Activation of Nrf2 by Ischemic Preconditioning and Sulforaphane in Renal Ischemia/Reperfusion Injury: a Comparative Experimental Study

Shokeir, Ahmed A.; Barakat, Nashwa; Hussein, Abdelaziz M.; Awadalla, Amira; Harraz, Ahmed M.; Khater, Sherry M.; Hemmaid, Kamel Zaki; Kamal, Anand

doi:10.33549/physiolres.932834

Cited by 54 publications

(33 citation statements)

References 31 publications

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“…In consistent with our study, administration of EPO along with sodium selenite decreased oxidative stress and activated PI3K/NO signaling pathway (Liu et al 2015) in a rat ischemia-reperfusion induced renal injury model. Shokeir et al (2015) documented that combination of IPC and sulforaphone offered more improvements in the antioxidant enzymes and inflammatory state, but not the apoptotic markers.…”

Section: Discussionmentioning

confidence: 99%

Up-regulation of nitric oxide synthases by erythropoietin alone or in conjunction with ischemic preconditioning in ischemia reperfusion injury of rat kidneys

Elshiekh

Kadkhodaee²,

Seifi³

et al. 2017

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The effects of erythropoietin (EPO) alone or in conjunction with ischemic preconditioning (IPC) on nitric oxide synthase as well as comparing their effects on oxidative stress and proinflammatory cytokines are studied. Rats underwent bilateral renal ischemia of 50 min followed by 24 h reperfusion. They were administered EPO (5000 iu/kg i.p.) and/or subjected to IPC and sacrificed after 24 h, then plasma and tissue samples were obtained. Treatment of either EPO or IPC and their combination attenuates oxidative stress, decreases histological damages, inhibits proinflammatory response, and up-regulates iNOS and eNOS gene expression compared to IR group. In addition, EPO+IPC and EPO treatment produced significant up-regulation in iNOS gene expression compared to IPC group. In IPC and EPO+IPC groups, more powerful effect on up-regulation of eNOS gene expression was shown compared to EPO group. Our findings suggest that treatment with EPO or IPC and their combination improve renal function and preserve tubular damage induced by IR injury. These advantageous effects were closely related to reducing oxidative stress, suppressing proinflammatory response and enhancing generation of NO. IPC was more powerful in enhancement of eNOS gene expression compared to EPO that was more effective in increasing of iNOS gene expression.

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Section: Discussionmentioning

confidence: 99%

Up-regulation of nitric oxide synthases by erythropoietin alone or in conjunction with ischemic preconditioning in ischemia reperfusion injury of rat kidneys

Elshiekh

Kadkhodaee²,

Seifi³

et al. 2017

gpb

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“…Reactive oxygen species (ROS), apoptosis, cytokines, chemokines, and leukocytes activation play an important role in its underlying mechanisms (Jang and Rabb, 2009;Sharfuddin and Molitoris, 2011), therefore, it is imperative to find effective therapies and elucidate molecular mechanisms by which renal I/R injury may be attenuated. Antioxidants such as sulforaphane, oxymatrine and gelsemine have been demonstrated to protect murine kidneys against I/R injury (Shokeir et al, 2015;Jiang et al, 2015;Lin et al, 2015). These findings indicated that renal I/R injury might be ameliorated via targeting oxidative stress.…”

Section: Discussionmentioning

confidence: 92%

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Elsaid¹,

Khalil²,

Ibrahim³

et al. 2019

Acta Sci Pol Technol Aliment

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Background. The present work was designed to study the effects of methanolic stevia extracts and aerobic exercise and combination of both on renal I/R injury in male rats. Methods. 60 adult male Sprague-Dawley rats were subdivided into five equal groups as sham, control, exercise, stevia, and stevia plus exercise group. After 5 weeks of exercise and stevia, animals were exposed to 45 min of left renal ischemia and right nephrectomy followed by reperfusion. Serum creatinine, creatinine clearance, fractional Na excretion (FE Na +), malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT) levels in kidney tissues were measured. Also, renal histopathology and the expression of caspase-3 by immunohistochemical examination were done. Results. The results showed that stevia, exercise or combination of stevia and exercise caused a significant decrease in serum level of creatinine (p < 0.001) and FE Na + (p < 0.001) and an increase in creatinine clearance (p < 0.001). Moreover, this caused a significant decrease in (MDA; p < 0.046) and an increase in GSH (p < 0.01) and CAT (p < 0.01), as well as causing a significant decrease in caspase 3 expression compared to the control group. Conclusion. Pretreatment with either stevia or exercise of combination of both seem to have protective effects on renal I/R injury. However, the protective effect of exercise against renal I/R injury seems to be less than stevia. These effects might be due to attenuation of oxidative stress and apoptosis in kidney tissues.

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“…Selenium upregulated antioxidants in a pig model of transplanted kidneys (ischemia-reperfusion injury) 88 . Sulforaphane, a natural dietary isothiocyanate, has been shown to be renoprotective and able to improve kidney function in an animal renal IRI model 89 . In this study, Shokeir et al showed that ischemic preconditioning and sulforaphane both enhanced gene expression (Nrf2, heme oxygenase-1 HO-1, NADPH-quinone oxidoreductase1 NQO-1) and diminished the inflammatory (TNF-α, IL-1, ICAM-1) and apoptotic markers (caspase-3), with better results for sulforaphane alone.…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #1 of "Oxidative stress as a potential target in acute kidney injury (v0.1)"

2019

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Background Acute kidney injury (AKI) is a major problem for health systems being directly related to short and long-term morbidity and mortality. In the last years, the incidence of AKI has been increasing. AKI and chronic kidney disease (CKD) are closely interconnected, with a growing rate of CKD linked to repeated and severe episodes of AKI. AKI and CKD can occur also secondary to imbalanced oxidative stress reactions, inflammation and apoptosis. The kidney is particularly sensitive to oxidative stress (OS). OS is known as a crucial pathogenetic factor in cellular damage, with a direct role in initiation, development and progression of AKI. The aim of thisreview is to focus on the pathogenetic role of OS in AKI in order to gain a better understanding. We exposed the potential relationships between OS and the perturbation of renal function and we also presented the redoxdependent factors that can contribute to early kidney injury. In the last decades, promising advances have been made in understanding the pathophysiology of AKI and its consequences, but more studies are needed in order to develop new therapies that can address OS and oxidative damage in early stages of AKI.

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Activation of Nrf2 by Ischemic Preconditioning and Sulforaphane in Renal Ischemia/Reperfusion Injury: a Comparative Experimental Study

Cited by 54 publications

References 31 publications

Up-regulation of nitric oxide synthases by erythropoietin alone or in conjunction with ischemic preconditioning in ischemia reperfusion injury of rat kidneys

Up-regulation of nitric oxide synthases by erythropoietin alone or in conjunction with ischemic preconditioning in ischemia reperfusion injury of rat kidneys

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Peer Review #1 of "Oxidative stress as a potential target in acute kidney injury (v0.1)"

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