Activation of Leukocytes During Prolonged Physical Exercise

Kokot, K; Schaefer, Roland M.; Teschner, Markus; Gilge, U; Plass, R; Heidland, A.

doi:10.1007/978-1-4613-1057-0_6

Cited by 44 publications

(21 citation statements)

References 11 publications

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“…quadriceps muscle white portion, quadriceps muscle red portion, soleus muscle; 0 (sedentary control), 5, 15, and 25 are quantity of repetitive swimming bouts during exercise. Means § SEM; n = 6-8; *P < 0.05, **P < 0.01, ***P < 0.001 versus "sedentary control"; ▼ P < 0.05, ▼▼ P < 0.01, ▼▼▼ P < 0.001 versus "quadriceps muscle white portion"; t-test MPO is a common marker of exercise-induced degranulation of neutrophils (Kokot et al 1988;Camus et al 1998;Walsh et al 2000;Morozov et al 2003). MPO eZux depends on exercise intensity: blood MPO concentration was higher after high-intensity (85% of maximal oxygen consumption) than after moderate intensity exercise (60% of maximal oxygen consumption) ).…”

Section: Discussionmentioning

confidence: 99%

“…In particular, it was shown that intense physical exercise can induce the Portions of this paper were presented by V. Morozov in 2003 at the 6th ISEI Symposium on Exercise Muscle Metabolism and Immune Function, Copenhagen. degranulation of neutrophils that leads to an increase in plasma concentration of marker neutrophil proteins including myeloperoxidase (MPO), lactoferrin, and elastase (Kokot et al 1988;Camus et al 1998;Gleeson et al 1998;Walsh et al 2000;Morozov et al 2003;Inoue et al 2004;Morozov and Kalinski 2006). The extent of degranulation tends to increase with increasing intensity of exercise .…”

Section: Introductionmentioning

confidence: 99%

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The effects of high-intensity exercise on skeletal muscle neutrophil myeloperoxidase in untrained and trained rats

et al. 2006

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The primary purpose of this study was to examine the effects of high-intensity acute exercise on neutrophil infiltration in different muscle fiber types of untrained rats and to compare postexercise neutrophil accumulation in muscles of untrained and trained animals. The effect of high-intensity acute exercise on blood neutrophil degranulation reaction in trained animals was also elucidated. Neutrophil enzyme myeloperoxidase (MPO) was determined as a measure of neutrophil migration into muscles and blood neutrophil degranulation. Male albino rats were subjected to acute exercise and 5 weeks of training. The used model of intensive acute exercise consisted of 5, 15, and 25 intermittent swimming bouts with the addition of weight (8% of total body mass) for 1-min each, followed by 1.5-min rest intervals. MPO was analyzed in quadriceps muscle (white and red portion) and in soleus muscle 24 h after acute exercise. MPO content in resting blood plasma and neutrophils was determined 48-h following the completion of a training process. In addition, MPO content in the trained rats was measured immediately (in blood plasma and neutrophils) after and 24 h (in muscles) following a single-bout of exercise to exhaustion. The remaining two-third of the trained animals were exposed to a single-bout of nonstop swimming with the addition of 6% body mass until exhaustion. These animals were sacrificed immediately and 24 h after loaded swimming to analyze leukocyte count, MPO content in blood plasma and neutrophils and in muscles, respectively. About 24 h after exercise MPO concentrations in the red portion of quadriceps muscle and in soleus muscle were 4-7-fold higher as compared to the white portion of m. quadriceps. There was an association between the quantity of repetitive bouts of swimming and MPO content in the muscles. The duration of swimming to exhaustion of trained rats was 3.8-fold longer than untrained sedentary control. At rest, plasma MPO concentration was found to be 40% higher in trained rats compared to untrained controls (P < 0.05). Postexercise plasma MPO concentrations were significantly higher both in untrained (+137%; P < 0.05) and trained (+81%; P < 0.05) rats compared to resting values. At rest neutrophil MPO concentration was found to be 33% lower in trained rats compared to untrained controls (P < 0.05). There were no significant differences in muscle MPO concentrations between untrained and trained rats at rest. A single-bout of exercise to exhaustion produced a greater increase in MPO content in untrained compared to trained rats. The data suggest that postexercise neutrophil infiltration is more intensive in red fibers types compared to white fiber types. A smaller neutrophil infiltration in muscles of trained animals after exhaustive exercise suggests a protective effect of previous training to muscle injury.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effects of high-intensity exercise on skeletal muscle neutrophil myeloperoxidase in untrained and trained rats

