Activation state of muscle satellite cells isolated from steers implanted with a combined trenbolone acetate and estradiol implant.

Johnson, B. J.; Halstead, N; White, Michael E.; Hathaway, Marcia; DiCostanzo, A.; Dayton, William R

doi:10.2527/1998.76112779x

Cited by 91 publications

(73 citation statements)

References 26 publications

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“…This difference may be due to either a difference in plating efficiency/cell survival within the first days after seeding the cells or may be an indication of the SCs responding at a different rate to seeding and medium -giving rise to a lag period before proliferation starts. A lag phase has been observed by others, with a difference in the length of the lag phase dependent on whether or not cattle were implanted with a trenbolone acetate/estradiol implant before isolation of SC (Johnson et al, 1998). Also SCs isolated from rats at different ages exhibit various lengths of the lag period before proliferation starts (Schultz and Lipton, 1982).…”

Section: Discussionmentioning

confidence: 92%

In vitro primary satellite cell growth and differentiation within litters of pigs

Nissen

Oksbjerg

2009

Animal

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Postnatal muscle growth is dependent on satellite cell (SC) proliferation, differentiation and fusion to increase the DNA content of existing muscle fibres and thereby the capacity to synthesize protein. The purpose of the present study was to examine the ability of isolated SCs from low, medium and high weaning weight litter mates of pigs to proliferate and differentiate, and to affect protein synthesis and degradation after fusion into myotubes. At 6 weeks of age, SCs from the lowest weight (LW), medium weight (MW) and highest weight (HW) female pigs within eight litters were isolated. Thereby, eight cultures of SCs were established for each of the three weight groups within litter, representing three groups of SCs from pigs exhibiting differences in postnatal muscle growth performance. Proliferation was estimated as the number of viable cells at different time points after seeding. SC differentiation was evaluated by measuring the activity of the muscle-specific enzyme, creatine phosphokinase, and protein synthesis and degradation were measured by incorporation and release of 3 H-tyrosine, respectively. A tendency towards a difference in proliferation between SC cultures was found ( P 5 0.09). This was evident as the number of viable cells at day 3 was lower in cultures from LW pigs than from HW ( P , 0.05) and MW ( P , 0.01) pigs. Differentiation was significantly different between cultures ( P , 0.05). There was a significant difference between LW and MW cultures at 72 h ( P , 0.05), and a tendency towards a difference between LW and HW cultures at 45 h ( P 5 0.07). Protein synthesis per mg protein or per mg DNA did not differ among SC cultures from LW, MW and HW pigs. Neither did protein degradation rate differ significantly among SC cultures from LW, MW and HW pigs. Overall, the results show that SCs from LW pigs seem to proliferate and differentiate at a slower rate than SCs from MW and HW pigs. The results found in this study show no difference in the ability of SCs to affect protein synthesis or degradation between SCs from litter mates exhibiting different growth rates in vivo.

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Section: Discussionmentioning

confidence: 92%

In vitro primary satellite cell growth and differentiation within litters of pigs

Nissen

Oksbjerg

2009

Animal

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“…Hormone implants are considered the single best management tool to improve feed efficiency and average daily gain in beef cattle (Wileman, 2009;Hermesmeyer, 2000;Johnson, 1998;Lawrence, 2007). Hormones were first researched in food animal production systems in 1947 with the advent of diethylestilbesterol (DES) and have been FDA approved for use orally in beef cattle production since 1954 (Raun, 2002) to increase feed utilization, stimulate growth, and increase leanness of the animal.…”

Section: Hormone Implants In Feedlot Cattlementioning

confidence: 99%

“…It is imperative to improve cattle growth efficiency and decrease the time to get animals to market. One particular technology, hormone implants, have been FDA approved for use in beef cattle production since 1957 (Raun, 2002) and are considered the single best management tool to improve feed efficiency and average daily gain in beef cattle (Wileman, 2009;Hermesmeyer, 2000;Johnson, 1998;Lawrence, 2007). The use of hormonal implants in cattle improve ADG, DMI, HCW and lower feed-to-gain ratios when compared to non-implanted cattle (Reinhardt, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effects of delayed steroid implanting on health, performance, and carcass quality in high health risk, auction market-sourced feedlot steers1,2

Munson

Thomson

Reinhardt

2012

Journal of Animal Science

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Auction derived feeder calves (n=1,601; initial BW = 273.5 + 4.7 kg) were used to examine the effects of delayed administration of the initial steroid implant on health, performance, and carcass characteristics of feedlot cattle. Steers were procured from multiple- were obtained by USDA personnel; presence of lung lesions, pleural adhesions, and liver abscesses was evaluated by trained university personnel. Delaying the initial implant tended to reduce morbidity (24.7 vs. 28.5%; P = 0.13) and reduced railer rates (1.8 vs. 3.3%; P = 0.02); however, there were no effects of timing of implant administration (P ≥ 0.31) on rates of retreatment, mortality, lung lesions, or pleural adhesions. Implanting immediately upon feedlot arrival resulted in numerical improvements in ADG and feed conversion, but these differences were not statistical (P ≥ 0.56). Cattle implanted upon arrival had numerically greater HCW and yield grade vs. cattle implanted on d 45; however, these differences were not statistical (P ≥ 0.16). Delaying the initial implant 45 d did not influence animal health, performance parameters or carcass characteristics in high risk feeder calves.v

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“…Androgenic and estrogenic implants are widely used, both individually and in combination, as growth promoters for cattle (Hayden et al, 1993;Johnson et al, 1996). Also, it was shown that artificial implantation of growth hormones increased the number of satellite cells (Johnson et al, 1998). Additionally, a number of studies in humans and animals have shown that hormone treatment increases the muscle fiber diameter.…”

Section: Introductionmentioning

confidence: 99%

Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition

Rajesh¹,

Kim²,

Park³

et al. 2009

Journal of Animal Science and Technology

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The aim of this study was to analyze the proteome of proliferating bovine satellite cells from longissimus dorsi, deep pectoral and semitendinosus muscle depots which had been subjected to hormonal deprivation or addition in culture. For hormone deprivation or addition studies, the cells were either grown in 10% charcoal-dextran stripped fetal bovine serum (CD-FBS) or in 10% FBS supplemented medium. Further to analyze the effect of insulin like growth factor (IGF-1) and testosterone (TS), the cells were grown in 10% CD-FBS containing IGF-1 (10 ng/ml) or TS (10 nM). Results have shown that hormone deprivation had a negative impact on proliferation of the cells from each of the muscle depots. In case of IGF-1 and TS addition, the proliferation levels were low compared with that of the cells grown in 10% FBS. Hence, to gain the insights of the proteins that are involved in such divergent levels of proliferation, the proteome of such satellite cells proliferating under the above mentioned conditions were analyzed using 2D-DIGE and MALDI-ToF/ToF. Thirteen proteins during hormone deprivation and nine proteins from hormone addition were found to be differentially expressed in all the cultures of the cells from the three depots. Moreover, the results highlighted in this study offer a role for each differentially expressed protein with respect to its effect on positive or negative regulation of cell proliferation.

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Activation state of muscle satellite cells isolated from steers implanted with a combined trenbolone acetate and estradiol implant.

Cited by 91 publications

References 26 publications

In vitro primary satellite cell growth and differentiation within litters of pigs

In vitro primary satellite cell growth and differentiation within litters of pigs

Effects of delayed steroid implanting on health, performance, and carcass quality in high health risk, auction market-sourced feedlot steers1,2

Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition

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