Active immunisation of mice with GnRH lipopeptide vaccine candidates: Importance of T helper or multi-dimer GnRH epitope

Goodwin, Daryn; Simerská, Pavla; Chang, Chiung-Hsin; Mansfeld, Friederike M.; Varamini, Pegah; D’Occhio, Michael J.; Tóth, István

doi:10.1016/j.bmc.2014.06.052

Cited by 16 publications

(7 citation statements)

References 42 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…adjuvants are associated with a higher immune response, but CFA demonstrated a better immune response than Al(OH)3 (Goodwin et al, 2014). In the case of vaccines supplemented with CFA and Titermax, both adjuvants induced similar T levels but the former produced a relatively strong inflammatory response (Cribbs et al, 2003).…”

Section: Research Articlementioning

confidence: 99%

Effect of Active Immunization Using Gonadotropin-Releasing Hormone 2-Multiple Antigen Peptide with Different Adjuvants on the Reproductive Functions of Male Rats

Si¹

2019

PVJ

View full text Add to dashboard Cite

This study was aimed to compare the effects of different adjuvants on male reproductive function in rats immunized using GnRH2-MAP. Male Sprague-Dawley rats were assigned to control, ISA 201 adjuvant and Gel 01 adjuvant groups along with GnRH2-MAP. The serum testosterone and antibody titers were measured every 2-week by ELISA, the testes were weighed and histologically analyzed. mRNA expression levels in the hypothalamus, pituitary, and testes were determined by RT-qPCR. Among the three groups, the ISA 201 adjuvant group exhibited the lowest testosterone concentration (P<0.05), the highest antibody titer (P<0.05), and testis atrophy. GnRH mRNA expression in the hypothalamus; GnRH-R, FSH-β, and LH-β expression in the pituitary; and FSH-R, LH-R, and 3β-HSD expression in the testes were significantly lower in this group (P<0.01). Additionally, several other genes showed significant differential expression in the ISA 201 adjuvant group than in the other two groups. It is concluded that the GnRH2-MAP vaccine emulsified with ISA 201 adjuvant is more effective than that administered with Gel 01 adjuvant. , 2019. Effect of active immunization using gonadotropin-releasing hormone 2-multiple antigen peptide with different adjuvants on the reproductive functions of male rats. Pak Vet J, 39(2): 205-210. http://dx.

show abstract

Section: Research Articlementioning

confidence: 99%

Effect of Active Immunization Using Gonadotropin-Releasing Hormone 2-Multiple Antigen Peptide with Different Adjuvants on the Reproductive Functions of Male Rats

Si¹

2019

PVJ

View full text Add to dashboard Cite

show abstract

“…After dividing the resin, the lysine Fmoc protecting group was removed using piperidine, and the lipidic adjuvanting moiety could be incorporated (Scheme 2). The lipidic adjuvanting moieties of glyco-lipopeptides 3a and 3b have been prepared previously using Boc [6,8] or Dde [31] protected synthetic LAAs. In this case, Dde protected LAAs were employed (Dde-C12-OH, Dde-C16-OH) and prepared according to the procedure of Ross et al [32] The lipidated lysine amino acid (Fmoc- 10.1002/cbic.201600639 ChemBioChem K(C12)-OH) was synthesized according to the procedure reported by Fagan et al, [13] and was used to prepare the lipidic sequence of glyco-lipopeptide 3c by standard Fmoc SPPS.…”

Section: Synthesismentioning

confidence: 99%

Synthesis, Characterization and Immunological Evaluation of Self‐Adjuvanting Group A Streptococcal Vaccine Candidates Bearing Various Lipidic Adjuvanting Moieties

Fagan

Hussein

et al. 2017

ChemBioChem

Self Cite

View full text Add to dashboard Cite

Four group A streptococcal glyco-lipopeptide vaccine candidates were prepared and characterized, each possessing different lipidic adjuvanting moieties. The immunogenicity of the compounds was evaluated by macrophage and dendritic cell uptake studies and by in vivo quantification of systemic IgG antibody using ELISA. Three of the four vaccine candidates showed a significant induction of IgG response compared to a negative control.

