Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism

Abstract

Research has proven that light treatment, specifically red light radiation, can provide more clinical benefits to human health. Our investigation was firstly conducted to characterize the tissue morphology of mouse breast post 660 nm laser radiation with low power and long-term exposure. RNA sequencing results revealed that light exposure with a higher intervention dosage could cause a number of differentially expressed genes compared with a low intervention dosage. Gene ontology analysis, protein–protein interaction network analysis, and gene set enrichment analysis results suggested that 660 nm light exposure can activate more transcription-related pathways in HC11 breast epithelial cells, and these pathways may involve modulating critical gene expression. To consider the critical role of the Wnt/T-catenin pathway in light-induced modulation, we hypothesized that this pathway might play a major role in response to 660 nm light exposure. To validate our hypothesis, we conducted qRT-PCR, immunofluorescence staining, and Western blot assays, and relative results corroborated that laser radiation could promote expression levels of β-catenin and relative phosphorylation. Significant changes in metabolites and pathway analysis revealed that 660 nm laser could affect nucleotide metabolism by regulating purine metabolism. These findings suggest that the Wnt/β-catenin pathway may be the major sensor for 660 nm laser radiation, and it may be helpful to rescue drawbacks or side effects of 660 nm light exposure through relative interventional agents.

Introducing Critical Genes in Response to Photodynamic Therapy: A Network Analysis

“…Hubbottlenecks are critical elements of a network. [10][11][12] In the present study, the molecular mechanism of human cells response to PDT was investigated by data from GEO via a bioinformatics approach to open a new window about the main targets of the applied therapeutic method.…”

Long and Short-terms Effects of Ablative Fractional Laser Therapy on Human Skin: A Network Analysis

“…8 Xiao et al have studied the gene expression changes of the treated sample after laser therapy by using bioinformatics. 9 Protein-protein interaction (PPI) network analysis as a bioinformatics trend is used to assess the laser effect on the body. Rezaei-Tavirani et al have studied the effect of erbium: yttrium-aluminum-garnet laser irradiation on the skin via PPI network analysis.…”