Activation of human monocytes by Streptococcus mutans serotype f polysaccharide: immunoglobulin G Fc receptor expression and tumor necrosis factor and interleukin-1 production

Benabdelmoumene, S; Dumont, Serge; Petit, Christine; Poindron, P.; Wachsmann, Dominique; Klein, Jan Philipp

doi:10.1128/iai.59.9.3261-3266.1991

Cited by 34 publications

(26 citation statements)

References 22 publications

(19 reference statements)

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“…2). These results are similar to other reports showing that S. mutans stimulates the production of TNF-a and IL-1b in human peripheral blood mononuclear cells or human monocytes, or the S. mutans OMZ175 protein I/II induces the production of TNF-a and IL-1b in human synoviocytes (Benabdelmoumene et al, 1991;Soell et al, 1994;Jiang et al, 1999;Gourieux et al, 2001). Nuclear factor-jB is a multiunit transcription factor that plays a key role in the induction of genes for proinflammatory cytokines (Collart et al, 1990;Ziegler-Heitbrock et al, 1993;Neff et al, 2001) as well as many other immunoregulatory genes (Baeuerle, 1991).…”

Section: Discussionsupporting

confidence: 92%

Tumor necrosis factor‐α and interleukin‐1β expression pathway induced by Streptococcus mutans in macrophage cell line RAW 264.7

Kim

et al. 2012

Molecular Oral Microbiology

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Streptococcus mutans, a major etiological agent of dental caries, frequently causes systemic disease, such as subacute bacterial endocarditis, if it enters the bloodstream. In this study, the production pathways of the proinflammatory cytokines, tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), induced by S. mutans in mouse macrophage were examined using a quantitative real-time polymerase chain reaction and an enzyme-linked immunosorbent assay. The S. mutans stimulated the expression of TNF-α and IL-1β mRNA at a multiplicity of infection of 1 : 100, which increased at 2 and 4 h, respectively, to 24 h. It also induced the production of high levels of the TNF-α and IL-1β proteins, which increased at 2 h and reached a peak at 4 and 24 h, respectively. Nuclear factor-κB (NF-κB) was activated and reached a maximum level 30 min after the S. mutans treatment. The expression of TNF-α and IL-1β mRNA and protein was suppressed by the treatment with pyrrolidine dithiocarbamate, an NF-κB inhibitor. The S. mutans-induced TNF-α expression was suppressed by the presence of SB203580, a p38 mitogen-activated protein (MAP) kinase inhibitor, or SP600125, a Jun N-terminal kinase (JNK) MAP kinase inhibitor. On the other hand, IL-1β expression was inhibited by extracellular signal-regulated kinase (ERK)/p38/JNK MAP kinase inhibitor pretreatment. In addition, TNF-α production was suppressed more in the Toll-like receptor 2(-/-) (TLR2(-/-)) macrophages than in the TLR4(-/-) macrophages, whereas IL-1β production was suppressed more in the TLR4(-/-) macrophages than in the TLR2(-/-) macrophages. These results show that S. mutans stimulates the production of TNF-α and IL-1β in the mouse macrophage cell line, RAW 264.7, by activating ERK/p38/JNK, and NF-κB through TLR2 and TLR4, respectively.

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Section: Discussionsupporting

confidence: 92%

Tumor necrosis factor‐α and interleukin‐1β expression pathway induced by Streptococcus mutans in macrophage cell line RAW 264.7

Kim

et al. 2012

Molecular Oral Microbiology

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“…Antisera production. Polyclonal anti-SR, anti-rhTNF-cx, and anti-rhIL-1 3 sera were raised in rabbits by several injections of 50 tLg of either SR or TNF-ot or IL-1B suspended in Freund's incomplete adjuvant (5). The IgG fractions of anti-SR, anti-rhTNF-ot, and anti-rhIL-13 sera were purified by chromatography on DEAE Trisacryl M essentially by the method of Corthier et al (8).…”