et al. 2006

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“…Physical activity induces neutrophil priming for oxidative burst and activates acute phase protein release [7,8]. An increase in MPO and lysozyme plasma concentrations has been evidenced as a result of exercise [8,9].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant supplementation influences the neutrophil tocopherol associated protein expression, but not the inflammatory response to exercise

et al. 2007

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Abstract:Intense exercise induces inflammatory-like changes and oxidative stress in immune cells. Our aim was to study the effects of antioxidant diet supplementation on the neutrophil inflammatory response and on the tocopherol associated protein (TAP) expression after exhaustive exercise. Fourteen male-trained amateur runners were randomly divided in two placebo and supplemented groups. Vitamins C (152 mg/d) and E (50 mg/d) supplementation were administrated to the athletes for a month, using an almond based isotonic and energetic beverage. Non-enriched beverage was given to the placebo group. After one month, the subjects participated in a half-marathon race (21 km-run). Neutrophil TAP mRNA expression and markers of the inflammatory response were determined before, immediately after, and 3 h after finishing the half-marathon race. TAP expression increased after exercise mainly in the neutrophils of the placebo group. Exercise induced an inflammatory response in both placebo and supplemented groups, manifested with neutrophilia, increased creatine kinase and lactate dehydrogenase serum activities, neutrophil luminol chemiluminescence and myeloperoxidase release. Plasma malondialdehyde only increased in the placebo group after exercise. Diet supplementation with moderate levels of antioxidant vitamins avoids plasma damage in response to exhaustive exercise without the effects on the inflammatory process. Neutrophil degranulation and increased tocopherol associated protein could contribute to the neutrophil protection from the oxidative stress.

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“…As described in detail in previous studies (36,37), neutrophils were isolated from the peripheral blood of an adult healthy male using Mono -Poly Resolving Medium (Dainippon Pharmaceutical, Japan). Neutrophil suspensions were prepared by adjusting neutrophil counts to 3.0 × 10 3 cells/μL through dilution with HBSS (36, 37).…”

Section: Methodsmentioning

confidence: 99%

Are individuals with lower neutrophil oxidative burst activity more prone to Helicobacter pylori infection?

et al. 2008

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Helicobacter pylori infection has been reported to cause enhanced reactive oxygen species in the gastric mucosa. We examined the relationship between H. pylori infection and neutrophil function of peripheral blood. The subjects were 904 volunteers who participated in the Iwaki Health Promotion Project in 2005. 158 subjects who were infected with H. pylori in 2005 also participated in this project in 2006 and were categorized into two groups: the eradication group, in which H. pylori was successfully eradicated during the 12 month period, and the non-eradication group, in which eradication was unsuccessful or the subjects did not receive eradication therapy. The laboratory assays performed were: a titre of H. pylori antibody; neutrophil counts; and oxidative burst activity (OBA) of neutrophils. Logistic regression analysis was executed, with H. pylori infection as the dependent variable and other items as the independent variables. OBA showed an inverse association with H. pylori infection in 2005. Additionally, when comparing the eradication and non-eradication groups, the change rates of OBA between 2005 and 2006 did not show any significant difference. It was concluded that H. pylori infection does not lower OBA, but those individuals in whom OBA was lower were more prone to H. pylori infection.

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Activation of Leukocytes During Prolonged Physical Exercise

Cited by 44 publications

References 11 publications

The effects of high-intensity exercise on skeletal muscle neutrophil myeloperoxidase in untrained and trained rats

The effects of high-intensity exercise on skeletal muscle neutrophil myeloperoxidase in untrained and trained rats

Antioxidant supplementation influences the neutrophil tocopherol associated protein expression, but not the inflammatory response to exercise

Are individuals with lower neutrophil oxidative burst activity more prone to Helicobacter pylori infection?

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