Active Capture and Transport of Virus Particles Using a Biomolecular Motor‐Driven, Nanoscale Antibody Sandwich Assay

Bachand, George D.; Rivera, Susan B.; Carroll‐Portillo, Amanda; Hess, Henry; Bachand, Marlene

doi:10.1002/smll.200500262

Cited by 116 publications

(107 citation statements)

References 36 publications

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“…Following the first demonstration of HMM driven transportation of actin filaments with quantum dots attached to the actin monomers via biotin–streptavidin (Månsson et al 2004) and similar studies for kinesin-propelled microtubules (Bachand et al 2004), extensive developments have followed with main focus on microtubule–kinesin (Bachand et al 2004, 2006; Carroll-Portillo et al 2009a, b; Diez et al 2003; Hiyama et al 2009, 2010; Hutchins et al 2007; Malcos and Hancock 2011; Raab and Hancock 2008; Ramachandran et al 2006; Rios and Bachand 2009). The considerably fewer numbers of studies with actomyosin in focus may have different reasons (see Introduction) but it is generally believed that it is considerably more difficult to achieve transportation using HMM propelled actin filaments as shuttles.…”

Section: Developments From 2005 and Onwardsmentioning

confidence: 99%

Translational actomyosin research: fundamental insights and applications hand in hand

Månsson

2012

J Muscle Res Cell Motil

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This review describes the development towards actomyosin based nanodevices taking a starting point in pioneering studies in the 1990s based on conventional in vitro motility assays. References are given to parallel developments using the kinesin–microtubule motor system. The early developments focused on achieving cargo-transportation using actin filaments as cargo-loaded shuttles propelled by surface-adsorbed heavy meromyosin along micro- and nanofabricated channels. These efforts prompted extensive studies of surface–motor interactions contributing with new insights of general relevance in surface and colloid chemistry. As a result of these early efforts, a range of complex devices have now emerged, spanning applications in medical diagnostics, biocomputation and formation of complex nanostructures by self-organization. In addition to giving a comprehensive account of the developments towards real-world applications an important goal of the present review is to demonstrate important connections between the applied studies and fundamental biophysical studies of actomyosin and muscle function. Thus the manipulation of the motor proteins towards applications has resulted in new insights into methodological aspects of the in vitro motiliy assay. Other developments have advanced the understanding of the dynamic materials properties of actin filaments.

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Section: Developments From 2005 and Onwardsmentioning

confidence: 99%

Translational actomyosin research: fundamental insights and applications hand in hand

Månsson

2012

J Muscle Res Cell Motil

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“…In this arrangement, cargoes such as nano-or microspheres [7,14,41,40], virus particles [8], proteins [81,55,23], DNAs [16,91,80] and lipid vesicles [46] have been successfully transported. Considering that the gliding direction of MTs can be controlled by utilizing preconfigured microlithographic tracks [39,47] or by applying electric [95] and magnetic fields [48], the main challenge in this arrangement is to load/unload cargoes onto/from gliding MTs at a sender/receiver in a reversible manner.…”

Section: Active Transportmentioning

confidence: 98%

Molecular communication: Harnessing biochemical materials to engineer biomimetic communication systems

Hiyama¹,

Moritani²

2010

Nano Communication Networks

116

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“…To preclude this possibility, microtubules have been directly functionalized with antibodies through covalent crosslinking (Bachand et al 2006; Soto et al 2008; Carroll-Portillo et al 2009). To maximize function, antibodies can be bound to microtubules in a preferred orientation (e.g., through the F c domain), which orients the antigen-binding F ab region away from the microtubule surface (Bachand et al 2009).…”

Section: Functionalizing Microtubules With Antibodiesmentioning

confidence: 99%

Engineering tubulin: microtubule functionalization approaches for nanoscale device applications

Malcos

Hancock

2011

Appl Microbiol Biotechnol

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With the emergences of engineered devices at microscale and nanoscale dimensions, there is a growing need for controlled actuation and transport at these length scales. The kinesin–microtubule system provides a highly evolved biological transport system well suited for these tasks. Accordingly, there is an ongoing effort to create hybrid nanodevices that integrate biological components with engineered materials for applications such as biological separations, nanoscale assembly, and sensing. Adopting microtubules for these applications generally requires covalent attachment of biotin, fluorophores, or other biomolecules to tubulin enable surface or cargo attachment, or visualization. This review summarizes different strategies for functionalizing microtubules for application-focused as well as basic biological research. These functionalization strategies must maintain the integrity of microtubule proteins so that they do not depolymerize and can be transported by kinesin motors, while adding utility such as the ability to reversibly bind cargo. The relevant biochemical and electrical properties of microtubules are discussed, as well as strategies for microtubule stabilization and long-term storage. Next, attachment strategies, such as antibodies and DNA hybridization that have proven useful to date, are discussed in the context of ongoing hybrid nanodevice research. The review concludes with a discussion of less explored opportunities, such as harnessing the utility of tubulin posttranslational modifications and the use of recombinant tubulin that may enable future progress in nanodevice development.

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Active Capture and Transport of Virus Particles Using a Biomolecular Motor‐Driven, Nanoscale Antibody Sandwich Assay

Cited by 116 publications

References 36 publications

Translational actomyosin research: fundamental insights and applications hand in hand

Translational actomyosin research: fundamental insights and applications hand in hand

Molecular communication: Harnessing biochemical materials to engineer biomimetic communication systems

Engineering tubulin: microtubule functionalization approaches for nanoscale device applications

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