Activation of thermogenesis pathways in testis of diet-induced obesity mice

Qing, Hucheng; Hu, Juan; Fu, Huheng; Zhao, Zhikang; Nong, Weihua; Wang, Junli; Yang, Fenglian; Zhao, Sheng

doi:10.1016/j.repbio.2022.100652

Cited by 1 publication

(1 citation statement)

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“…These genes are established participants in controlling mitochondrial energy metabolism [44,50,51]. Given that many studies have posited their connection to cell thermogenesis [52], miR-196a may play a role in thermal balance regulation in BMECs during HS. The intricate underlying mechanisms require more detailed exploration.…”

Section: Discussionmentioning

confidence: 99%

miR-196a Promotes Proliferation of Mammary Epithelial Cells by Targeting CDKN1B

Chen,

Sun,

et al. 2023

Animals

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Heat stress (HS) has become one of the key challenges faced by the dairy industry due to global warming. Studies have reported that miR-196a may exert a role in the organism’s response to HS, enhancing cell proliferation and mitigating cellular stress. However, its specific role in bovine mammary epithelial cells (BMECs) remains to be elucidated. In this study, we aimed to investigate whether miR-196a could protect BMECs against proliferation arrest induced by HS and explore its potential underlying mechanism. In this research, we developed an HS model for BMECs and observed a significant suppression of cell proliferation as well as a significant decrease in miR-196a expression when BMECs were exposed to HS. Importantly, when miR-196a was overexpressed, it alleviated the inhibitory effect of HS on cell proliferation. We conducted RNA-seq and identified 105 differentially expressed genes (DEGs). Some of these DEGs were associated with pathways related to thermogenesis and proliferation. Through RT-qPCR, Western blotting, and dual-luciferase reporter assays, we identified CDKN1B as a target gene of miR-196a. In summary, our findings highlight that miR-196a may promote BMEC proliferation by inhibiting CDKN1B and suggest that the miR-196a/CDKN1B axis may be a potential pathway by which miR-196a alleviates heat-stress-induced proliferation arrest in BMECs.

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