Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain

Carballeda, Juan Manuel; Zoth, Silvina Chimeno; Gómez, Emma Laura Alfaro; Gravisaco, Marí­a José; Berinstein, Analía

doi:10.1016/j.imbio.2011.03.003

Cited by 22 publications

(23 citation statements)

References 31 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Mean values of each group were calculated and compared by Student´s t test (p< 0.05, as statistically significant). Results from Experiment 1 showed that, as previously described (Carballeda et al, 2011;Kim et al, 2000), IBDV inoculation of chickens induced T-lymphocyte infiltration in bursae at 5 dpi (days post infection), together with a decrease in Bu1+ cells (G2, Fig. 2A).…”

Section: A N U S C R I P Tsupporting

confidence: 80%

“…Oligonucleotides to amplify regions of cytokines and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, control) genes were previously described (Chimeno Zoth et al, 2012). Amplifications and detections were performed as previously described (Haghighi et al, 2008, Carballeda et al, 2011. Quantification was performed with SYBR ® Green Master Mix Kit (Applied Biosystems, Warrington, UK).…”

Section: A N U S C R I P Tmentioning

confidence: 99%

See 1 more Smart Citation

Immunomodulatory effect of baculovirus in chickens: How it modifies the immune response against infectious bursal disease virus

Zoth

Carballeda

Gravisaco³

et al. 2016

Antiviral Research

Self Cite

View full text Add to dashboard Cite

Several reports have shown that baculoviruses (BVs) have strong adjuvant properties on the mammalian immune system. Recent studies of our group demonstrated the ability of BV to stimulate the innate immunity in chickens. In this investigation, we aimed to assess the potential antiviral effect of BV given both, before and after infectious bursal disease virus (IBDV). In the first case, specific pathogen free chickens were intravenously inoculated with 5 × 10(7) pfu of Autographa californica nuclear polyhedrosis virus and 3 h later were orally administered 2.5 × 10(5) egg infectious doses 50 of IBDV. In the second case, chickens received IBDV 3 h before BV inoculation. Five days later, chickens were bled and euthanized. RNA from the bursa was analyzed for cytokine production. Also, bursae were used for virus recovery, and processed for lymphocyte isolation. The results showed that the administration of BV 3 h after the inoculation with IBDV produced important changes in the effect that IBDV causes in the bursa. BV reduced the infiltration of T lymphocytes, decreased the expression pattern of IL-6 and IFN-γ and inhibited IBDV replication. The results herein presented demonstrate that this Lepidopteran virus shows antiviral activity in chickens under experimental conditions. Investigations under field conditions have to be done to probe this strategy as a valuable sanitary tool for the treatment and prevention of chicken diseases.

show abstract

Section: A N U S C R I P Tsupporting

confidence: 80%

Section: A N U S C R I P Tmentioning

confidence: 99%

Immunomodulatory effect of baculovirus in chickens: How it modifies the immune response against infectious bursal disease virus

Zoth

Carballeda

Gravisaco³

et al. 2016

Antiviral Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Vaccination is currently the most effective way to control IBDV, and conventional vaccines are attenuated viruses based on mild or intermediate strains [37] thus avoiding vaccination side effects such as immunosuppression and clinical disease signs [1]. However, these vaccines are less effective against the diverse and vv IBDV strains that have arisen more recently.…”

Section: Discussionmentioning

confidence: 99%

Protective Oral Vaccination against Infectious bursal disease virus Using the Major Viral Antigenic Protein VP2 Produced in Pichia pastoris

et al. 2013

View full text Add to dashboard Cite

Infectious bursal disease virus (IBDV) causes economically important immunosuppressive disease in young chickens. The self-assembling capsid protein (VP2) from IBDV strain IR01 was expressed in Pichia pastoris resulting in the formation of homomeric, 23-nm infectious bursal disease subviral particles (IBD-SVPs) with a yield of 76 mg/l before and 38 mg/l after purification. Anti-IBDV antibodies were detected in chickens injected with purified IBD-SVPs or fed with either purified IBD-SVPs or inactivated P. pastoris cells containing IBD-VP2 (cell-encapsulated). Challenge studies using the heterologous classical IBDV strain (MB3) showed that intramuscular vaccination with 20 µg purified IBD-SVPs conferred full protection, achieved complete virus clearance and prevented bursal damage and atrophy, compared with only 40% protection, 0–10% virus clearance accompanied by severe atrophy and substantial bursal damage in mock-vaccinated and challenge controls. The commercial IBDV vaccine also conferred full protection and achieved complete virus clearance, albeit with partial bursal atrophy. Oral administration of 500 µg purified IBD-SVPs with and without adjuvant conferred 100% protection but achieved only 60% virus clearance with adjuvant and none without it. Moderate bursal damage was observed in both cases but the inclusion of adjuvant resulted in bursal atrophy similar to that observed with live-attenuated vaccine and parenteral administration of 20 µg purified IBD-SVPs. The oral administration of 250 mg P. pastoris cells containing IBD-VP2 resulted in 100% protection with adjuvant and 60% without, accompanied by moderate bursal damage and atrophy in both groups, whereas 25 mg P. pastoris cells containing IBD-VP2 resulted in 90–100% protection with moderate bursal lesions and severe atrophy. Finally, the oral delivery of 50 µg purified IBD-SVPs achieved 40–60% protection with severe bursal lesions and atrophy. Both oral and parenteral administration of yeast-derived IBD-VP2 can therefore induce a specific and protective immune response against IBDV without affecting the growth rate of chickens.

show abstract

“…Changes in immune cell frequency were analyzed by flow cytometry using mononuclear cells isolated from bursae and stained with different combinations of monoclonal antibodies, as described by Carballeda et al (2011). The results are summarized in Table 1.…”

Section: Recombinant Canarypox Virus Expressing the Vp2 Protein Of Inmentioning

confidence: 99%

Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens

et al. 2016

View full text Add to dashboard Cite

Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain

Cited by 22 publications

References 31 publications

Immunomodulatory effect of baculovirus in chickens: How it modifies the immune response against infectious bursal disease virus

Immunomodulatory effect of baculovirus in chickens: How it modifies the immune response against infectious bursal disease virus

Protective Oral Vaccination against Infectious bursal disease virus Using the Major Viral Antigenic Protein VP2 Produced in Pichia pastoris

Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens

Contact Info

Product

Resources

About