Active Diffusion of Nanoparticles of Maternal Origin Within the Embryonic Brain

Yuryev, Mikhail; Ferreira, Mónica P. A.; Balasubramanian, Vimalkumar; Correia, Alexandra; Mäkilä, Ermei; Jokinen, Ville; Andriichuk, Liliia; Kemell, Marianna; Salonen, Jarno; Hirvonen, Jouni; Santos, Hélder A.; Rivera, Claudio

doi:10.2217/nnm-2016-0207

Cited by 12 publications

(2 citation statements)

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“…We recently developed an in vivo two-photon method to image calcium activity in the mouse embryonic cortex 24 , 25 . We previously showed that there is a remarkable selectivity of the loading of the Fluo-4AM dye for cell populations closer to the cortical plate.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, only the native in vivo environment can reflect the full complexity of interactions between blood flow and the active cells. Recent significant advances in the field of two-photon imaging 16 , 17 , 24 , 25 , 32 , 33 , allow the imaging of developmental processes while maintaining physiological conditions of live embryos. In the present work, we implement this method for calcium imaging of the cortex in embryos connected to the mother through the umbilical cord.…”

Section: Discussionmentioning

confidence: 99%

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In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity

Yuryev

Andriichuk

Leiwe

et al. 2018

Sci Rep

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Prior to sensory experience spontaneous activity appears to play a fundamental role in the correct formation of prominent functional features of different cortical regions. The use of anaesthesia during pregnancy such as ketamine is largely considered to negatively affect neuronal development by interfering with synaptic transmission. Interestingly, the characteristics of spontaneous activity as well as the acute functional effects of maternal anaesthesia remain largely untested in the embryonic cortex in vivo. In the present work, we performed in vivo imaging of spontaneous calcium activity and cell motility in the marginal zone of the cortex of E14-15 embryos connected to the mother. We made use of a preparation where the blood circulation from the mother through the umbilical cord is preserved and fluctuations in intracellular calcium in the embryonic frontal cortex are acquired using two-photon imaging. We found that spontaneous transients were either sporadic or correlated in clusters of neuronal ensembles at this age. These events were not sensitive to maternal isoflurane anaesthesia but were strongly inhibited by acute in situ or maternal application of low concentration of the anaesthetic ketamine (a non-competitive antagonist of NMDA receptors). Moreover, simultaneous imaging of cell motility revealed a correlated strong sensitivity to ketamine. These results show that anaesthetic compounds can differ significantly in their impact on spontaneous early cortical activity as well as motility of cells in the marginal zone. The effects found in this study may be relevant in the etiology of heightened vulnerability to cerebral dysfunction associated with the use of ketamine during pregnancy.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity

Yuryev

Andriichuk

Leiwe

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

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Tailoring Porous Silicon for Biomedical Applications: From Drug Delivery to Cancer Immunotherapy

et al. 2018

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In the past two decades, porous silicon (PSi) has attracted increasing attention for its potential biomedical applications. With its controllable geometry, tunable nanoporous structure, large pore volume/high specific surface area, and versatile surface chemistry, PSi shows significant advantages over conventional drug carriers. Here, an overview of recent progress in the use of PSi in drug delivery and cancer immunotherapy is presented. First, an overview of the fabrication of PSi with various geometric structures is provided, with particular focus on how the unique geometry of PSi facilitates its biomedical applications, especially for drug delivery. Second, surface chemistry and modification of PSi are discussed in relation to the strengthening of its performance in drug delivery and bioimaging. Emerging technologies for engineering PSi-based composites are then summarized. Emerging PSi advances in the context of cancer immunotherapy are also highlighted. Overall, very promising research results encourage further exploration of PSi for biomedical applications, particularly in drug delivery and cancer immunotherapy, and future translation of PSi into clinical applications.

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Simply and effectively control the shell thickness of hollow mesoporous silica nanoparticles by polyethylene glycol for drug delivery applications

Nguyen

Tran

Truong‐Thi

et al. 2022

J of Applied Polymer Sci

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This study reports for the first time the usage of polyethylene glycol (PEG) as the capping agent to control mesoporous shell thickness of hollow mesoporous silica nanoparticles (HMSN)—a promising nanocarrier. HMSN was synthesized with hard template method and PEG with different molecular weights and concentrations would be added at the mesoporous coating. The samples dSiO2@MSN synthesized with and without PEG were analyzed DLS, field‐emission scanning electron microscopy, Brunauer–Emmett–Teller and Barrett–Joyner–Halenda to characterize their mesoporous shells. Meanwhile transmission electron microscope, Zeta potential, energy dispersive X‐ray spectroscopy, Fourier‐transform infrared, loading capacity, release profile and cytotoxicity analysis was conducted to characterize HSMNs. As PEG's molecular weight or concentration increased, the mesoporous shell thickness gradually raised. Particles synthesized in the presence of 2% PEG 6000 retained the monodispersed spherical morphology with a particle diameter of 95.40 nm and a mesoporous shell thickness of 14.40 nm, which was about 7.0 nm thicker. dSiO2@MSN‐P owned equal mesopore diameter and higher surface area compared to dSiO2@MSN‐0. HMSN‐P showed similar loading capacity but better sustained release profile than HMSN‐0. Moreover, both HMSN‐0 and HMSN‐P performed as biocompatible materials. This study would contribute a simple and effective method to control the shell thickness of HMSN with PEG for drug delivery applications.

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Active Diffusion of Nanoparticles of Maternal Origin Within the Embryonic Brain

Abstract: PSi NPs penetrate deep in the brain tissues of embryos after intraventricular injection and after iv. injection to the mother.

Cited by 12 publications

References 48 publications

In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity

In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity

Tailoring Porous Silicon for Biomedical Applications: From Drug Delivery to Cancer Immunotherapy

Simply and effectively control the shell thickness of hollow mesoporous silica nanoparticles by polyethylene glycol for drug delivery applications

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