Activation of neutral trehalase by fermentable sugars and cAMP in the fission yeast Schizosaccharomyces pombe

Carrillo, Dolores

doi:10.1016/0378-1097(92)90132-8

Cited by 8 publications

(15 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…pombe results in activation of the neutral trehalase (EC 3.2.1 .28) and that this stimulation is an energy-requiring posttranslational event which can also be triggered both in repressed and derepressed growing cells by adding cAMP to the incubation buffer (Carrillo et al, 1992). In the present report, we show that the increase in trehalase activity which occurs after addition of the above inducers is indeed preceded by a rapid increase in intracellular CAMP.…”

Section: Introductionsupporting

confidence: 54%

Cyclic AMP signalling pathway and trehalase activation in the fission yeast Schizosaccharomyces pombe

1994

View full text Add to dashboard Cite

The response of derepressed cells of Schizosaccharomyces pombe to the addition of glucose included a marked and reversible activation of neutral trehalase that was not produced in repressed cells. The protein synthesis inhibitor cycloheximide, the protonophore 2,4dinitrophenol or the uncoupler sodium azide also enhanced trehalase activity in derepressed cells provided glucose was present in the incubation assays. However, only 2,4=dinitrophenol or cycloheximide was able to induce trehalase activation in repressed cells. Stimulation of trehalase by these compounds was preceded in all cases by a rapid increase in adenosine 3'-5'-cyclic monophosphate (CAMP) content. Since exogenous CAMP can activate trehalase both in repressed and derepressed growing cells, the results provide evidence for the existence of an induced CAMP signalling pathway in the fission yeast with several entries for trehalase activation. The correlation between CAMP increase and trehalase activation was not maintained when the enzyme was heat-shock-activated, supporting the concept that trehalase activity can be also enhanced in cells by another mechanism in which CAMP does not act as second messenger.

show abstract

Section: Introductionsupporting

confidence: 54%

Cyclic AMP signalling pathway and trehalase activation in the fission yeast Schizosaccharomyces pombe

1994

View full text Add to dashboard Cite

show abstract

“…This fact might reflect a higher ATP content in these cells than in derepressed cells of Schix. pombe, where trehalase activation by the same agents is energy-dependent and requires an exogenous supply of sugar (Carrillo et al, 1992).…”

Section: Trehalase and Camp In Derepressed Cellsmentioning

confidence: 99%

Activation of cytoplasmic trehalase by cyclic-AMP-dependent and cyclic-AMP-independent signalling pathways in the yeast Candida utilis

et al. 1995

View full text Add to dashboard Cite

Derepressed cells of Candida uti/is suspended in buffer exhibited both a transient CAMP-mediated signal and a marked activation of cytoplasmic trehalase when supplemented with glucose. Nitrogen sources or protein synthesis inhibitors, as well as protonophores or uncouplers, were also able to cause trehalase stimulation in derepressed cells even in the absence of the sugar. The increase in trehalase activity caused by nitrogen sources or protein synthesis inhibitors was not accompanied by changes in CAMP levels. Moreover, acridine orange inhibited both the CAMP signal and the glucoseinduced activation of trehalase without affecting the increase in trehalase activity caused by nitrogen sources or protein synthesis inhibitors. These results suggest that CAMP is not involved as second messenger in the signal for trehalase stimulation induced by the latter compounds. By contrast, the addition of glucose to repressed cells suspended in buffer failed to cause the CAMP-mediated glucose signal and sugar-induced trehalase activation. No significant changes in either trehalase activity or CAMP concentration were observed upon addition to these cells of asparagine, cycloheximide, anisomycin or other agents, including protonophores and uncouplers. However, heat treatment of repressed cultures resulted in a moderate increase in trehalase activity with negligible change in CAMP levels, whereas such an effect was not observed in derepressed cultures. The thermally induced increase in trehalase activity was dependent on de now0 protein synthesis and required the presence of glucose. Since in all cases the enzyme activated in wiwo was deactivated in vitm by phosphatase our data support the idea that in C. utilis there are at least three independent mechanisms to increase trehalase activity, involving different, but overlapping, phosphorylation pathways.

show abstract

“…The existence of a cAMP signaling pathway regulating trehalase activity has been described also in Sch. pombe and C. utilis (Carrillo et al, 1992(Carrillo et al, , 1994(Carrillo et al, , 1995. Hence, we examined possible changes in the intracellular cAMP content under the different conditions in which trehalase of Pa. tannophilus was activated.…”

Section: Trehalase and Intracellular Camp Levelsmentioning

confidence: 99%

“…By contrast, the regulatory trehalases show pH optimum close to neutrality, low thermal stability, and cytosolic location, as well as changes in enzyme activity that are dependent on the reversible phosphorylation of the enzyme protein (Ortiz et al, 1983;Uno et al, 1983;Thevelein, 1984a). Some species, including the budding yeasts Saccharomyces cerevisiae (Londesborough and Varimo, 1984;Mittenbü hler and Holzer, 1988;App and Holzer, 1989) and Candida utilis (Arguelles and Gacto, 1985;Carrillo et al, 1995), together with the fission yeast Schizosaccharomyces pombe (De Virgilio et al, 1991;Carrillo et al, 1992), possess both types of trehalase, whereas others, like Rhodotorula rubra, appear to show only one (Mansure et al, 1992). We have found that in Pa. tannophilus trehalase is the molecular target of a cAMP-dependent signaling pathway which shows both similarities and significant differences with respect to other pathways previously described in S. cerevisiae, C. utilis, and Sch.…”

mentioning

confidence: 95%

Posttranslational Regulatory Control of Trehalase Induced by Nutrients, Metabolic Inhibitors, and Physical Agents inPachysolen tannophilus

Soto

Fernández

Vicente-Soler

et al. 1996

Fungal Genetics and Biology

View full text Add to dashboard Cite

Activation of neutral trehalase by fermentable sugars and cAMP in the fission yeast Schizosaccharomyces pombe

Cited by 8 publications

References 8 publications

Cyclic AMP signalling pathway and trehalase activation in the fission yeast Schizosaccharomyces pombe

Cyclic AMP signalling pathway and trehalase activation in the fission yeast Schizosaccharomyces pombe

Activation of cytoplasmic trehalase by cyclic-AMP-dependent and cyclic-AMP-independent signalling pathways in the yeast Candida utilis

Posttranslational Regulatory Control of Trehalase Induced by Nutrients, Metabolic Inhibitors, and Physical Agents inPachysolen tannophilus

Contact Info

Product

Resources

About