Active immunization of hamsters against experimental infection with Borrelia burgdorferi

Johnson, Russell C.; Kodner, Carrie; Russell, Marie

doi:10.1128/iai.54.3.897-898.1986

Cited by 82 publications

(49 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings and those of Johnson et al (10)(11)(12)(13) are important. They demonstrate unequivocally that antibodymediated immunity plays an important role against infection with B. burgdorferi.…”

Section: Discussionsupporting

confidence: 53%

See 1 more Smart Citation

Passive immunization prevents induction of Lyme arthritis in LSH hamsters

et al. 1990

View full text Add to dashboard Cite

We determined that sera obtained from hamsters infected with Borrelia burgdorferi could prevent the induction of Lyme arthritis. When irradiated hamsters were administered immune serum and subsequently challenged with B. burgdorferi, no evidence of infection was detected. Recipients failed to develop swelling of the hind paws, and no histopathologic changes were detected. In addition, B. burgdorferi was not recovered from tissues of hamsters that were passively immunized. By contrast, irradiated hamsters that were administered normal hamster serum or saline and infected with the Lyme spirochete developed arthritis. Extensive histopathologic changes occurred in the hind paws and knee joints, and spirochetes were recovered from most of the tissues examined. These results show that immune serum can confer complete protection on recipient hamsters to challenge with B. burgdorferi.

show abstract

Section: Discussionsupporting

confidence: 53%

“…B. burgdorferi 297 was obtained from Russell C. Johnson (University of Minnesota, Minneapolis). The strain was originally isolated from human spinal fluid (24) and has been maintained by passage in Barbour-Stoenner-Kelly medium (BSK) and hamsters (10)(11)(12)(13)(14)19).…”

mentioning

confidence: 99%

Passive immunization prevents induction of Lyme arthritis in LSH hamsters

et al. 1990

View full text Add to dashboard Cite

show abstract

“…B. burgdorferi 297 was obtained from Russell C. Johnson (University of Minnesota, Minneapolis). The strain was originally isolated from human spinal fluid (26) and has been maintained by passage in modified Barbour-Stoenner-Kelly medium (BSK; 5) and hamsters (11)(12)(13)(14)(15)21). The hamster-passed spirochetes were grown in BSK at 35°C for 5 days.…”

Section: Methodsmentioning

confidence: 99%

Borreliacidal activity of sera from hamsters infected with the Lyme disease spirochete

et al. 1991

View full text Add to dashboard Cite

An in vitro borreliacidal assay that accurately reflects the levels of protective antibody determined by passive transfer of immunity studies was developed. Borreliacidal antibody in sera obtained from normal hamsters infected with Borrelia burgdorferi was readily detected. When immune serum containing complement was incubated with B. burgdorferi organisms, spirochetes were killed within 2 h. Treating immune serum with anti-hamster immunoglobulin G abrogated the borreliacidal activity. Killing of B. burgdorferi in serum was detected 1 week after infection; it peaked at week 3 and gradually declined. Relatively high levels of borreliacidal antibody were found, especially in week 3 immune serum, which could be diluted 1,280-fold. The decrease in borreliacidal antibody after infection may account for occurrences of reinfection and the remitting course of Lyme disease.

show abstract

“…Acquired immunity in the vertebrate host plays an important role in the epidemiology of Lyme disease (Johnson et al ., 1986a,b; Kurtenbach et al ., ; Piesman et al ., ; Liang et al ., ). One Borrelia antigen that is particularly important for the pathogen's interaction with the vertebrate immune system is outer surface protein C (OspC) (Radolf and Caimano, ).…”

Section: Introductionmentioning

confidence: 99%

Strain‐specific antibodies reduce co‐feeding transmission of the Lyme disease pathogen, Borrelia afzelii

Jacquet

Durand

Rais

et al. 2015

Environmental Microbiology

View full text Add to dashboard Cite

Vector-borne pathogens use a diversity of strategies to evade the vertebrate immune system. Co-feeding transmission is a potential immune evasion strategy because the vector-borne pathogen minimizes the time spent in the vertebrate host. We tested whether the Lyme disease pathogen, Borrelia afzelii, can use co-feeding transmission to escape the acquired immune response in the vertebrate host. We induced a strain-specific, protective antibody response by immunizing mice with one of two variants of OspC (A3 and A10), the highly variable outer surface protein C of Borrelia pathogens. Immunized mice were challenged via tick bite with B. afzelii strains A3 or A10 and infested with larval ticks at days 2 and 34 post-infection to measure co-feeding and systemic transmission respectively. Antibodies against a particular OspC variant significantly reduced co-feeding transmission of the targeted (homologous) strain but not the non-targeted (heterologous) strain. Cross-immunity between OspC antigens had no effect in co-feeding ticks but reduced the spirochaete load twofold in ticks infected via systemic transmission. In summary, OspC-specific antibodies reduced co-feeding transmission of a homologous but not a heterologous strain of B. afzelii. Co-feeding transmission allowed B. afzelii to evade the negative consequences of cross-immunity on the tick spirochaete load.

show abstract

Active immunization of hamsters against experimental infection with Borrelia burgdorferi

Cited by 82 publications

References 14 publications

Passive immunization prevents induction of Lyme arthritis in LSH hamsters

Passive immunization prevents induction of Lyme arthritis in LSH hamsters

Borreliacidal activity of sera from hamsters infected with the Lyme disease spirochete

Strain‐specific antibodies reduce co‐feeding transmission of the Lyme disease pathogen, Borrelia afzelii

Contact Info

Product

Resources

About