Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection

Hu, Yongjian; Song, Gui-yuan; Zhang, Fan; Zhang, Nan; Wang, Fei; Wang, Jinglong; Wang, Xia; Wang, Taoyang; Li, Yufeng; Yan, Yi-di; Dou, Wen-tao; Cheng, Chen-yi; Xu, Ping

doi:10.1016/j.isci.2023.105932

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…But lncRNA also functions in normal tissue response to radiation injury and may be integral as markers of damage. For example, lncRNA Neat1, which has a radioprotective role, was shown to be downregulated after radiation in the liver and lungs in a mouse model [Hu et al, 2023]. Similarly, our laboratory has identified two lncRNAs, damage-induced noncoding and tumor protein p53 pathway corepressor 1 (Trp53cor1), that were significantly upregulated in whole-body irradiated mice [Aryankalayil et al, 2018a].…”

Section: Rna and Metabolites As Biomarkers For Radiation Injurymentioning

confidence: 92%

Biomarkers for Biodosimetry and Their Role in Predicting Radiation Injury

Aryankalayil¹,

Bylicky²,

Chopra³

et al. 2023

Cytogenet Genome Res

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Radiation-related normal tissue injury sustained during cancer radiotherapy or in a radiological or mass casualty nuclear incident is a major health concern. Reducing the risk and mitigating consequences of radiation injury could have a broad impact on cancer patients and citizens. Efforts to discover biomarkers that can determine radiation dose, predict tissue damage, and aid medical triage are underway. Exposure to ionizing radiation causes changes in gene, protein, and metabolite expression that needs to be understood to provide a holistic picture for treating acute and chronic radiation-induced toxicities. We present evidence that both RNA (mRNA, microRNA, long noncoding RNA) and metabolomic assays may provide useful biomarkers of radiation injury. RNA markers may provide information on early pathway alterations after radiation injury that can predict damage and implicate downstream targets for mitigation. In contrast, metabolomics is impacted by changes in epigenetics, genetics, and proteomics and can be considered a downstream marker that incorporates all these changes to provide an assessment of what is currently happening within an organ. We highlight research from the past 10 years to understand how biomarkers may be used to improve personalized medicine in cancer therapy and medical decision-making in mass casualty scenarios.

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Section: Rna and Metabolites As Biomarkers For Radiation Injurymentioning

confidence: 92%

Biomarkers for Biodosimetry and Their Role in Predicting Radiation Injury

Aryankalayil¹,

Bylicky²,

Chopra³

et al. 2023

Cytogenet Genome Res

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“…Thymol [198] miR-29a-3p [199] Ginsenoside Rg1 [17] miR-126-3p [200] Troxerutin [201] miR-146a-5p [202], miR-147a [203] miR-21-5p [181,182] ↓…”

Section: Natural-product-regulated Mirnasmentioning

confidence: 99%

“…Troxerutin attenuated myocardial ischemia and reperfusion injury-triggered apoptosis by downregulating miR-146a-5p [202]. Troxerutin exhibited radioprotection for normal epithelial cells by downregulating miR-147a, which is upregulated by radiation [203]. Vitamin C (3 g/kg) increased the survival of mice irradiated with 8 Gy [204].…”

Section: Function Of Radioprotective Natural Products In Non-cancer T...mentioning

confidence: 99%

Connection between Radiation-Regulating Functions of Natural Products and miRNAs Targeting Radiomodulation and Exosome Biogenesis

