Activation of miR-21 by STAT3 Induces Proliferation and Suppresses Apoptosis in Nasopharyngeal Carcinoma by Targeting PTEN Gene

Ou, Hesheng; Li, Yumei; Kang, Min

doi:10.1371/journal.pone.0109929

Cited by 65 publications

(60 citation statements)

References 66 publications

(62 reference statements)

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“…In the RT step, TransScript First-Strand cDNA Synthesis Supermix (Beijing Transgen Biotech Co., Ltd., Beijing, China) was used to synthesize the complementary DNA of STAT3, and the cycling conditions were as follows: 5 min at 25˚C, 60 min at 42˚C, 5 min at 85˚C, and hold at 4˚C. STAT3 mRNA qPCR was performed as described previously (19). The expression of STAT3 mRNA was normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA.…”

Section: Dual Luciferase Reporter Assaymentioning

confidence: 99%

Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia

Shi

Zhang

et al. 2016

Oncology Letters

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Abstract. Previous studies have demonstrated that microRNA-21 (miR-21) is an oncogene and is significantly upregulated in tumor tissue. However, its association with T-cell acute lymphoblastic lymphoma/leukemia (T-ALL) remains poorly understood. The aim of the present study was to investigate the effects of miR-21 on T-ALL cells by constructing Jurkat cells infected with recombinant adenovirus adv-miR-21 or adv-anti-miR-21. In addition, the target gene of miR-21 was identified by reverse transcription-quantitative polymerase chain reaction and western blotting. The results demonstrated that miR-21 expression in Jurkat cells was markedly upregulated. Furthermore, upregulating miR-21 expression in Jurkat cells promoted cell proliferation and invasion and decreased the apoptosis rate. By contrast, knockdown of miR-21 in Jurkat cells suppressed proliferation and invasion and increased the apoptosis rate. Furthermore, the results indicated that signal transducer and activator of transcription (STAT) 3 was targeted by miR-21, and that miR-21 inhibited STAT3 expression at the protein level rather than at the messenger RNA level. In conclusion, targeting the inhibition of miR-21 may be a novel therapeutic strategy for patients with T-ALL.

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Section: Dual Luciferase Reporter Assaymentioning

confidence: 99%

Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia

Shi

Zhang

et al. 2016

Oncology Letters

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“…Previous research on NPC plasma also investigated an increased expression of miRNA-21 in NPC patients compared with controls 15 . In addition, other studies conducted on tissue also confirmed an increase in the expression of miRNA-21 in NPC tissue compared with non-tumors [16][17][18] . In this study also found that there are significant differences between early stadium and later stadium.…”

Section: Discussionmentioning

confidence: 55%

“…Research on NPC tissue by Li et al 17 also showed an increase in regulation of miRNA 21 in stadium III and IV but when compared with its control. Ou et al 16 , which also examines the expression of miRNA in the tissues also suggests an increase in the expression of miRNA at an later stadium. In contrast, other studies on 217 plasma NPC patients conducted by Liu et al 13 , suggest an increase in the expression of miRNA 21 compared with controls by 5-10 fold, no specific differences between age and sex.…”

Section: Discussionmentioning

confidence: 97%

“…The existence of different regulation of microRNA still needs to be studied further. Ou et al 16 , states that STAT3 inhibition by siRNA can strongly decrease the expression of miRNA-21, otherwise activation of STAT3 increases the regulation of microRNA-21 in cells. In that study Ou et al 16 also showed that STAT3 functions on transformation and affects the activation of microRNA-21 16,20 .…”

Section: Discussionmentioning

confidence: 99%

“…Ou et al 16 , states that STAT3 inhibition by siRNA can strongly decrease the expression of miRNA-21, otherwise activation of STAT3 increases the regulation of microRNA-21 in cells. In that study Ou et al 16 also showed that STAT3 functions on transformation and affects the activation of microRNA-21 16,20 . Sun et al 18 also observed a decrease in the expression of miRNA-21 inducing retained cells in the G1/S phase thus confirming the role of miRNA-21 stimulation for cell division.…”

Section: Discussionmentioning

confidence: 99%

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Expression of Micro RNA-21 and 29c in Blood Plasma of Patients with Nasopharyngeal Carcinoma

Savitri¹,

Safri²,

Djamin³

et al. 2017

J. of Medical Sciences

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MicroRNA (miRNA) are small non-coding RNA molecules that work in post-transcription and can cause degradation and cell damage through the targeted gene. Damage to these cells influences the process of carcinogenesis. One of them is in nasopharyngeal carcinoma (NPC). Among families of miRNA that play a role in the pathogenesis of NPC that miRNA-21, miRNA-29c and which is closely associated with the progression, invasion and tumor metastasis. The purpose of this study was to determine the expression of miRNA-21 and miRNA-29c. Knowledge of these miRNA could help in the development of targeted therapy in carcinoma, as a biomarker diagnostic and clinical predictive and would be useful in therapeutic decision making. The research design was observational analytic study carried out in Wahidin Sudirohusodo Makassar, January-June, 2017. The collected blood plasma was isolated and then synthesized to cDNA and calculated quantitatively with qRT-PCR and analyzed using CFX manager 96. There were 52 samples consisting of 27 plasma NPC and 25 controls. From the plasma detected miRNA-21 and miRNA-29c. Expression of miRNA-21 increased 4.47795 fold compared with controls (p<0.05)) but its expression can not describe the progression of the tumor at a later stadium (p<0.05). In contrast, the expression of miRNA-29c decreased -119.317 fold compared with control, its regulation at a later stadium did not change significantly. Both miRNA-21 and miRNA-29c in blood plasma can use for NPC detection but a single microRNA examination is less able to provide an overview of progressivity.

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MiR‐24 enhances radiosensitivity in nasopharyngeal carcinoma by targeting SP1

et al. 2016

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Radioresistance remains a major problem in the treatment of patients suffering from nasopharyngeal carcinoma (NPC). A better understanding of the mechanisms of radioresistance may generate new strategies to improve NPC patients' responses to therapy. This study was designed to investigate the effect of microRNA on the radiosensitivity of NPC cells. A microRNA microarray indicated that miR‐24 was downregulated in NPC cell lines and tissues. Furthermore, cell proliferation was suppressed and radiosensitivity increased when miR‐24 was ectopically expressed in NPC cells. Specificity protein 1 (SP1) was additionally verified as a direct functional target of miR‐24, which was found to be involved in cell viability as well as the radiosensitivity of NPC cells. In conclusion, the results of this study suggest that the miR‐24/SP1 pathway contributed to the reduction in radioresistance in human NPC and that it may thus represent a therapeutic target.

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Activation of miR-21 by STAT3 Induces Proliferation and Suppresses Apoptosis in Nasopharyngeal Carcinoma by Targeting PTEN Gene

Cited by 65 publications

References 66 publications

Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia

Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/leukemia

Expression of Micro RNA-21 and 29c in Blood Plasma of Patients with Nasopharyngeal Carcinoma

MiR‐24 enhances radiosensitivity in nasopharyngeal carcinoma by targeting SP1

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