Activation of Kv7 channels normalizes hyperactivity of the VTA-NAcLat circuit and attenuates methamphetamine-induced conditioned place preference and sensitization in mice

Liu, E; Pang, Kunkun; Liu, Min; Tan, Xu; Hang, Zhaofang; Mu, Shouhong; Han, Weikai; Yue, Qingwei; Comai, Stefano; Sun, Jinhao

doi:10.1038/s41380-023-02218-5

Cited by 5 publications

(4 citation statements)

References 79 publications

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“…The GO enrichment analysis, respectively, revealed the top 5 terms in cellular components, biological processes, and molecular functions, and identified 19 DEmRNAs associated with these terms (Figure 5E and Table S4). Previous studies have reported the significant involvement of voltage‐gated potassium channels in mediating the rewarding effects of substance abuse 32,33 . KCNJ5 gene encodes the protein of G protein‐activated inward rectifier potassium channel 4 (GIRK4).…”

Section: Resultsmentioning

confidence: 99%

Identification of the circRNA–miRNA–mRNA network for treating methamphetamine‐induced relapse and behavioral sensitization with cannabidiol

Liu,

Wang,

Wang

et al. 2024

CNS Neurosci Ther

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AimsThis study aims to investigate the pharmacological effects and the underlying mechanism of cannabidiol (CBD) on methamphetamine (METH)‐induced relapse and behavioral sensitization in male mice.MethodsThe conditioned place preference (CPP) test with a biased paradigm and open‐field test were used to assess the effects of CBD on METH‐induced relapse and behavioral sensitization in male mice. RNA sequencing and bioinformatics analysis was employed to identify differential expressed (DE) circRNAs, miRNAs, and mRNAs in the nucleus accumbens (NAc) of mice, and the interaction among them was predicted using competing endogenous RNAs (ceRNAs) network analysis.ResultsChronic administration of CBD (40 mg/kg) during the METH withdrawal phase alleviated METH (2 mg/kg)‐induced CPP reinstatement and behavioral sensitization in mice, as well as mood and cognitive impairments following behavioral sensitization. Furthermore, 42 DEcircRNAs, 11 DEmiRNAs, and 40 DEmRNAs were identified in the NAc of mice. The circMeis2‐miR‐183‐5p‐Kcnj5 network in the NAc of mice is involved in the effects of CBD on METH‐induced CPP reinstatement and behavioral sensitization.ConclusionsThis study constructed the ceRNAs network for the first time, revealing the potential mechanism of CBD in treating METH‐induced CPP reinstatement and behavioral sensitization, thus advancing the application of CBD in METH use disorders.

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Section: Resultsmentioning

confidence: 99%

Identification of the circRNA–miRNA–mRNA network for treating methamphetamine‐induced relapse and behavioral sensitization with cannabidiol

Liu,

Wang,

Wang

et al. 2024

CNS Neurosci Ther

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“…Sensitization to methamphetamine was induced in mice using a protocol consisting of daily administration for 5 consecutive days, as previously reported [ 32 , 33 , 34 ]. Mice were injected intraperitoneally (i.p.)…”

Section: Methodsmentioning

confidence: 99%

Different Doses of Methamphetamine Are Needed to Produce Locomotor or Blood Pressure Sensitization in Mice

Busceti,

Bucci,

De Lucia

et al. 2024

Life

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Methamphetamine (METH) exposure increases locomotor sensitization. However, no study has explored the occurrence of cardiovascular sensitization. The present study, carried out in mice, analyzed the following: (i) METH sensitization extending to systolic blood pressure (SBP); (ii) a potential correlation between ambulatory and cardiovascular sensitization; and (iii) morphological alterations within meso-striatal, meso-limbic and pontine catecholamine systems including c-fos expression. Locomotor activity, SBP and occurrence of morphological alterations of catecholaminergic neurons were assessed in C57Bl/6J mice following daily i.p. injections of either saline or METH (1, 2 or 5 mg/kg) for 5 consecutive days and following 6 days of withdrawal. Reiterated exposure to the lower doses of METH (1 mg/kg and 2 mg/kg) produced in mice locomotor sensitization without altering SBP. In contrast, repeated treatment with the highest dose of METH (5 mg/kg) produced sensitization of SBP in the absence of locomotor sensitization. No morphological alterations but increases in c-fos expression within neurons of locus coeruleus and nucleus accumbens were detected. The present data suggest that METH produces plastic changes that extend beyond the motor systems to alter autonomic regulation. This cardiovascular sensitization occurs independently of locomotor sensitization. The persistency of increased blood pressure may underlie specific mechanisms operating in producing hypertension.

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“…This method was useful in the isolation of DA subtype cells that are influenced by METH consumption. DA neurons of the VTA-NAcLat circuit were identified and its gene expression was defined [82,83].…”

Section: Identifying Transcriptome Mechanismmentioning

confidence: 99%

Breaking the Chains: Advances in Substance Addiction Research through Single-Cell Sequencing, Epigenetics, and Epitranscriptomic

Filošević Vujnović,

Stanković Matić,

Saftić Martinović

et al. 2024

Future Pharmacology

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Addiction is a complex brain disease influenced by genetic, environmental, and neurological factors. Psychostimulants, cocaine, and methamphetamine influence different cell types in different brain regions, with a focus on the neurons responsible for rewarding effects in the nucleus accumbens (NAc) and ventral tegmental area (VTA). Known markers for psychostimulant-induced neuronal plasticity in combination with droplet-based high-throughput single-cell sequencing divided the heterogeneity of cell populations in NAc and VTA into clusters, where all cells of the same type do not respond equally to exposure to psychostimulants. To explain psychostimulant-induced neuronal plasticity as changes in the amplitude and phase shifts of gene expression, we focused on epigenetic mechanisms of DNA and chromatin modifications, as well as DNA accessibility. We also comment on epitranscriptomics as a novel approach in the study of messenger RNA posttranslational modification, which regulates translation and potentially localized transcription in synapses in order to address the molecular chains that connect addiction from changes in gene expression to synaptic and, finally, neuronal plasticity.

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Activation of Kv7 channels normalizes hyperactivity of the VTA-NAcLat circuit and attenuates methamphetamine-induced conditioned place preference and sensitization in mice

Cited by 5 publications

References 79 publications

Identification of the circRNA–miRNA–mRNA network for treating methamphetamine‐induced relapse and behavioral sensitization with cannabidiol

Identification of the circRNA–miRNA–mRNA network for treating methamphetamine‐induced relapse and behavioral sensitization with cannabidiol

Different Doses of Methamphetamine Are Needed to Produce Locomotor or Blood Pressure Sensitization in Mice

Breaking the Chains: Advances in Substance Addiction Research through Single-Cell Sequencing, Epigenetics, and Epitranscriptomic

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