Activation of MAPKs by 1α,25(OH)2-Vitamin D3 and 17β-estradiol in skeletal muscle cells leads to phosphorylation of Elk-1 and CREB transcription factors

Ronda, Ana Carolina; Buitrago, Claudia; Colicheo, Andrea; Boland, Ana Russo de; Roldán, Emilio; Boland, Ricardo

doi:10.1016/j.jsbmb.2006.11.005

Cited by 40 publications

(36 citation statements)

References 31 publications

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“…Our data are consistent with previous evidence that RA strongly triggers neural differentiation (Rohwedel et al, 1999). Furthermore, our data demonstrate that RA induces the activation of CREB in EBs during their differentiation, which is consistent with recent studies showing that RA induces CREB phosphorylation and CREB-mediated transcription in PC12 cell clones that also undergo morphological differentiation upon incubation with RA (Radhakrishnan et al, 1998;Ronda et al, 2007). The activation of CREB by RA may result in increased DNA binding of active CREB to its cognate CRE binding sequence.…”

Section: Discussionsupporting

confidence: 93%

pCREB is Involved in Neural Induction of Mouse Embryonic Stem Cells by RA

Shan

Shen

et al. 2008

The Anatomical Record

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Mouse embryonic stem (ES) cells can be induced by various chemicals to differentiate into a variety of cell types in vitro. In our study, retinoic acid (RA), one of the most important inducers, used at a concentration of 5 mM, was found to induce the differentiation of ES cells into neural progenitor cells (NPCs). During embryoid body (EB) differentiation, the level of active cyclic AMP response element-binding protein (CREB) was relatively high when 5 mM RA treatment was performed. Inhibition of CREB activity committed EBs to becoming other germ layers, whereas increased expression of CREB enhanced NPC differentiation. Moreover, RA increased the expression of active CREB by enhancing the activity of JNK. Our research suggests that CREB plays a role in RA-induced NPC differentiation by increasing the expression of active JNK. Anat Rec,

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Section: Discussionsupporting

confidence: 93%

pCREB is Involved in Neural Induction of Mouse Embryonic Stem Cells by RA

Shan

Shen

et al. 2008

The Anatomical Record

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“…of proteins in cultured C2C12 myoblasts, thereby influencing the early expression of genes involved in cellular proliferation and differentiation (Ronda et al 2007). It was observed that for C2C12 cells (grown in DMEM with 10% FBS) incubation with 100 nM 1,25D stimulated differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin (Garcia et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Actions of 1,25(OH)2-vitamin D3 on the cellular cycle depend on VDR and p38 MAPK in skeletal muscle cells

Irazoqui¹,

Boland²,

Buitrago³

2014

Journal of Molecular Endocrinology

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Previously, we have reported that 1,25(OH) 2 -vitamin D 3 (1,25D) activates p38 MAPK (p38) in a vitamin D receptor (VDR)-dependent manner in proliferative C2C12 myoblast cells. It was also demonstrated that 1,25D promotes muscle cell proliferation and differentiation. However, we did not study these hormone actions in depth. In this study we have investigated whether the VDR and p38 participate in the signaling mechanism triggered by 1,25D. In C2C12 cells, the VDR was knocked down by a shRNA, and p38 was specifically inhibited using SB-203580. Results from cell cycle studies indicated that hormone stimulation prompts a peak of S-phase followed by an arrest in the G0/G1-phase, events which were dependent on VDR and p38. Moreover, 1,25D increases the expression of cyclin D3 and the cyclin-dependent kinase inhibitors, p21Waf1/Cip1 and p27 Kip1 , while cyclin D1 protein levels did not change during G0/G1 arrest. In all these events, p38 and VDR were required. At the same time, a 1,25D-dependent acute increase in myogenin expression was observed, indicating that the G0/G1 arrest of cells is a pro-differentiative event. Immunocytochemical assays revealed co-localization of VDR and cyclin D3, promoted by 1,25D in a p38-dependent manner. When cyclin D3 expression was silenced, VDR and myogenin levels were downregulated, indicating that cyclin D3 was required for 1,25D-induced VDR expression and the concomitant entrance into the differentiation process. In conclusion, the VDR and p38 are involved in control of the cellular cycle by 1,25D in skeletal muscle cells, providing key information on the mechanisms underlying hormone regulation of myogenesis.

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“…Other demonstrated actions of 1,25(OH) 2 D in skeletal muscle include upregulation of calcium transport [24] Baseline force % Train number COPD patients Control subjects ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲ ▲▲ ▲ ▲▲▲▲ ▲ ▲ ▲▲ ▲▲▲ ▲▲ ▲ ▲▲ ▲▲ ▲ ▲▲ ▲ ▲ ▲▲ 56 52 48 44 40 36 32 28 24 20 16 12 8 and regulation of phospholipid metabolism [25]. Nongenomic or rapid effects of vitamin D have also been documented, including regulation of intracellular calcium [26] and activation of mitogen-activated protein kinase C pathways [27].…”

Section: Discussionmentioning

confidence: 99%

Vitamin D and skeletal muscle strength and endurance in COPD

et al. 2012

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It is not known whether vitamin D levels make a significant contribution to muscle dysfunction in chronic obstructive pulmonary disease (COPD).In 104 COPD patients (mean¡SD forced expiratory volume in 1 s 44¡22 % predicted) and 100 age-and sex-matched controls, serum 25-hydroxyvitamin D (25(OH)D), 1,25-dihydroxyvitamin D (1,25(OH) 2 D) and parathyroid hormone (PTH) levels were measured and related to quadriceps strength and endurance. In a subset of 26 patients and 13 controls, quadriceps biopsy was performed and mRNA expression of myogenic regulatory factors (mrf) and fibre-specific myosin heavy chains (MHC) was determined.COPD patients were weaker and less physically active than controls. The aim of the current study was to establish whether low vitamin D levels were associated with impaired skeletal muscle function in COPD patients. A significant association in COPD patients

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Activation of MAPKs by 1α,25(OH)2-Vitamin D3 and 17β-estradiol in skeletal muscle cells leads to phosphorylation of Elk-1 and CREB transcription factors

Cited by 40 publications

References 31 publications

pCREB is Involved in Neural Induction of Mouse Embryonic Stem Cells by RA

pCREB is Involved in Neural Induction of Mouse Embryonic Stem Cells by RA

Actions of 1,25(OH)2-vitamin D3 on the cellular cycle depend on VDR and p38 MAPK in skeletal muscle cells

Vitamin D and skeletal muscle strength and endurance in COPD

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