Active bioparticle manipulation in microfluidic systems

Ali, Mohd Alaudin Mohd; Ostrikov, Kostya Ken; Khalid, Fararishah Abdul; Majlis, Burhanuddin Yeop; Kayani, Aminuddin Bin Ahmad

doi:10.1039/c6ra20080j

Cited by 28 publications

(13 citation statements)

References 222 publications

(367 reference statements)

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“…These forces include electrophoresis, 46 dielectrophoresis, 47 magnetophoresis, 48 optical tweezing, 49 thermophoresis, 50 and acoustophoresis, 51 and they can be incorporated into microfluidic platforms for particle manipulation. The fundamentals and a brief description of each BMF were covered in a recent review by Ali et al 12 The selection of a suitable BMF for cancer or CTC diagnostic devices must take into account the physical properties of cancer cells. Some physical properties of cancer cells include an average cell diameter of 10.3-32.39 lm, [52][53][54][55] they are electrically neutral, 56 and they are able to preserve cell viability and cell purity for downstream analysis.…”

Section: Cancer Tumor and Cancer Diagnostic Devicesmentioning

confidence: 99%

“…56 Magnetophoresis requires complex preparations of a conductive suspending medium, which complicates the purification procedure and may limit the availability of the target cells. 12,60 Meanwhile, optical tweezing requires complicated setup procedures and instruments, 61 while acoustophoresis faces the challenge of chaotic streaming flow from acoustic waves 62 inside a complex microfluidic channel. 63 In dielectrophoresis (DEP), particles are temporarily polarized, by a spatially non-uniform electric field, 64 establishing dipoles which induce unequal columbic forces causing the particles to move.…”

Section: Cancer Tumor and Cancer Diagnostic Devicesmentioning

confidence: 99%

“…The external forces applied to control and manipulate biological particles (bioparticles) in a microfluidic platform are known as bioparticle active manipulation forces. 12 Dielectrophoresis (DEP) is one of the forces widely applied to cells in microfluidic platforms to control their motions. Dielectrophoresis (DEP) is an electro-kinetic phenomenon describing the motion of a neutral particle or cell in a non-uniform electric field due to polarization effects.…”

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confidence: 99%

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Dielectrophoresis-based microfluidic platforms for cancer diagnostics

et al. 2018

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The recent advancement of dielectrophoresis (DEP)-enabled microfluidic platforms is opening new opportunities for potential use in cancer disease diagnostics. DEP is advantageous because of its specificity, low cost, small sample volume requirement, and tuneable property for microfluidic platforms. These intrinsic advantages have made it especially suitable for developing microfluidic cancer diagnostic platforms. This review focuses on a comprehensive analysis of the recent developments of DEP enabled microfluidic platforms sorted according to the target cancer cell. Each study is critically analyzed, and the features of each platform, the performance, added functionality for clinical use, and the types of samples, used are discussed. We address the novelty of the techniques, strategies, and design configuration used in improving on existing technologies or previous studies. A summary of comparing the developmental extent of each study is made, and we conclude with a treatment of future trends and a brief summary.

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Section: Cancer Tumor and Cancer Diagnostic Devicesmentioning

confidence: 99%

Section: Cancer Tumor and Cancer Diagnostic Devicesmentioning

confidence: 99%

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confidence: 99%

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Dielectrophoresis-based microfluidic platforms for cancer diagnostics

et al. 2018

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“…DEP is a process of manipulating particle movement using non-uniform electric fields and it has wide bio-particle applications [18,19,20,21,22]. The force, magnitude, and direction of DEP are regulated by the relative particle polarization; therefore, the particles experience a force that either attracts the particles in the direction of the high electric field gradient region or a force that causes the particles to be repelled from those regions [23].…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis of Amyloid-Beta Proteins as a Microfluidic Template for Alzheimer’s Research

