This article describes the methodological approach of systemic psychophysiology. In the framework of this approach a wide range of experimental data is analyzed: results of neuronal recordings in vitro and in awake normal and pathological animals learning to perform and performing both complex instrumental and simple behavioral acts. Another block of analyzed data is based on experiments with human subjects who learn and perform the tasks of categorization of words and operator tasks, participate in group game activity, and answer the questionnaires of psychodiagnostic methods. As a result of this analysis, the systemic psychophysiology approach is used to describe qualitatively and quantitatively the formation and realization of individual experience.