Active-Matrix Digital Microfluidics Design and Optimization for High-Throughput Droplets Manipulation

Wang, Dongping; Wu, Shiqiang; Huang, Qiaorong; Chang, Chunyu; Xu, Longqian; Jin, Kai; Hu, Siyi; Yu, Jun; Ma, Hanbin

doi:10.1109/jeds.2023.3293234

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…However, with the development of the digital microfluidic technology, the number of electrodes has been further increased to achieve higher parallel processing capabilities. In our previous work, the number of electrodes in a chip was 4096, with a single droplet volume of 10 nL. , Existing temperature control methods cannot meet the requirements of high-throughput DMF chips, especially for scenarios such as droplet digital PCR and single-cell genomics that require temperature control for a large number of droplets. , These scenarios require the consideration of the planar and vertical temperature distribution inside the chip, which has not been previously studied. Therefore, in DMF technology, an open thermal control system that ensures precise temperature control over a wide range is desirable.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous work, the number of electrodes in a chip was 4096, with a single droplet volume of 10 nL. 22 , 23 Existing temperature control methods cannot meet the requirements of high-throughput DMF chips, especially for scenarios such as droplet digital PCR and single-cell genomics that require temperature control for a large number of droplets. 24 , 25 These scenarios require the consideration of the planar and vertical temperature distribution inside the chip, which has not been previously studied.…”

Section: Introductionmentioning

confidence: 99%

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Open Thermal Control System for Stable Polymerase Chain Reaction on a Digital Microfluidic Chip

Ji,

Hu,

Pang

et al. 2024

ACS Omega

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In this paper, a digital microfluidic thermal control system was introduced for the stable polymerase chain reaction (PCR). The system consists of a thermoelectric cooler unit, a thermal control board, and graphical-user-interface software capable of simultaneously achieving temperature control and on-chip droplet observation. A fuzzy proportional-integralderivative (PID) method was developed for this system. The simulation analysis was performed to evaluate the temperature of different reagents within the chip. Based on the results, applying fuzzy PID control for PCR will enhance the thermal stability by 67.8% and save the time by 1195 s, demonstrating excellent dynamic response capability and thermal robustness. The experimental results are consistent with the simulation results on the planar temperature distribution, with a data consistency rate of over 99%. The PCR validation was carried out on this system, successfully amplifying the rat GAPDH gene at a concentration of 193 copies/μL. This work has the potential to be useful in numerous existing lab-on-a-chip applications.

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