Active microfluidic systems for cell sorting and separation

Sivaramakrishnan, Muthusaravanan; Kothandan, Ram; Govindarajan, Deenadayalan Karaiyagowder; Meganathan, Yogesan; Kandaswamy, Kumaravel

doi:10.1016/j.cobme.2019.09.014

Cited by 61 publications

(28 citation statements)

References 68 publications

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“…96 The acoustophoresis devices are excellent representations of the use of microfluidic channels that allow an accurate and efficient method of a volume-dependent ultrasonic cell separation technique with a multistream laminar-flow designed for high purity separation. 56,97 This method's cell cycle phase synchrony level is comparable to other cell cycle synchronisation techniques like centrifugal elutriation. 98 Higher purities and throughput have been achieved by optimising the channel geometry, serial integration of the device, and parallel operation.…”

Section: Acoustophoresismentioning

confidence: 84%

Advances and enabling technologies for phase-specific cell cycle synchronisation

et al. 2022

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Section: Acoustophoresismentioning

confidence: 84%

Advances and enabling technologies for phase-specific cell cycle synchronisation

et al. 2022

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“…Therefore, there has been an urgent need for an effective sorting method to recover the desired droplets for downstream processing. Since it has a technological importance for many other fields, single cell sorting has witnessed an array of enabling tools involving different mechanisms, such as electrokinetics, acoustophoresis, optics, mechanics, hydrodynamics, and magnetophoretics, which are summarized and intensively discussed this review [ 87 ].…”

Section: Analysis and Monitoring Of Microorganisms In Dropletsmentioning

confidence: 99%

Droplet microfluidics on analysis of pathogenic microbes for wastewater-based epidemiology

Cao

Zhang

et al. 2021

TrAC Trends in Analytical Chemistry

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Infectious diseases caused by pathogenic microbes have posed a major health issue for the public, such as the ongoing COVID-19 global pandemic. In recent years, wastewater-based epidemiology (WBE) is emerging as an effective and unbiased method for monitoring public health. Despite its increasing importance, the advancement of WBE requires more competent and streamlined analytical platforms. Herein we discuss the interactions between WBE and droplet microfluidics, focusing on the analysis of pathogens in droplets, which is hard to be tackled by traditional analytical tools. We highlight research works from three aspects, namely, quantitation of pathogen biomarkers in droplets, single-cell analysis in droplets, and living cell biosensors in droplets, as well as providing future perspectives on the synergy between WBE and droplet microfluidics.

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“…They offer the potential advantages of reduced sample consumption [2,3], high sensitivity, and ease of mass production compared with traditional separation techniques, such as centrifugation and filtration. Microfluidic technology is now widely used in medical diagnosis [4], biological detection [5], chemical analysis [6], and other aspects where particle and cell separations are critical to numerous applications. Many techniques have been developed in microfluidics, including inertial microfluidics [7], deterministic lateral displacement [8], hydrophoresis [9,10], optical [11], dielectrophoresis (DEP) [12], surface acoustic waves [13], and magnetic force to achieve precise control and sorting of detection objects such as particles and cells with microfluidic chips.…”

Section: Introductionmentioning

confidence: 99%

Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force

Duan

et al. 2022

Micromachines

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Cell separation has become @important in biological and medical applications. Dielectrophoresis (DEP) is widely used due to the advantages it offers, such as the lack of a requirement for biological markers and the fact that it involves no damage to cells or particles. This study aimed to report a novel approach combining 3D sidewall electrodes and contraction/expansion (CEA) structures to separate three kinds of particles with different sizes or dielectric properties continuously. The separation was achieved through the interaction between electrophoretic forces and inertia forces. The CEA channel was capable of sorting particles with different sizes due to inertial forces, and also enhanced the nonuniformity of the electric field. The 3D electrodes generated a non-uniform electric field at the same height as the channels, which increased the action range of the DEP force. Finite element simulations using the commercial software, COMSOL Multiphysics 5.4, were performed to determine the flow field distributions, electric field distributions, and particle trajectories. The separation experiments were assessed by separating 4 µm polystyrene (PS) particles from 20 µm PS particles at different flow rates by experiencing positive and negative DEP. Subsequently, the sorting performances of the 4 µm PS particles, 20 µm PS particles, and 4 µm silica particles with different solution conductivities were observed. Both the numerical simulations and the practical particle separation displayed high separating efficiency (separation of 4 µm PS particles, 94.2%; separation of 20 µm PS particles, 92.1%; separation of 4 µm Silica particles, 95.3%). The proposed approach is expected to open a new approach to cell sorting and separating.

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Active microfluidic systems for cell sorting and separation

Cited by 61 publications

References 68 publications

Advances and enabling technologies for phase-specific cell cycle synchronisation

Advances and enabling technologies for phase-specific cell cycle synchronisation

Droplet microfluidics on analysis of pathogenic microbes for wastewater-based epidemiology

Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force

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