Active targeting of orthotopic glioma using biomimetic liposomes co-loaded elemene and cabazitaxel modified by transferritin

Abstract

Background Effective treatment of glioma requires a nanocarrier that can cross the blood–brain barrier (BBB) to target the tumor lesion. In the current study, elemene (ELE) and cabazitaxel (CTX) liposomes were prepared by conjugating liposomes with transferrin (Tf) and embedding the cell membrane proteins of RG2 glioma cells into liposomes (active-targeting biomimetic liposomes, Tf-ELE/CTX@BLIP), which exhibited effective BBB infiltration to target glioma. Results The findings showed that Tf-ELE/CTX@BLIP was highly stable. The liposomes exhibited highly significant homologous targeting and immune evasion in vitro and a 5.83-fold intake rate compared with classical liposome (ELE/CTX@LIP). Bioluminescence imaging showed increased drug accumulation in the brain and increased tumor penetration of Tf-ELE/CTX@BLIP in orthotopic glioma model nude mice. Findings from in vivo studies indicated that the antitumor effect of the Tf-ELE/CTX@BLIP led to increased survival time and decreased tumor volume in mice. The average tumor fluorescence intensity after intravenous administration of Tf-ELE/CTX@BLIP was 65.2, 12.5, 22.1, 6.6, 2.6, 1.5 times less compared with that of the control, CTX solution, ELE solution, ELE/CTX@LIP, ELE/CTX@BLIP, Tf-ELE/CTX@LIP groups, respectively. Histopathological analysis showed that Tf-ELE/CTX@BLIP were less toxic compared with administration of the CTX solution. Conclusion These findings indicate that the active-targeting biomimetic liposome, Tf-ELE/CTX@BLIP, is a promising nanoplatform for delivery of drugs to gliomas. Graphic Abstract

Intranasal Delivery of Liposomes to Glioblastoma by Photostimulation of the Lymphatic System

“…The liposomes are promising transporters to deliver antitumoral drugs to GBM [ 27 , 43 , 44 , 45 , 46 , 47 ]. However, there is evidence that lymphatic drainage of the brain is impaired in animals with GBM [ 57 , 58 ].…”

“…Liposomes are promising transporters to deliver anti-cancer drugs to GBM [ 27 , 43 , 44 , 45 , 46 , 47 ]. They offer numerous advantages, such as the similarity of the liposomal bilayer with the cell membrane; liposomes provide low drug toxicity, stability, and increased circulation time as well as targeting liposomes by modifying with antibodies, and polypeptides [ 43 , 48 , 49 ].…”

Low-Level Laser Treatment Induces the Blood-Brain Barrier Opening and the Brain Drainage System Activation: Delivery of Liposomes into Mouse Glioblastoma

“…Therefore, the development of effective methods bypassing the IBBB at the border of GBM is the first step in the GBM therapy. Liposomes are promising nanocarriers to deliver anti-cancer drugs to GBM [ 37 , 68 , 69 , 70 , 71 ]. Therefore, in the final step, we studied the 1268 nm laser-induced BBBO for the delivery of liposomes into GBM.…”

Intelligent Nanoparticles With pH-Sensitive Co-Delivery of Temozolomide and siEGFR to Ameliorate Glioma Therapy

“…In glioma therapy, TMZ and siEGFR encapsulated within NPs must be released into the glioma cells in order to exhibit a synergistic anticancer effect. Researchers have modified a targeting ligand-transferrin (Tf) liposome to specifically recognize transferrin receptors (TfR), increasing brain drug delivery ( Li et al, 2021 ). In this study, Tf was modified on the surface of PEG-PAE(SS)/TMZ@siEGFR to increase the BBB penetrating efficiency and the glioma distribution of drugs.…”