Activation of γδ T Cells in the Primary Immune Response to
            <i>Mycobacterium tuberculosis</i>

Janis, Eric M.; Kaufmann, Stefan H. E.; Schwartz, Ronald H.; Pardoll, Drew M.

doi:10.1126/science.2524098

Cited by 443 publications

(185 citation statements)

References 39 publications

(6 reference statements)

Supporting

Mentioning

175

Contrasting

Unclassified

Order By: Relevance

“…The results presented here strongly suggest that human BB3/TiyA + T cells recognize different determinants than non-MHC-restricted NK cells (3,4) or IL-2-activated MHC-restricted a/o T cells (31). The relationship of these target structures to known antigens for y/S T cells remains to be determined (12)(13)(14)32). However, these target structures cannot be related to HLA class I since Daudi cells do not express 02m-associated determinants .…”

Section: Resultsmentioning

confidence: 85%

Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis.

Fisch

Malkovsky

Braakman

et al. 1990

The Journal of Experimental Medicine

159

View full text Add to dashboard Cite

Non-MHC-restricted killer cells are cytotoxic lymphocytes that can mediate cytolysis of most tumor targets without apparent selectivity and restriction by the MHC, particularly when activated with IL-2. These effector cells include predominantly NK cells and T cells expressing the TCR-gamma/delta. We found that TCR-gamma/delta-1+, delta TSC1-, BB3+, Ti gamma A+ T cell clones mediate a characteristic cytolytic pattern of non-MHC-restricted cytolysis that is markedly different from NK clones and alpha/beta T cell clones derived from the peripheral blood of the same normal individuals. The characteristic finding is that all BB3/Ti gamma A+ gamma/delta clones mediate strong cytolysis of Daudi cells but they do not lyse Raji cells. In contrast, NK clones from the same donors mediate strong cytolysis of both Daudi and Raji targets. Cytotoxicity by the gamma/delta clones on certain target cells such as Daudi and Molt 4 can be specifically inhibited by mAbs reactive against the TCR-gamma/delta. Therefore, the TCR-gamma/delta on these clones either directly recognizes target epitopes on some tumor targets or it is involved in the regulation of their cytotoxic function. The expression of TCR-gamma/delta products reacting with the BB3 and Ti gamma A mAbs reflects the usage of identical TCR-gamma/delta V region genes that appear to be associated with the characteristic pattern of non-MHC-restricted cytotoxicity displayed by this major subset of human peripheral blood gamma/delta cells.

show abstract

Section: Resultsmentioning

confidence: 85%

Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis.

Fisch

Malkovsky

Braakman

et al. 1990

The Journal of Experimental Medicine

159

View full text Add to dashboard Cite

show abstract

“…Most of these studies are related to infections such as Listeria monocytogenes, Salmonella choleraesuis, Mycobacterium tuberculosis, M. leprae, influenza virus, and Candida albicans (15)(16)(17)(18)(19)(20)(21)(22)(23)(24). In these circumstances it is not always clear whether ␥␦ T cell Ag receptors are recognizing foreign Ags or interacting with self-ligands whose expression is induced by infection.…”

Section: Discussionmentioning

confidence: 99%

Infiltration of Canonical Vγ4/Vδ1 γδ T Cells in an Adriamycin-Induced Progressive Renal Failure Model

