Activation of Human Monocyte-Derived Macrophages Cultured on Teflon: Response to Interferon-γ during Terminal Maturation in vitro

Andreesen, Reinhard; Gadd, Stephen; