotein (AFP) and carcinoembryonic antigen (CEA), CEA and AFP are related to many kinds of cancer, including liver cancer, colorectal cancer, lung cancer, ovarian carcinoma, breast cancer and pancreatic cancer. Moreover, due to biological diversity of man, only one biomarker detection may generate false positive, which is unreliable [9,10]. Therefore, the detection of a single cancer biomarker is inadequate in diagnosing a special cancer. Comparing with the single biomarker test, multiplex immunoassays of cancer biomarkers can promote the screening and diagnosis of some cancer-related illness [11][12][13]. In addition, the simultaneous multiplex assay that detected more than one cancer biomarker in a single experiment possesses remarkable superiorities, such as shorter analytical time, enhanced detecting throughput, higher efficiency, lower detection cost and less sample. Therefore, it would be advantageous to explore an efficient and ultras...
Surface‐enhanced Raman spectroscopy (SERS) is a very powerful vibrational method that can be applied to a variety of biological samples provided that drawbacks, such as poor reproducibility of the signal and biocompatibility of the substrates, can be satisfactorily overcome. SERS is characterized by very low limits of detection, mainly due to the electromagnetic enhancement of the Raman signals, capable of achieving the single‐molecule level. Moreover, the spectra of small molecules represent authentic molecular fingerprints with very narrow linewidths that, along with the additional enhancement and inter‐/intramolecular selectivity gained through resonant laser excitation (surface‐enhanced resonance Raman spectroscopy, SERRS), make this technique particularly suited for multiplexing applications in complex biological samples. In this article, we introduce first the physical principles of SER(R)RS and describe the different types of substrates, as well as the basic modern instrumentation. We then provide an overview of recent biological applications, including biophysical mechanistic and structural studies on isolated biomolecules, their direct and indirect detection, chemical imaging of biological samples, and biomedical applications. Finally, we briefly discuss our outlook for the future research in this field.
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