Section: Materuils and Methodsmentioning

confidence: 99%

“…It has been postulated that acute inflammation and subsequent tissue damage could result from the deposition of either streptococcal antigens or antigen-antibody complexes within the tissues (37) or from the stimulation of the host immune response by certain antigens, leading to the synthesis of either antibodies that cross-react with host tissues (20) or cytokines, which are believed to play an important role in these chronic inflammatory diseases. We recently showed that serotype polysaccharide from Streptococcus mutans OMZ 175 (serotype f), a member of the viridans streptococci, cah stimulate human monocytes to release tumor necrosis factor alpha (TNF-ot) and interleukin 1 (IL-1,B) (5).…”

mentioning

confidence: 99%

Binding of Streptococcus mutans SR protein to human monocytes: production of tumor necrosis factor, interleukin 1, and interleukin 6

et al. 1994

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To examine the possible implication of protein SR, an VII-related antigen from Streptococcus mutans OMZ 175 (serotype f), in inflammatory reactions, we tested the immunomodulatory effects of protein SR on human monocytes. Using biotinylated protein, we provide evidence that protein SR binds to human monocytes in dose-, time-, and calcium-dependent manners through specific interactions. These results were confirmed by competition experiments using either soluble human monocyte extract or anti-SR immunoglobulin G. Binding occurred through lectin-like interactions between SR and carbohydrate portions of monocyte membrane glycoproteins, since binding could be inhibited by several sugars, especially fucose and N-acetylneuraminic acid (NANA), which were confirmed by ligand blotting to be the primer ligands recognized by SR on human monocyte extracts. The ability of protein SR to stimulate the production of cytokines by human circulating monocytes was then examined. The release of tumor necrosis factor alpha (TNF-a), interleukin 1p, and interleukin 6 is time and dose dependent and not affected by the addition of polymyxin B. Activation of monocytes resulted from specific binding of SR to NANA and fucose present on cell surface glycoproteins since TNF-a release could be inhibited by sialidase and pronase treatment of monocytes and by NANA and fucose. These results confirm that sialic acid and fucose present on cell surface macromolecules and especially glycoproteins are needed for the binding of SR to monocytes and for the release of TNF-a.

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“…Although the role of cytokines in the pathogenesis of dental caries is not distinct, proinflammatory cytokine production is induced by components of S. mutans. 7,8 The aim of this study was to assess association between IL-1α and IL-6 levels and dental caries, especially in S. mutans infections. In this study, 70 patients with dental caries were selected, who referred to the Department of Oral and Maxillary Surgery, Faculty of Dentistry, Tabriz University of Medical Sciences.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Relationship between Streptococcus mutans, Dental Caries and IL-1α and IL-6

Eslami

Pouralibaba

Sepas

et al. 2018

J Periodontol Implant Dent

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Background and aims. Streptococcus mutans is an important species in oral microflora and its components have been found to stimulate production of proinflammatory cytokines in dental caries. The aim of this study was to evaluate proinflammatory cytokines (IL-1α and IL-6) in patients with S. mutans. Materials and methods. Seventy samples were selected during pulpectomy and investigated for the presence of IL-1α and IL-6 by ELISA. The results were analyzed by t-test (α = 0.05). Results. The results showed higher mean concentrations of IL-6 and IL-1a in inflamed pulpal tissues in subjects with dental caries associated with S. mutans, compared with intact pulpal tissue samples; these higher means were statistically significant in all cases (P<0.05). Conclusion. The results of this study suggested relations between the production of IL1-a and IL-6 in dental caries caused by S. mutans.

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Activation of human monocytes by Streptococcus mutans serotype f polysaccharide: immunoglobulin G Fc receptor expression and tumor necrosis factor and interleukin-1 production

Cited by 34 publications

References 22 publications

Tumor necrosis factor‐α and interleukin‐1β expression pathway induced by Streptococcus mutans in macrophage cell line RAW 264.7

Tumor necrosis factor‐α and interleukin‐1β expression pathway induced by Streptococcus mutans in macrophage cell line RAW 264.7

Binding of Streptococcus mutans SR protein to human monocytes: production of tumor necrosis factor, interleukin 1, and interleukin 6

Evaluation of Relationship between Streptococcus mutans, Dental Caries and IL-1α and IL-6

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