Activation of Thymus Cells by Histocompatibility Antigens

Sprent, J.; Miller, J. F. A. P.

doi:10.1038/newbio234195a0

Cited by 89 publications

(58 citation statements)

References 5 publications

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“…Cells of both the murine mastocytoma P815 and the theta-posidve lymphoma, L5178Y, maintained in ascific form in DBA/2 mice, were obtained by paracentesis and washed three times in HBSS. In order to obtain allogeneically activated thymus cells (20,21), male BDF1 (C57/B16 X DBA/2) mice were lethally irradiated with 1,100 R from a Co 6° source and within 2 h were infused intravenously with 50-60 X 106 thymocytes from 5-7-wk old DBA/2 mice. On day 7 cells from recipient spleens were teased and washed in cold HBSS.…”

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confidence: 99%

RECEPTORS FOR AGGREGATED IgG ON MOUSE LYMPHOCYTES

Anderson

Grey

1974

The Journal of Experimental Medicine

187

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An autoradiographic binding assay employing 125I-labeled heat-aggregated mouse IgG2b myeloma protein (MOPC 141) was used to demonstrate receptors for IgG on 20–45% of Balb/c thymocytes and on 70–80% of splenocytes. Binding could also be shown with heat or BDB aggregates of another IgG2b (MOPC 195), with IgG1 and with human γ-globulin, but not with aggregated chicken γ-globulin, IgA, BSA, nor with aggregated Fab fragments of IgG2b. Optimum binding was obtained at 37°C. Detection of binding was dependent upon aggregate size with complexes of more than 100 IgG molecules being optimal, aggregates of 6–25 detecting splenocytes but not thymocytes, and aggregates of less than 6 binding to a negligible extent. Comparison of grain counts on various cell types showed mastocytoma cells (P815) and macrophages averaging 40–50 grains/cell/day, allogeneically activated thymocytes 20–30, splenocytes 2–3, L5178 lymphoma cells 1, and positive thymocytes 0.6 grains/cell/day. Double labeling experiments for surface Ig, θ-antigen, and agg IgG receptor on mouse spleen cells indicated that a relatively high density of receptor was present on about 80% of B cells, 30% of T cells, and 60% of SIg-, θ-, null cells.

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confidence: 99%

RECEPTORS FOR AGGREGATED IgG ON MOUSE LYMPHOCYTES

Anderson

Grey

1974

The Journal of Experimental Medicine

187

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“…Heterogeneity among the specifically sensitized lymphocytes involved in cellmediated reactions has been described in a variety of experimental situations (17)(18)(19)(20). The question arises therefore as to whether subpopulations of antigenactivated T cells, as defined by turnover, circulating life span, and tissue disposition, belong to the same cell line or to different cell lines.…”

Section: Discussionmentioning

confidence: 99%

The Mediator of Cellular Immunity

McGregor

Logie

1974

The Journal of Experimental Medicine

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Earlier reports in this series (1, 2) focused upon the cytokinetics and properties of the specifically sensitized lymphocytes which collaborate with macrophages in host resistance to Listeria monocytogenes. Evidence was obtained that the sensitized lymphocytes are generated in animals primarily immunized with the living organism. As a population, they have a rapid turnover, short effective life-span, and fail to recirculate efficiently from the blood to lymph. These and other findings imply that immunoblasts or "large lymphocytes" are the principal, although possibly not the exclusive, specific effector cells responsible for transferring resistance to L. monocytogenes, at least in the rat.Several lines of evidence indicate that specifically sensitized lymphocytes collaborate with macrophages in the expression of cellular resistance to infection (3, 4). Although the mechanism underlying their collaboration has not yet been determined, it is plausible to think that meaningful interactions occur locally in foci of infection. It may be significant therefore that after intravenous injection into rats, immunoblasts from the thoracic duct lymph of Listeriainfected donors localize in substantial numbers in inflammatory exudates induced in the peritoneal cavity (5). The results of the current investigation reaffirm and extend this observation by showing that immunoblasts move from the blood into bacteria-induced exudates in a functionally active condition and for reasons other than their immunological commitment. In addition, they indicate that the immigrant cells and small lymphocytes derived from them have protective properties. Materials and MethodsAnimals.--The subjects of this study were male and female (Lewis X DA) Fx hybrid rats. Cell donors weighed 180--240 g. The recipients fell into two groups with respect to body weight: rats in whom antimicrobial resistance was measured weighed 70--100g, whereas those given radioactively labeled ceils were approximately the same weight as the donors.

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“…On the other hand, the adoptive transfer of primary allograft responsivetiess to atiimals in which responsiveness has been suppressed by neonatal thymectomy (Sprent and Miller, 1971), or the induction of tolerance with Fj bone marrow (Gowans, McGregor and Gowan, 1963;Billingham et al, 1963) has beeti interpreted as indicating the mediation of primary allograft reactivity by the transferred TDL, These animals possess the cotisiderable advantage that previously lotig-standing skin allografts are destroyed soon after the injection of normal TDL so that the difference between injected and control animals is striking. However, other considerations render them suspect as assay animals for adoptive transfer experiments which presume to measure the anti-allograft competence of the injected cells.…”

Section: Identification Of Recirculating Small Lymphocytes As Mediatomentioning

confidence: 99%

“…However, there is no similar direct evidence that they are capable of mediating the initial or first-set ailograft response in vivo. Suggestive evidence that they may do so stems mainly from experiments which show that normal thoracic duct lymphocytes (TDL) can restore ailograft reactivity to either tolerant (Cowans, Cesner and McCregor, 1961;Billingham, Silvers and Wilson, 1963) or neonatally thymectomized (NTX) animals (Sprent and Miller, 1971). However, the role of TDL in breaking tolerance to long-standing allografts of skin in these situations cannot be unequivocally interpreted as a function of the injected cells.…”

Section: Introductionmentioning

confidence: 99%

The Adoptive Transfer of First‐set Allograft Responses by Recirculating Small Lymphocytes in the Rat

Dorsch

Roser

1974

Aust J Exp Biol Med

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Summary. While the immunological competence of recirculating, small lymphocytes in the thoracic duct lymph is well documented, there is no unequivocal evidence that these are the cells which mediate the rejection of first-set allografts in vivo. This evidence was sought by assaying the capacity of thoracic duct lymphocytes from normal donors to adoptively restore primary skin ailograft responses to recipients in which these responses had been suppressed by irradiation. It was found that 30 million thoracic duct lymphocytes were sufficient to restore rejection of allogeneic skin with a normal first-set tempo. The cell type responsible was identified as the recirculating small lymphocyte by showing that those cells which migrated from blood to lymph in heavily irradiated syngeneic rats were equally competent to restore first-set ailograft responses. The specificity of this restoration was shown using thoracic duct lymphocytes from tolerant donors which restored activity against third party allo-antigens but not against antigens of the strain to which the donor was tolerant. These results establish that recirculating small lymphocytes mediate the destruction of first-set aUografted tissue in vivo.

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Activation of Thymus Cells by Histocompatibility Antigens

Cited by 89 publications

References 5 publications

RECEPTORS FOR AGGREGATED IgG ON MOUSE LYMPHOCYTES

RECEPTORS FOR AGGREGATED IgG ON MOUSE LYMPHOCYTES

The Mediator of Cellular Immunity

The Adoptive Transfer of First‐set Allograft Responses by Recirculating Small Lymphocytes in the Rat

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