Active oral immunization of suckling piglets to prevent colonization after weaning by enterotoxigenic Escherichia coli with fimbriae F18

Bertschinger, H. U.; Nief, Vera; Tschäpe, Helmut

doi:10.1016/s0378-1135(99)00166-2

Cited by 43 publications

(33 citation statements)

References 13 publications

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“…Such organization of the GALT may provide immune protection at mucosal surfaces where the infection actually occurs (McGhee et al, 1992). Bertschinger et al (2000) demonstrated the protective effects of a live oral vaccine containing F18 fimbria against porcine postweaning diarrhea and oedema disease.…”

Section: P Orcine Colidiarrhea and Colienterotoxemia Induced With F4mentioning

confidence: 99%

Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with vaccine candidate F18ac+ nonenterotoxigenic E. coli strain

Janjatović¹,

Lackovic²,

Bozic³

et al. 2009

Eur J Histochem

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Colidiarrhea and colienterotoxemia caused by F4+ and/or F18 + enterotoxigenic E. coli (ETEC) strains are the most prevalent infections of suckling and weaned pigs. Here we tested the immunogenicity and protective effectiveness of attenuated F18ac + non-ETEC vaccine candidate strain against challenge infection with F4ac + ETEC strain by quantitative phenotypic analysis of small intestinal leukocyte subsets in weaned pigs.We also evaluated levamisole as an immune response modifier (IRM) and its adjuvanticity when given in the combination with the experimental vaccine. The pigs were parenterally immunized with either levamisole (at days -2, -1 and 0) or with levamisole and perorally given F18ac + and myeloid SWC3 + cell subsets were identified in jejunal and ileal epithelium, lamina propria and Peyer's patches using the avidin-biotin complex method, and their numbers were determined by computer-assisted histomorphometry. Quantitative immunophenotypic analyses showed that levamisole treated pigs had highly increased numbers of jejunal CD3 + , CD45RC + and SWC3 + cells (p<0.05) as compared to those recorded in nontreated control pigs.In the ileum of these pigs we have recorded that only CD21 + cells were significantly increased (p<0.01). The pigs that were treated with levamisole adjuvanted experimental vaccine had significantly increased numbers of all tested cell subsets in both segments of the small intestine. It was concluded that levamisole adjuvanted F18ac + non-ETEC vaccine was a requirement for the elicitation of protective gut immunity in this model; nonspecific immunization with levamisole was less effective, but confirmed its potential as an IRM.

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Section: P Orcine Colidiarrhea and Colienterotoxemia Induced With F4mentioning

confidence: 99%

Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with vaccine candidate F18ac+ nonenterotoxigenic E. coli strain

Janjatović¹,

Lackovic²,

Bozic³

et al. 2009

Eur J Histochem

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“…The gene encoding F18 adhesin is one of the most important to have been recently associated with post-weaning diarrhoea and with oedema disease (Bertschinger et al, 2000;Frydendahl, 2002;Vu-Khac et al, 2006 The F17 adhesin gene of E. coli has been isolated frequently from calves with or without diarrhoea. We found it in approximately 9 % of the isolates from piglets with diarrhoea, most of them from suckling piglets.…”

Section: Haemolytic Activity In Strains Of E Colimentioning

confidence: 99%

“…F4 and F18 are the fimbrial adhesins of ETEC associated with post-weaning diarrhoea (Ojeniyi et al, 1994;Imberechts et al, 1997;Bertschinger et al, 2000;Vu-Khac et al, 2007;Kim et al, 2010). F18 is the fimbrial adhesin most commonly found on Shiga toxin-producing E. coli (STEC) causing oedema disease (Vu-Khac et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Toledo

Gómez

Cruz

et al. 2012

Journal of Medical Microbiology

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Faecal Escherichia coli isolates from suckling (n5503) and weaning (n5450) piglets with and without diarrhoea from 10 farms in Mexico were examined for identification and prevalence of virulence genes. E. coli isolates were tested further for enterotoxin (LT, STa, STb, Stx1, Stx2 and EAST1), fimbrial (F4, F5, F6, F17, F18 and F41) and eae adhesin genes by multiplex PCR. Of the 953 isolates of E. coli examined by multiplex PCR, 650 (68.2 %) isolates were positive for at least one adhesin gene. Among the isolates from diarrhoeic piglets, F41 (72 %) was the most prevalent adhesin followed by eae adhesin (27 %), F6 (12 %), F17 (9 %), F18 (9 %), F5 (8 %) and F4 (3 %). Enterotoxin genes were detected in 424 (44.5 %) of the isolates, of which EAST1 (38 %) and STa (30 %) were the most common, followed by STb (17 %), Stx2 (6 %) and LT (5 %). Twenty-three per cent of isolates from suckling piglets and 43 % of isolates from weaned piglets carried both enterotoxin and adhesin genes, the most common virotypes being F41 : STa, F41 : EAST1, EAST1 : eae, F41 : F6, F41 : STa : STb, F41 : eae and F17 : eae. The present study examined for the first time, to our knowledge, the prevalence of 13 virulence genes among E. coli strains isolated from piglets with and without diarrhoea in Mexico. The results suggested that there are a wide variety of virulence genes associated with diarrhoea in piglets. This study provides baseline information on the significance of specific virotypes associated with suckling and weaning periods in piglets in Mexico.

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“…Subunit A has N-glycosidase activity, which can specifically break down the glycosidic bond of the adenine located at position 4324 of the 5ʹ-end of 28S rRNA. The unlinked adenine residues then prevent binding between amino-acylation tRNA on elongation factor EF-1 and the 60S mitochondrial subunit, and subsequently interrupt protein synthesis, which induces metabolic dysfunction and cell death (DeGrandis et al, 1987;Imberechts et al, 1992;Bertschinger et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice

Yang¹,

Liu²,

Hu³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The exotoxin SLT-IIeB from the Escherichia coli Ee strain was expressed in E. coli, and the recombinant protein was purified, mixed with the Ee strain, then emulsified with oil-emulsion adjuvants to obtain a mixed subunit bacterin. Groups of Kunming mice were immunized at weeks 0 and 2, and challenged intraperitoneally with the Ee strain at week 4. Antibodies were detected by ELISA and an agglutination test. After the second immunization, the antibody level increased and the rate of immune protection against the Ee strain was 70 and 91.7% in the subunit bacterin and bacterin groups, respectively. Therefore, the mixed subunit bacterin provided good protection against the homologous Ee strain, which provides a basis for further research, 18027Genetic subunit bacterin against edema disease ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18026-18033 (2015) into high-efficacy vaccines against porcine edema disease.

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Active oral immunization of suckling piglets to prevent colonization after weaning by enterotoxigenic Escherichia coli with fimbriae F18

Cited by 43 publications

References 13 publications

Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with vaccine candidate F18ac+ nonenterotoxigenic E. coli strain

Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with vaccine candidate F18ac+ nonenterotoxigenic E. coli strain

Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico

Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice

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