Active Cl? absorption by the Chinese crab (Eriocheir sinensis) gill epithelium measured by transepithelial potential difference

Roles of crustacean hyperglycaemic hormone in ionic and metabolic homeostasis in the Christmas Island Blue crabDiscoplax celeste

“…This was despite the fact that Na + /K + -ATPase has previously been shown to be instrumental in driving ion uptake at the gills in G. natalis (Morris and Ahern, 2003) as well as in other terrestrial crabs, including Birgus latro (Morris et al, 2000;Greenaway, 2003). It is well established that it is V-ATPase that plays a significant role in ion regulation in the gills of freshwater crustaceans, including the freshwater crayfish Cherax destructor (Onken and Graszynski, 1989), E. sinensis (Onken et al, 1991;Onken and Putzenlechner, 1995;Riestenpatt et al, 1995) and the South American true freshwater crab Dilocarcinus pagei (Weihrauch et al, 2004). However, further investigations in the present study were unable to show any significant effect of CHH on V-ATPase activity in D. celeste (supplementary material Fig.S2), thus indicating that the mode of action of CHH on the gills to facilitate ion uptake remains to be fully characterised in terrestrial crabs.…”

“…Whilst significant progress in understanding crustacean branchial membrane ionic transport processes have been made, by utilising euryhaline crab models such as Eriocheir sinensis (Onken and Graszynski, 1989;Trausch et al, 1989;Bianchini and Gilles, 1990;Detaille et al, 1992;Riestenpatt et al, 1994;Onken and Putzenlechner, 1995;Riestenpatt et al, 1995;Mo et al, 1998;Onken, 1999;Onken et al, 2000;Mo et al, 2003), our approach in investigating possible roles for CHH involved a rather less well known terrestrial/amphibious gecarcinid, the Christmas Island blue crab, Discoplax celeste (Ng and Davie, 2012). We reasoned that this might be a useful model, because as with all terrestrial crabs, conservation of water and salts occurs via reprocessing of primary urine from the antennal glands over the gills, to produce a hypoionic final urine 'P' (Wolcott and Wolcott, 1985), with less than 30% of the ions measured in the primary urine and 5% of ions found in the haemolymph (Morris, 2001).…”

The transepithelial voltage of the isolated anterior stomach of mosquito larvae (Aedes aegypti): pharmacological characterization of the serotonin-stimulated cells

“…A similar approach has been successfully used to analyze the transport mechanisms of NaCl absorption across the gills of hyperosmoregulating crabs (Drews and Graszynski, 1987;Onken and Graszynski, 1989). Nevertheless, it can be argued that the large scatter of the Vte before and after stimulation with serotonin (see Results) is related to the experimental approach.…”

Osmoregulation and immunolocalization of Na+/K+-ATPase during the ontogeny of the mitten crab Eriocheir sinensis (Decapoda, Grapsoidea)

“…It has become a model for studies of gill morphology (Barra et al 1983) and physiology (Riestenpatt et al 1994), in particular in relation to ionic regulation and excretion (Péqueux & Gilles 1981, Rathmeyer & Siebers 2001, electrophysiology (Onken & Graszynski 1989, Onken et al 1991, and enzyme activities in gills (Olsowski et al 1995, Mo et al 1998). E. sinensis has received much attention also as an invasive species.…”