show abstract

“…[ d ‐Lys 6 ]‐GnRH and [Gln 1 , d ‐Lys 6 , des‐Gly 10 , Pro 9 ‐NHEt]‐GnRH (Gln‐His‐Trp‐Ser‐Tyr‐ d ‐Lys‐Leu‐Arg‐Pro‐NHEt), both of which have a reactive ε‐amino group, have been used as delivery vehicles to target various diagnostic and chemotherapeutic agents to tumor cells that overexpress GnRH receptors . The D ‐cysteine modified GnRH analogs, including [ d ‐Cys 6 ]‐GnRH and a shortened heptapeptide (pGlu‐His‐Trp‐Ser‐Tyr‐ d ‐Cys‐Leu‐OH), were also used as building blocks for GnRH‐based immunotherapeutic agents or targeting ligands for selected drug delivery . Based on these findings, it is hypothesized that incorporation of d ‐Cys 6 substitution and C‐terminus amidated modification may afford GnRH analogs that can be potentially used as GnRH receptor targeting peptides.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, in vitro stability, and antiproliferative effect of d‐cysteine modified GnRH‐doxorubicin conjugates

Zhao

Chang

et al. 2018

Journal of Peptide Science

View full text Add to dashboard Cite

Overexpression of gonadotropin-releasing hormone (GnRH) receptor in many tumors but not in normal tissues makes it possible to use GnRH analogs as targeting peptides for selective delivery of cytotoxic agents, which may help to enhance the uptake of anticancer drugs by cancer cells and reduce toxicity to normal cells. The GnRH analogs [D-Cys 6 , desGly 10 , Pro 9 -NH 2 ]-GnRH, [D-Cys 6 , desGly 10 , Pro 9 -NHEt]-GnRH, and [D-Cys 6 , α-aza-Gly 10 -NH 2 ]-GnRH were conjugated with doxorubicin (Dox), respectively, through N-succinimidyl-3-maleimidopropionate as a linker to afford three new GnRH-Dox conjugates. The metabolic stability of these conjugates in human serum was determined by RP-HPLC. The antiproliferative activity of the conjugates was examined in GnRH receptor-positive MCF-7 human breast cancer cell line by MTT assay. The three GnRH-Dox conjugates showed improved metabolic stability in human serum in comparison with AN-152. The antiproliferative effect of conjugate II ([D-Cys 6 , desGly 10 , Pro 9 -NHEt]-GnRH-Dox) on MCF-7 cells was higher than that of conjugate I ([D-Cys 6 , desGly 10 , Pro 9 -NH 2 ]-GnRH-Dox) and conjugate III ([D-Cys 6 , α-aza-Gly 10 -NH 2 ]-GnRH-Dox), and the cytotoxicity of conjugate II against GnRH receptor-negative 3T3 mouse embryo fibroblast cells was decreased in comparison with free Dox. GnRH receptor inhibition test suggested that the antiproliferative activity of conjugate II might be due to the cellular uptake mediated by the targeting binding of [D-Cys 6 -des-Gly 10 -Pro 9 -NHEt]-GnRH to GnRH receptors. Our study indicates that targeting delivery of conjugate II mediated by [D-Cys 6 -des-Gly 10 -Pro 9 -NHEt]-GnRH is a promising strategy for chemotherapy of tumors that overexpress GnRH receptors.

show abstract

Active immunisation of mice with GnRH lipopeptide vaccine candidates: Importance of T helper or multi-dimer GnRH epitope

Cited by 16 publications

References 42 publications

Effect of Active Immunization Using Gonadotropin-Releasing Hormone 2-Multiple Antigen Peptide with Different Adjuvants on the Reproductive Functions of Male Rats

Effect of Active Immunization Using Gonadotropin-Releasing Hormone 2-Multiple Antigen Peptide with Different Adjuvants on the Reproductive Functions of Male Rats

Synthesis, Characterization and Immunological Evaluation of Self‐Adjuvanting Group A Streptococcal Vaccine Candidates Bearing Various Lipidic Adjuvanting Moieties

Synthesis, in vitro stability, and antiproliferative effect of d‐cysteine modified GnRH‐doxorubicin conjugates

Contact Info

Product

Resources

About