Activation of the early chemiluminescence response of human neutrophils by combined treatment with tumor necrosis factor (TNF-?) and calcium ionophore A23187

Shepetkin, I. E.; Borunov, E. V.; Skutina, I. L.; Naumov, Sergey

doi:10.1007/bf00842671

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1993

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“…The involvement of PKC [40. 41] and the calmodulin pathway [31] in the mechanism of secretion of C3 (Fig. 7) is also suggested, although other studies must be performed to add more fine details about the role and nature of this C3 secreted by human PMNs.…”

Section: Discussionmentioning

confidence: 99%

The Secretion of the Third Component of Complement (C3) by Human Polymorphonuclear Leucocytes from both Normal and Systemic Lupus Erythematosus Cases

Faried¹,

Tachibana²,

Okuda³

1993

Scand J Immunol

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Recently, murine peritoneal exudate polymorphonuclear leucocytes (PMNs) have been proved to secrete complement C3. In this report we show the secretion of C3 by normal human blood PMNs. ELISA assay was used to detect secreted C3 in culture supernatants of PMNs, while immunoperoxidase staining was used for intracellular C3 detection. 12-o-tetradecanoyl phorbol 13 acetate (TPA) had a flushing effect on C3 secretion by PMNs but not macrophages, suggesting a special C3 storing capability in PMNs. Dioctanoyl glycerol, mezerein and calcium ionophore A23187 caused the same marked increase in C3 secretion by PMNs. This suggests the contribution of protein kinase C and the calmodulin pathway in the mechanism of C3 secretion, similar to murine peritoneal exudate PMNs. In some cases of systemic lupus erythematosus, C3 secretion by blood PMNs was increased but no similar response to TPA could be detected.

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Section: Discussionmentioning

confidence: 99%