“…Thrice cloned subline of SK-N-SH cells 146 Expresses DAT 148,150,151 Expresses DA-β-hydroxylase and TH 504 After differentiation, presents a functionally mature neuronal phenotype 147,148 Limited synthesis of catecholamines (DA and NA) as a result of the deficiency in dihydroxyphenylalanine decarboxylase 504 Exhibits specific uptake of NA 503 Expresses proteins of NFs 503 Expresses opioid, muscarinic, and nerve growth factor (NGF) receptors 503 May differentiate upon treatment with a variety of agents [147][148][149][150][151][152][153][154][155] or substrates' deprivation 151,156,157 PC12 cell line Established from a rat pheochromocytoma 187 Expresses receptors coupled to G-proteins 191 Expresses AChE, ChAT and ACh synthase 505 May differentiate upon treatment with a variety of agents 186,189,[191][192][193][194][197][198][199] Low DA-β-hydroxylase activity 187 Expresses Na + , K + , and Ca 2+ channels 191 Ability to synthesize and store ADR and DA, and sometimes NA, which may be released upon depolarization in a Ca 2+ -dependent manner 190 Difficulty in adherence to tissue culture plates 200 C6 cell line Express Ras, Ras GTP activator protein…”