The proposed paper, presents the construction of adaptive noise cancellation filter based on gray wolf optimization (GWO) optimization technique.The relative investigation of different strategies uncovers that the presentation of GWO calculation is better in boisterous condition. The objective of proposed paper is structure ANC channel utilizing GWO method that improves association involving output with pure EMG signal.The results of proposed strategy are contrasted through gray wolf optimizer (GWO) and other evolutionary algorithms.The presentation of these calculations is assessed regarding signal-to-noise ratio (S SNR), mean square error (S MSE), maximum error (S ME) mean, convergence rate (CR) plus correlation feature (S r). The noise attenuation capability is tested on EMG signal contaminated with power line and ECG noise at different SNR levels. The ANC filter based on GWO technique provides 28 dB improvement in output SNR, 81% reduction in MSE, and 84% lower ME as compared to reported ANC filter based on RLS algorithm. Further, ANC filter based on GWO technique provides 7 dB improvement in output SNR, 59.5% reduction in MSE, and 69.2% lower ME as compared to recently reported ANC filter based on ABC-MR algorithm.