Active compounds from Baoyuan decoction with myocardial protection activities and their potential targets

Abstract

Baoyuan decoction (BYD) is a classical Chinese formula for coronary heart disease with Qi deficiency, blood stasis tonifying Qi and Yang deficiency. However, the chemical material basis and underlying action mechanisms of BYD still lack systemic study. In order to clarify the active compounds and the potential action mechanisms of BYD, the oxygen-glucose deprivation/recovery (OGD/R)-induced H9c2 cells injury models was used to screen the monomeric compounds of BYD with myocardial protection activity. PubChem's BioAssay database was then used to analyze the potential targets of active monomeric compounds, classify the predicted biological targets, and analyze the internal relation between active compounds of BYD and biological targets. The screening results showed that BYD aqueous extract and 17 monomeric compounds could significantly increase the survival rate of OGD/R-induced H9c2 myocardial cells. The results of virtual targets screening study showed that 15 monomeric compounds and the potential mechanisms for myocardial protection were related to oxidative stress pathway, calcium ion pathway, mitochondrial protection, anti-apoptosis, etc. These results verified that BYD had myocardial protection effect, which was obtained by network regulation of multi-components and multi-targets. All these results provide the theoretical basis and references for the clinical usage of BYD in treatment of coronary heart disease.

Integration of Metabonomics and Transcriptomics Reveals the Therapeutic Effects and Mechanisms of Baoyuan Decoction for Myocardial Ischemia

“…The present study and our previous researches ( Shu et al, 2016 ; Yu et al, 2016 , 2017 ; Wan et al, 2017 ) have proved that BYD and the active ingredients therein have multiple potential therapeutic properties against MI. Of particularly, the multi-omics results indicated that BYD could significantly regulate transcripts encoded ETC complexes and reverse the energy substrates and the oxidative stress related-metabolites, which were further supported by the pharmacodynamics effects of promoting the cardiac ATP generation and enhancing the serum antioxidation capacity.…”

“…We used an OGD/R-induced cardiomyocyte apoptosis model to evaluate the protection effect of BYD and to search for its active components, and the results showed that BYD and 17 active single components have the anti-apoptosis activities, and the virtual targets screening of the active components by the PubChem BioAssay database showed that the potentially cardioprotective mechanisms are related to oxidative stress pathway, mitochondrial protection, anti-apoptosis, etc. ( Shu et al, 2016 ).…”

“…The main components found in BYD were disclosed to be ginsenosides, astragalosides, licorice saponins, and flavonoids ( Ma et al, 2015 , 2016 , 2017 ). Notwithstanding that many active compounds have been identified from BYD and some of their potential effects in relation to the treatment of CVDs have been disclosed ( Shu et al, 2016 ; Wan et al, 2017 ; Zhang et al, 2017 ), there still exists a challenge in clarification of the action mechanisms of BYD because of the complex interaction of its multi-components.…”

BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway

“…The extraction method was performed as reported previously ( Ma et al, 2016 ). The fingerprint of BYD was analyzed by high-performance liquid chromatography (HPLC), and the typical chromatogram was shown in a previous study ( Shu et al, 2016 ).…”

Comparative study on the main active components of Baoyuan decoction in plasma and urine of normal and heart failure rats

“…So far, 276 compounds have been identified in BYD, mainly including flavonoid and saponin compounds (Ma et al, 2015(Ma et al, , 2016(Ma et al, , 2017Sun et al, 2010). Some preliminary studies had shown that astragaloside, ginsenoside Re, ginsenoside Rg1, formononetin, and other bioactive ingredients possessed myocardial protective effects (Du et al, 2020;Shu et al, 2016).…”