Activation of the cellular oncogene c-erbB by ltr insertion: Molecular basis for induction of erythroblastosis by avian leukosis virus

Fung, Y. K.; Lewis, Wynne G.; Crittenden, L. B.; Kung, Hsing Jien

doi:10.1016/0092-8674(83)90417-8

Cited by 287 publications

(158 citation statements)

References 31 publications

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“…RAV-1, a prototype ALV, was used to inoculate 1-day-old line 15, chicks. The development of erythroblastosis and collection of leukemic samples were similar to those described previously (9).…”

Section: Methodsmentioning

confidence: 49%

“…DNA samples (25 ,sg) were digested with restriction enzymes under conditions recommended by the supplier (Bethesda Research Laboratories). Digested DNAs were ethanol-precipitated, dissolved in 10 mM Tris Cl, pH 8.0/1 mM EDTA, and then electrophoresed in 0.7% agarose gel, transferred to nitrocellulose, and hybridized with the appropriate radioactive probes (9,11).…”

Section: Methodsmentioning

confidence: 99%

“…The data indicate that, upon activation, c-erbB can assume an oncogenic role similar to that of its viral counterpart. In our previous communication, we showed a strong correlation between ALV-induced structural alterations of the c-erbB gene and the development of erythroblastosis (9). No alteration of c-erbA (the cellular homolog of the other oncogene of AEV) was found in any of the samples analyzed.…”

mentioning

confidence: 99%

“…At least seven different EcoRI-digestion patterns of c-erbB were identified (ref. 9 and unpublished data). Several of the erbB-related fragments could not be accounted for by the published map of c-erbB (10).…”

mentioning

confidence: 99%

“…This activation of c-myc is accomplished by the insertion of an ALV provirus, which carries strong promoter/enhancer sequences, near the c-myc gene. We recently reported evidence suggesting that ALV induces erythroblastosis by a similar mechanism, with proviruses inserted near another host oncogene, c-erbB (9). c-erbB is the cellular homolog of one of the oncogenes carried by avian erythroblastosis virus (AEV), an acute oncogenic retrovirus known to induce rapid erythroblastosis in chickens (1,10).…”

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confidence: 99%

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c-erbB activation in avian leukosis virus-induced erythroblastosis: clustered integration sites and the arrangement of provirus in the c-erbB alleles.

Raines

Lewis

Crittenden

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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There is considerable evidence that links the activation of cellular genes to oncogenesis. We previously reported that structural rearrangements in the cellular oncogene c-erbB correlate with the development of erythroblastosis induced by avian leukosis virus (ALV). c-erbB recently has been shown to be related to the gene encoding epidermal growth factor receptor. We now have characterized the detailed mechanisms of c-erbB activation by ALV proviruses. We report here that the ALV proviral integration sites are clustered 5' to the region where homology to v-erbB starts, suggesting that interruption in this region of c-erbB is important for its activation. The proviruses are oriented in the same transcriptional direction as c-erbB and usually are full-size. The latter finding is in contrast to the frequent deletions observed within the cmyc-linked proviruses in B-cell lymphomas. We have also identified a second c-erbB allele, which differs from the previously known allele primarily by a deletion in an intron region. This allele is also oncogenic upon mutation by an ALV provirus.

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Section: Methodsmentioning

confidence: 49%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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