Ando

Watson

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

We have previously reported an infiltration of renal interstitial γδ T cells in Adriamycin-induced progressive glomerulosclerosis in the rat kidney. The TCR repertoire and sequences used by these γδ T cells have now been studied. Two injections of Adriamycin 14 days apart caused segmental glomerulosclerosis, massive interstitial infiltration of mononuclear cells, and end-stage renal failure. Flow cytometry of lymphocyte subpopulations with Abs to CD3, the γδ TCR, and the αβ TCR showed that γδ T cells as a proportion of CD3+ cells were increased in Adriamycin-treated kidneys (8.5 ± 5.4%), but not in lymph nodes (1.3 ± 0.4%). A semiquantitative score of glomerular damage (r = 0.65; p < 0.01) and creatinine (r = 0.62; p < 0.01) correlated significantly with the presence of γδ T cells. TCR Vγ repertoire analysis by RT-PCR and Southern blotting showed that Vγ2 was the dominant subfamily in lymph nodes, whereas Vγ4 became the predominant subfamily in advanced stages of the rat Adriamycin-treated kidney. Sequencing of the Vγ4-Jγ junctional region showed an invariant sequence. The amino acid sequence of the junctional region of the Vγ4 TCR was the same as the reported mouse canonical Vγ4 TCR sequence. Analysis of the kidney Vδ repertoire showed dominant expression of Vδ1, and sequencing again revealed the selective expression of a canonical Vδ1 gene. Semiquantitative RT-PCR for cytokine gene expression showed that γδ T cells from the kidneys expressed TGF-β, but not IL-4, IL-10, or IFN-γ. These results suggest that the predominant γδ T cells in the Adriamycin kidney use an invariant Vγ4/Vδ1 receptor.

show abstract

“…They differ from ␣␤-T cells in several parameters, including ontogenic appearance, tissue distribution, and Ag recognition (8). Although increased numbers of ␥␦-T lymphocytes have been observed in a number of infectious diseases (9,10), the physiologic and pathophysisologic roles of the cells in these disorders are not fully understood. There is evidence showing that ␥␦-T cells accumulate during the fibrogenic inflammation underlying wound healing (11) and in human infectious disease lesions (9).…”

mentioning

confidence: 99%

Human γδ-T Lymphocytes Express and Synthesize Connective Tissue Growth Factor: Effect of IL-15 and TGF-β1 and Comparison with αβ-T Lymphocytes

Workalemahu

Foerster

Kroegel

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

T lymphocytes bearing the γδ-TCR accumulate during wound healing and inflammation. However, the role of γδ-T lymphocytes in fibrogenic tissue reactions is not well understood. Therefore, we addressed the question of whether human γδ-T cells express and synthesize connective tissue growth factor (CTGF), a factor known to regulate fibrogenesis and wound healing. In addition, the lymphoblastic leukemia T cell line (Loucy) that possesses characteristics typical of γδ-T cells was used as a model to evaluate the regulation of CTGF gene expression. Blood γδ-T cells isolated from healthy donors were grown in the presence of IL-15/TGF-β1 for 48 h and assessed for the expression and synthesis of CTGF. Nonstimulated human blood γδ-T cells and Loucy γδ-T cells expressed low levels of CTGF mRNA. Costimulation of the cells with IL-15 and TGF-β1 resulted in a substantially increased level of CTGF mRNA expression within 4–8 h, and it remained elevated for at least 48 h. In contrast, no CTGF mRNA was detected when nonstimulated and stimulated human CD4+ αβ-T cells were analyzed. In addition, Western blot analysis of human γδ-T cell lysates prepared 4 days following stimulation with IL-15 and TGF-β1 revealed a 38-kDa CTGF protein in cell lysates of human γδ-T cells. Detection was confirmed using Colo 849 fibroblasts, which can constitutively express high levels of CTGF. In conclusion, we herein present novel evidence that in contrast to CD4+ αβ-T cells human γδ-T cells are capable of expressing CTGF mRNA and synthesizing its corresponding protein, which supports the concept that γδ-T cells may contribute to wound healing or tissue fibrotic processes.

show abstract

Activation of γδ T Cells in the Primary Immune Response to Mycobacterium tuberculosis

Cited by 443 publications

References 39 publications

Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis.

Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis.

Infiltration of Canonical Vγ4/Vδ1 γδ T Cells in an Adriamycin-Induced Progressive Renal Failure Model

Human γδ-T Lymphocytes Express and Synthesize Connective Tissue Growth Factor: Effect of IL-15 and TGF-β1 and Comparison with αβ-T Lymphocytes

Contact Info

Product

Resources

About