Activation of muscarinic receptors in rat parotid acinar cells induces AQP5 trafficking to nuclei and apical plasma membrane

Cho, Gota; Bragiel, Aneta M.; Wang, Di; Pieczonka, Tomasz D.; Skowroński, Mariusz T.; Shono, Masayuki; Nielsén, Sören; Ishikawa, Yasuko

doi:10.1016/j.bbagen.2015.01.009

Cited by 21 publications

(21 citation statements)

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“…The results obtained in this study provide a further molecular basis for cholinergic impact in the airways and that methacholine dose‐dependently downregulates CREB phosphorylation at Ser133, probably by attenuating cAMP or PKA activity via G Q proteins, which in turn inhibits expression of AQP5 and leads to disruption of epithelial integrity. According to earlier work and the present results, AQP5 is expressed in the cell membrane, cytoplasm, and nucleus, and its intracellular trafficking is regulated by kinases and certain hormones . Parvin et al demonstrated that AQP5 trafficked from the intracellular vesicles to the apical membrane in response to stimulation of muscarinic receptors or to an increased intracellular calcium level in human salivary gland cells .…”

Section: Discussionsupporting

confidence: 79%

“…AQP5 is also involved in the pathophysiology of pulmonary edema, Ménière's disease, and other processes that involve abnormal transport of water . In rat parotid acinar cells, parasympathomimetics activate muscarinic receptors and induce AQP5 trafficking to the apical plasma membrane . It remains unknown whether parasympathomimetics have a similar impact on AQP5 regulation in human airway epithelium.…”

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confidence: 99%

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Dexamethasone attenuates methacholine‐mediated aquaporin 5 downregulation in human nasal epithelial cells via suppression of NF‐κB activation

Chang

Jian

Lin

et al. 2017

Int Forum Allergy Rhinol

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Section: Discussionsupporting

confidence: 79%

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confidence: 99%

Dexamethasone attenuates methacholine‐mediated aquaporin 5 downregulation in human nasal epithelial cells via suppression of NF‐κB activation

Chang

Jian

Lin

et al. 2017

Int Forum Allergy Rhinol

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“…Trigger-induced relocalization of AQP-containing vesicles to the plasma membrane is a well established regulatory mechanism for AQPs; the best studied example of this is relocalization of AQP2 to the apical membrane of the collecting duct in the mammalian kidney in response to arginine vasopressin (also called anti-diuretic hormone). There is also evidence for similar mechanisms for other AQPs including AQP1 ( 7 ), AQP5 ( 8 ), and AQP7 ( 9 ). A naturally occurring AQP2 mutant (R187C) has also been reported that is unable to relocalize in response to arginine vasopressin or to form tetramers ( 10 ).…”

Section: Introductionmentioning

confidence: 92%

Structural Determinants of Oligomerization of the Aquaporin-4 Channel

Kitchen

Conner

Bill

et al. 2016

Journal of Biological Chemistry

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The aquaporin (AQP) family of integral membrane protein channels mediate cellular water and solute flow. Although qualitative and quantitative differences in channel permeability, selectivity, subcellular localization, and trafficking responses have been observed for different members of the AQP family, the signature homotetrameric quaternary structure is conserved. Using a variety of biophysical techniques, we show that mutations to an intracellular loop (loop D) of human AQP4 reduce oligomerization. Non-tetrameric AQP4 mutants are unable to relocalize to the plasma membrane in response to changes in extracellular tonicity, despite equivalent constitutive surface expression levels and water permeability to wild-type AQP4. A network of AQP4 loop D hydrogen bonding interactions, identified using molecular dynamics simulations and based on a comparative mutagenic analysis of AQPs 1, 3, and 4, suggest that loop D interactions may provide a general structural framework for tetrameric assembly within the AQP family.

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“…In acinar cells, AQP5 traffics from intracellular vesicles to plasma membrane in response to acetylcholine [ 26 , 49 , 63 , 64 ]. In rat parotid glands, AQP5 also participates to the osmoregulation of acinar secretory granules [ 65 ].…”

Section: Role Of Aquaporins In Saliva Secretionmentioning

confidence: 99%

Aquaporins in Salivary Glands: From Basic Research to Clinical Applications

Delporte

Bryla

Perret

2016

IJMS

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Salivary glands are involved in saliva secretion that ensures proper oral health. Aquaporins are expressed in salivary glands and play a major role in saliva secretion. This review will provide an overview of the salivary gland morphology and physiology of saliva secretion, and focus on the expression, subcellular localization and role of aquaporins under physiological and pathophysiological conditions, as well as clinical applications involving aquaporins. This review is highlighting expression and localization of aquaporins in human, rat and mouse, the most studied species and is pointing out possible difference between major salivary glands, i.e., parotid, submandibular and sublingual glands.

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Activation of muscarinic receptors in rat parotid acinar cells induces AQP5 trafficking to nuclei and apical plasma membrane

Cited by 21 publications

References 71 publications

Dexamethasone attenuates methacholine‐mediated aquaporin 5 downregulation in human nasal epithelial cells via suppression of NF‐κB activation

Dexamethasone attenuates methacholine‐mediated aquaporin 5 downregulation in human nasal epithelial cells via suppression of NF‐κB activation

Structural Determinants of Oligomerization of the Aquaporin-4 Channel

Aquaporins in Salivary Glands: From Basic Research to Clinical Applications

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