Al-Ahdal

Kayani

Ali

et al. 2019

IJMS

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We employed dielectrophoresis to a yeast cell suspension containing amyloid-beta proteins (Aβ) in a microfluidic environment. The Aβ was separated from the cells and characterized using the gradual dissolution of Aβ as a function of the applied dielectrophoretic parameters. We established the gradual dissolution of Aβ under specific dielectrophoretic parameters. Further, Aβ in the fibril form at the tip of the electrode dissolved at high frequency. This was perhaps due to the conductivity of the suspending medium changing according to the frequency, which resulted in a higher temperature at the tips of the electrodes, and consequently in the breakdown of the hydrogen bonds. However, those shaped as spheroidal monomers experienced a delay in the Aβ fibril transformation process. Yeast cells exposed to relatively low temperatures at the base of the electrode did not experience a positive or negative change in viability. The DEP microfluidic platform incorporating the integrated microtip electrode array was able to selectively manipulate the yeast cells and dissolve the Aβ to a controlled extent. We demonstrate suitable dielectrophoretic parameters to induce such manipulation, which is highly relevant for Aβ-related colloidal microfluidic research and could be applied to Alzheimer’s research in the future.

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“…Microfluidic systems, which have networks of microchannels that have dimensions ranging from tenths to hundredths of micrometers, are used to manipulate picoliter-level laminar fluid flow, discrete fluid droplets, particles, or cells [1,2,3,4]. Their functional, analytical, and sampling features are attractive for application in biology, life sciences (such as for cytometry), diagnostics lab-on-chip devices, microscale cell culture, etc.…”

Section: Introductionmentioning

confidence: 99%

Active PZT Composite Microfluidic Channel for Bioparticle Manipulation

Janušas

Pilkauskas

Palevičius

2019

Sensors

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The concept of active microchannel for precise manipulation of particles in biomedicine is reported in this paper. A novel vibration-assisted thermal imprint method is proposed for effective formation of a microchannel network in the nanocomposite piezo polymer layer. In this method, bulk acoustic waves of different wavelengths excited in an imprinted microstructure enable it to function in trapping–patterning, valve, or free particle passing modes. Acoustic waves are excited using a special pattern of electrodes formed on its top surface and a single electric ground electrode formed on the bottom surface. To develop the microchannel, we first started with lead zirconate titanate (PZT) nanopowder [Pb (Zrx, Ti1–x) O3] synthesis. The PZT was further mixed with three different binding materials—polyvinyl butyral (PVB), poly(methyl methacrylate) (PMMA), and polystyrene (PS)—in benzyl alcohol to prepare a screen-printing paste. Then, using conventional screen printing techniques, three types of PZT coatings on copper foil substrates were obtained. To improve the voltage characteristics, the coatings were polarized. Their structural and chemical composition was analyzed using scanning electron microscope (SEM), while the mechanical and electrical characteristics were determined using the COMSOL Multiphysics model with experimentally obtained parameters of periodic response of the layered copper foil structure. The hydrophobic properties of the PZT composite were analyzed by measuring the contact angle between the distilled water drop and the three different polymer composites: PZT with PVB, PZT with PMMA, and PZT with PS. Finally, the behavior of the microchannel formed in the nanocomposite piezo polymer was simulated by applying electrical excitation signal on the pattern of electrodes and then analyzed experimentally using holographic interferometry. Wave-shaped vibration forms of the microchannel were obtained, thereby enabling particle manipulation.

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Active bioparticle manipulation in microfluidic systems

Abstract: The motion of bioparticles in a microfluidic environment can be actively controlled using several tuneable mechanisms, including hydrodynamic, electrophoresis, dielectrophoresis, magnetophoresis, acoustophoresis, thermophoresis and optical forces.

Cited by 28 publications

References 222 publications

Dielectrophoresis-based microfluidic platforms for cancer diagnostics

Dielectrophoresis-based microfluidic platforms for cancer diagnostics

Dielectrophoresis of Amyloid-Beta Proteins as a Microfluidic Template for Alzheimer’s Research

Active PZT Composite Microfluidic Channel for Bioparticle Manipulation

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