Active cop, passive cop: Developmental stage-specific modes of wound-induced blood cell recruitment in Drosophila

Brock, Amanda R.; Babcock, Daniel T.; Galko, Michael J.

doi:10.4161/fly.7395

Cited by 12 publications

(12 citation statements)

References 11 publications

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“…Fascin is expressed specifically in the motile subpopulation of embryonic hemocytes, the plasmatocytes. Furthermore, Fascin expression is eventually lost in plasmatocytes RESEARCH ARTICLE Development 136 (15) at larval stages (J.Z., unpublished), when these cells become immobile Brock et al, 2008), showing that Fascin is characteristic of motile populations of blood cells. Consistent with observations in cultured cells (Adams, 2004;Mattila and Lappalainen, 2008), we show that Fascin is enriched in filopodia-like cellular extensions, or microspikes, within the lamellipodia of migrating plasmatocytes in vivo.…”

Section: Fascin Controls In Vivo Cell Migrationmentioning

confidence: 99%

Fascin is required for blood cell migration during Drosophilaembryogenesis

et al. 2009

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Fascin is well characterized in vitro as an actin-bundling protein and its increased expression is correlated with the invasiveness of various cancers. However, the actual roles and regulation of Fascin in vivo remain elusive. Here we show that Fascin is required for the invasive-like migration of blood cells in Drosophila embryos. Fascin expression is highly regulated during embryonic development and, within the blood lineage, is specific to the motile subpopulation of cells, which comprises macrophage-like plasmatocytes. We show that Fascin is required for plasmatocyte migration, both as these cells undergo developmental dispersal and during an inflammatory response to epithelial wounding. Live analyses further demonstrate that Fascin localizes to, and is essential for the assembly of, dynamic actin-rich microspikes within plasmatocyte lamellae that polarize towards the direction of migration. We show that a regulatory serine of Fascin identified from in vitro studies is not required for in vivo cell motility, but is crucial for the formation of actin bundles within epithelial bristles. Together, these results offer a first glimpse into the mechanisms regulating Fascin function during normal development, which might be relevant for understanding the impact of Fascin in cancers.

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Section: Fascin Controls In Vivo Cell Migrationmentioning

confidence: 99%

Fascin is required for blood cell migration during Drosophilaembryogenesis

et al. 2009

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“…It is possible that the lesions in the epidermis of Dif dl larvae are the result of a developmental defect. Alternatively, because each animal has only one or a few epidermal lesions and their location varies, we prefer the hypothesis that epidermal gaps arise because of defective healing of wounds that occur normally in the life of the larva (Brock et al, 2008). In addition, Rel signaling in epidermal cells might help to prevent infection by local expression of products with antimicrobial activity (Onfelt Tingvall et al, 2001).…”

Section: Distinct Functions Of Rel Proteins In Blood Cells and The Epmentioning

confidence: 99%

DrosophilaRel proteins are central regulators of a robust, multi-organ immune network

Matova

Anderson

2010

Journal of Cell Science

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SummarySurvival of all animals depends on effective protection against infection. In Drosophila, opportunistic infection kills larvae if they lack the Rel/NF-B proteins Dorsal and Dif. We have used tissue-specific expression of Dif and Dorsal to reveal that these Rel proteins act in three different tissues to defend larvae from infection. Dif and Dorsal act in circulating blood cells, where they are required autonomously to promote blood-cell survival and phagocytosis of microorganisms. We show that a major transcriptional target of Dorsal and Dif in blood cells is Drosophila IAP1, a gene protecting these cells from death. We find that in addition to their autonomous role in blood-cell survival, Dif and Dorsal also act in the fat body to produce factors that promote blood-cell viability. These Rel proteins act in the epidermis to prevent infection by maintaining a barrier to microbial entry. Dorsal or Dif in any one of the three tissues is sufficient to defend the animal from opportunistic infection. Thus Drosophila has a multi-pronged system of defense and each branch of this network requires Rel proteins. Based on similarities between Drosophila and mammals, we propose that a Reldependent network is an ancient and robust framework of animal immune systems.

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“…Drosophila blood cells, or hemocytes, mediate innate immunity, removal of apoptotic cells, wound healing and secretion of extracellular matrix Lemaitre and Hoffmann, 2007;Wood and Jacinto, 2007;Brock et al, 2008;Dushay, 2009). Many molecular and cellular aspects of hemocyte development and responses are well conserved between Drosophila and vertebrates Hartenstein, 2006;Lemaitre and Hoffmann, 2007;Martinez-Agosto et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

The peripheral nervous system supports blood cell homing and survival in theDrosophilalarva

et al. 2011

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SUMMARYInteractions of hematopoietic cells with their microenvironment control blood cell colonization, homing and hematopoiesis. Here, we introduce larval hematopoiesis as the first Drosophila model for hematopoietic colonization and the role of the peripheral nervous system (PNS) as a microenvironment in hematopoiesis. The Drosophila larval hematopoietic system is founded by differentiated hemocytes of the embryo, which colonize segmentally repeated epidermal-muscular pockets and proliferate in these locations. Importantly, we show that these resident hemocytes tightly colocalize with peripheral neurons and we demonstrate that larval hemocytes depend on the PNS as an attractive and trophic microenvironment. atonal (ato) mutant or genetically ablated larvae, which are deficient for subsets of peripheral neurons, show a progressive apoptotic decline in hemocytes and an incomplete resident hemocyte pattern, whereas supernumerary peripheral neurons induced by ectopic expression of the proneural gene scute (sc) misdirect hemocytes to these ectopic locations. This PNS-hematopoietic connection in Drosophila parallels the emerging role of the PNS in hematopoiesis and immune functions in vertebrates, and provides the basis for the systematic genetic dissection of the PNS-hematopoietic axis in the future.

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Active cop, passive cop: Developmental stage-specific modes of wound-induced blood cell recruitment in Drosophila

Cited by 12 publications

References 11 publications

Fascin is required for blood cell migration during Drosophilaembryogenesis

Fascin is required for blood cell migration during Drosophilaembryogenesis

DrosophilaRel proteins are central regulators of a robust, multi-organ immune network

The peripheral nervous system supports blood cell homing and survival in theDrosophilalarva

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