Activation of phospholipase D by osmotic cell swelling

Tomassen, Sebastian F.B.; Wijk, Thea van der; Jonge, Hugo R. de; Tilly, Ben C.

doi:10.1016/j.febslet.2004.04.063

Cited by 11 publications

(1 citation statement)

References 35 publications

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“…The activity of PLDs has been reported to be sensitive to mechanical stress, such as mechanical stretching, membrane tension surge or osmotic stress (Diz-Muñoz et al, 2016;Hornberger et al, 2006;Lin and Liu, 2019;Tomassen et al, 2004). However, how exactly PLD is activated upon mechanical stimulation remains unclear.…”

Section: Discussionmentioning

confidence: 99%

An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation

Loh

Chuang

Lin

et al. 2019

Journal of Cell Science

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Internalization of macromolecules and membrane into cells through endocytosis is critical for cellular growth, signaling and plasma membrane (PM) tension homeostasis. Although endocytosis is responsive to both biochemical and physical stimuli, how physical cues modulate endocytic pathways is less understood. Contrary to the accumulating discoveries on the effects of increased PM tension on endocytosis, less is known about how a decrease of PM tension impacts on membrane trafficking. Here, we reveal that an acute decrease of PM tension results in phosphatidic acid (PA) production, F-actin and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P 2 ]enriched dorsal membrane ruffling and subsequent macropinocytosis in myoblasts. The PA production induced by decreased PM tension depends on phospholipase D2 (PLD2) activation via PLD2 nanodomain disintegration. Furthermore, the 'decreased PM tension-PLD2-macropinocytosis' pathway is prominent in myotubes, reflecting a potential mechanism of PM tension homeostasis upon intensive muscle stretching and relaxation. Together, we identify a new mechanotransduction pathway that converts an acute decrease in PM tension into PA production and then initiates macropinocytosis via actin and PI(4,5)P 2 -mediated processes.

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Section: Discussionmentioning

confidence: 99%

An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation

Loh

Chuang

Lin

et al. 2019

Journal of Cell Science

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Autocrine signaling involved in cell volume regulation: The role of released transmitters and plasma membrane receptors

Franco

Panayiotidis

Paz

2008

Journal Cellular Physiology

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Cell volume regulation is a basic homeostatic mechanism transcendental for the normal physiology and function of cells. It is mediated principally by the activation of osmolyte transport pathways that result in net changes in solute concentration that counteract cell volume challenges in its constancy. This process has been described to be regulated by a complex assortment of intracellular signal transduction cascades. Recently, several studies have demonstrated that alterations in cell volume induce the release of a wide variety of transmitters including hormones, ATP and neurotransmitters, which have been proposed to act as extracellular signals that regulate the activation of cell volume regulatory mechanisms. In addition, changes in cell volume have also been reported to activate plasma membrane receptors (including tyrosine kinase receptors, G-protein coupled receptors and integrins) that have been demonstrated to participate in the regulatory process of cell volume. In this review, we summarize recent studies about the role of changes in cell volume in the regulation of transmitter release as well as in the activation of plasma membrane receptors and their further implications in the regulation of the signaling machinery that regulates the activation of osmolyte flux pathways. We propose that the autocrine regulation of Ca2+-dependent and tyrosine phosphorylation-dependent signaling pathways by the activation of plasma membrane receptors and swelling-induced transmitter release is necessary for the activation/regulation of osmolyte efflux pathways and cell volume recovery. Furthermore, we emphasize the importance of studying these extrinsic signals because of their significance in the understanding of the physiology of cell volume regulation and its role in cell biology in vivo, where the constraint of the extracellular space might enhance the autocrine or even paracrine signaling induced by these released transmitters.

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Effects of hyposmotic stress on exocytosis in isolated turbot, Scophthalmus maximus, hepatocytes

et al. 2006

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The effect of hyposmotic shock on exocytosis was examined in isolated hepatocytes of turbot, a marine flatfish, using the molecular probe FM1-43. Sudden exposure to a reduced osmolality caused an increase in cell exocytic activity related to the osmotic gradient between intra- and extracellular fluids. Cytoskeletal microtubules could contribute to this hyposmotic-induced exocytosis since colchicine inhibited the process. Protein kinase C, phosphatidylinositol-3 kinase, phospholipases A2, C and D could constitute key enzymes in the mechanism since their inhibition by specific agents altered the hyposmotic-induced exocytic activity. Moreover, arachidonic acid and derivates from the 5-lipoxygenase pathway as well as calcium could participate in the process. As regulatory volume decrease (RVD) exhibited by turbot hepatocytes following hyposmotic stimulation involves similar features, a potential role of exocytosis in volume regulation is suggested. In particular, exocytosis could serve RVD by contributing to ATP release since this latter process similarly appeared to be phospholipase D-dependent and related to the osmotic gradient. This study provides the first evidence of a volume-sensitive exocytosis that could aim at volume constancy in a marine teleost fish cell type.

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Activation of phospholipase D by osmotic cell swelling

Cited by 11 publications

References 35 publications

An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation

An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation

Autocrine signaling involved in cell volume regulation: The role of released transmitters and plasma membrane receptors

Effects of hyposmotic stress on exocytosis in isolated turbot, Scophthalmus maximus, hepatocytes

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