“…Cell staining was performed as previously described [16]. Briefly, cytospin preparations of myeloid cells were fixed in 1.5% formaldehyde for 30 min, permeabilized in 0.5% Triton-X 100 for 20 min, and blocked in 40% FBS/40% goat serum for 30 min before incubation with antibodies directed against the viral proteins IE1/IE2 (MAb810, 1:600, or AF488 MAB810X, 1:200, Millipore, Temecula, CA), UL32 (pp150, 1:400, a kind gift from Bill Britt, University of Alabama, Birmingham), UL84 (1:500, Virusys, Taneytown, MD), UL44 (1:200, Virusys, Taneytown, MD), or UL57 (1:100, Virusys, Taneytown, MD) for one hour, followed by secondary antibodies conjugated to Alexa-Fluor 488 or Alexa-Fluor 594 (1:200, Invitrogen, Carlsbad, CA, and Jackson Immunoresearch, West Grove, PA) for another hour.…”