Tang

Chuang

Shiau

et al. 2023

IJMS

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Exosomes are cell-derived membranous structures primarily involved in the delivery of the payload to the recipient cells, and they play central roles in carcinogenesis and metastasis. Radiotherapy is a common cancer treatment that occasionally generates exosomal miRNA-associated modulation to regulate the therapeutic anticancer function and side effects. Combining radiotherapy and natural products may modulate the radioprotective and radiosensitizing responses of non-cancer and cancer cells, but there is a knowledge gap regarding the connection of this combined treatment with exosomal miRNAs and their downstream targets for radiation and exosome biogenesis. This review focuses on radioprotective natural products in terms of their impacts on exosomal miRNAs to target radiation-modulating and exosome biogenesis (secretion and assembly) genes. Several natural products have individually demonstrated radioprotective and miRNA-modulating effects. However, the impact of natural-product-modulated miRNAs on radiation response and exosome biogenesis remains unclear. In this review, by searching through PubMed/Google Scholar, available reports on potential functions that show radioprotection for non-cancer tissues and radiosensitization for cancer among these natural-product-modulated miRNAs were assessed. Next, by accessing the miRNA database (miRDB), the predicted targets of the radiation- and exosome biogenesis-modulating genes from the Gene Ontology database (MGI) were retrieved bioinformatically based on these miRNAs. Moreover, the target-centric analysis showed that several natural products share the same miRNAs and targets to regulate radiation response and exosome biogenesis. As a result, the miRNA–radiomodulation (radioprotection and radiosensitization)–exosome biogenesis axis in regard to natural-product-mediated radiotherapeutic effects is well organized. This review focuses on natural products and their regulating effects on miRNAs to assess the potential impacts of radiomodulation and exosome biogenesis for both the radiosensitization of cancer cells and the radioprotection of non-cancer cells.

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“…lncRNA is a class of non-protein-coding RNAs with a length of 200 nucleotides that play key roles in biological processes, such as chromatin progression, transcription, and posttranscriptional modification (16). In terms of mechanism research, lncRNAs often serve as sponges for microRNAs (miRNAs) (17)(18)(19). The latest data shows the regulatory mechanism of m6A-modified lncRNAs in cancer (20), which also indicates that m6A-modified lncRNAs might serve as potential biomarkers and therapeutic targets for human cancer.…”

Section: Introductionmentioning

confidence: 99%

Preliminary study based on methylation and transcriptome gene sequencing of lncRNAs and immune infiltration in hypopharyngeal carcinoma

Chang

et al. 2023

Front. Oncol.

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BackgroundHypopharyngeal squamous cell cancer (HSCC) is one of the most malignant tumors of the head and neck. It is not easy to detect in the early stage due to its hidden location; thus, lymph node metastasis is highly likely at diagnosis, leading to a poor prognosis. It is believed that epigenetic modification is related to cancer invasion and metastasis. However, the role of m6A-related lncRNA in the tumor microenvironment (TME) of HSCC remains unclear.MethodsThe whole transcriptome and methylation sequencing of 5 pairs of HSCC tissues and adjacent tissues were performed to identify the methylation and transcriptome profiles of lncRNAs. The biological significance of lncRNAs differentially expressing the m6A peak was analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. By constructing an m6A lncRNA-microRNA network, the mechanism of m6A lncRNAs in HSCC was analyzed. The relative expression levels of selected lncRNAs were examined by quantitative polymerase chain reaction. The CIBERSORT algorithm was used to evaluate the relative proportion of immune cell infiltration in HSCC and paracancerous tissues.ResultsBased on an in-depth analysis of the sequencing results, 14413 differentially expressed lncRNAs were revealed, including 7329 up-regulated and 7084 down-regulated lncRNAs. Additionally, 4542 up-methylated and 2253 down-methylated lncRNAs were detected. We demonstrated methylation patterns and gene expression profiles of lncRNAs of HSCC transcriptome. In the intersection analysis of lncRNAs and methylated lncRNAs, 51 lncRNAs with up-regulated transcriptome and methylation and 40 lncRNAs with down-regulated transcriptome and methylation were screened, and significantly differentiated lncRNAs were further studied. In the immune cell infiltration analysis, B cell memory was significantly elevated in cancer tissue, while γδT cell amount was significantly decreased.Conclusionm6A modification of lncRNAs might be involved in HSCC pathogenesis. Infiltration of immune cells in HSCC might provide a new direction for its treatment. This study provides new insights for exploring the possible HSCC pathogenesis and searching for new potential therapeutic targets.

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Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection

Cited by 5 publications

References 41 publications

Biomarkers for Biodosimetry and Their Role in Predicting Radiation Injury

Biomarkers for Biodosimetry and Their Role in Predicting Radiation Injury

Connection between Radiation-Regulating Functions of Natural Products and miRNAs Targeting Radiomodulation and Exosome Biogenesis

Preliminary study based on methylation and transcriptome gene sequencing of lncRNAs and immune infiltration in hypopharyngeal carcinoma

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