Active growth factor delivery from poly(d,l-lactide-co-glycolide) foams prepared in supercritical CO2

Hile, David D.; Amirpour, Mary Lee; Akgerman, Aydin; Pishko, Michael V.

doi:10.1016/s0168-3659(99)00268-0

Cited by 191 publications

(111 citation statements)

References 27 publications

Supporting

Mentioning

105

Contrasting

Unclassified

Order By: Relevance

“…Natural materials include gelatin [31,32], collagen [33], fibrin [34], heparin [35] and alginate. [36] Synthetic matrices have been generated from poly(ethylene glycol)-g-poly(lactic acid) [37], poly(lactic acid) [38], poly(lactic-co-glycolic acid) [39,40], and methylidene malonate polymers. [41] From a mechanical perspective, these scaffolds have ranged from relatively weak materials (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…A bFGF loaded PLGA scaffold fabricated using supercritical CO 2 exhibited 45% initial burst release in one day. [39,40] The release of bFGF from methylidene malonate polymer films had over 30% initial burst release in the first day. [41] In both of these studies the bioactivity of the released bFGF was verified with cellular mitogenicity assays similar to those employed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier work with bFGF loaded PLGA scaffolds suggested that an inappropriately harsh solvent could lead to substantial loss of growth factor bioactivity. [40] In this work, DMSO was used due to its relative high melting point (18°C) and lower toxicity relative to other solvents such as methylene chloride, a commonly used solvent for preparation of growth factor loaded microspheres. Moreover, DMSO is commonly used in cell preservation procedures.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Guan

Stankus

Wagner

2007

Journal of Controlled Release

149

View full text Add to dashboard Cite

Scaffolds that better approximate the mechanical properties of cardiovascular and other soft tissues might provide a more appropriate mechanical environment for tissue development or healing in vivo. An ability to induce local angiogenesis by controlled release of an angiogenic factor, such as basic fibroblast growth factor (bFGF), from a biodegradable scaffold with mechanical properties more closely approximating soft tissue could find application in a variety of settings. Toward this end biodegradable poly(ester urethane)urea (PEUU) scaffolds loaded with bFGF were fabricated by thermally induced phase separation. Scaffold morphology, mechanical properties, release kinetics, hydrolytic degradation and bioactivity of the released bFGF were assessed. The scaffolds had interconnected pores with porosities of 90% or greater and pore sizes ranging from 34-173 μm. Scaffolds had tensile strengths of 0.25-2.8 MPa and elongations at break of 81-443%. Incorporation of heparin into the scaffold increased the initial burst release of bFGF, while the initial bFGF loading content did not change release kinetics significantly. The released bFGF remained bioactive over 21 days as assessed by smooth muscle mitogenicity. Scaffolds loaded with bFGF showed slightly higher degradation rates than unloaded control scaffolds. Smooth muscle cells seeded into the scaffolds with bFGF showed higher cell densities than for control scaffolds after 7 days of culture. The bFGFreleasing PEUU scaffolds thus exhibited a combination of mechanical properties and bioactivity that might be attractive for use in cardiovascular and other soft tissue applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Guan

Stankus

Wagner

2007

Journal of Controlled Release

149

View full text Add to dashboard Cite

show abstract

“…These methods can achieve certain slow release characteristics, but the control over release kinetics is limited. The gas foaming process can entrap PLGA microspheres in a porous scaffold [138,139]. In addition to the lack of control over pore size and shape, the release kinetics of imbedded microspheres in the matrix walls are uncontrollably changed from that by the microspheres.…”

Section: Bioactive Molecule Deliverymentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,201

819

View full text Add to dashboard Cite

Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

show abstract

“…Moreover, open frames are designed with defined parameters to improve the osteoconductivity, such as porosity, pore size, and connectivity, as well as enhanced mechanical properties [3]. Furthermore, this technique allows producing biocompatible samples without the use of potentially toxic organic solvents [4,5]. These structures were recently tested in vivo for host tissue induced reactions as well as for their osteoconductive properties [6].…”

Section: Introductionmentioning

confidence: 99%

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Montjovent

Mark

Mathieu

et al. 2008

Bone

View full text Add to dashboard Cite

Fetal bone cells were shown to have an interesting potential for therapeutic use in bone tissue engineering due to their rapid growth rate and their ability to differentiate into mature osteoblasts in vitro. We describe hereafter their capability to promote bone repair in vivo when combined with porous scaffolds based on poly(L-lactic acid) (PLA) obtained by supercritical gas foaming and reinforced with 5 wt.% β-tricalcium phosphate (TCP).Bone regeneration was assessed by radiography and histology after implantation of PLA/TCP scaffolds alone, seeded with primary fetal bone cells, or coated with demineralized bone matrix. Craniotomy critical size defects and drill defects in the femoral condyle in rats were employed. In the cranial defects, polymer degradation and cortical bone regeneration were studied up to 12 months postoperatively. Complete bone ingrowth was observed after implantation of PLA/TCP constructs seeded with human fetal bone cells. Further tests were conducted in the trabecular neighborhood of femoral condyles, where scaffolds seeded with fetal bone cells also promoted bone repair.We present here a promising approach for bone tissue engineering using human primary fetal bone cells in combination with porous PLA/TCP structures. Fetal bone cells could be selected regarding osteogenic and immune-related properties, along with their rapid growth, ease of cell banking and associated safety.

show abstract

Active growth factor delivery from poly(d,l-lactide-co-glycolide) foams prepared in supercritical CO2

Cited by 191 publications

References 27 publications

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Biomimetic materials for tissue engineering

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Contact Info

Product

Resources

About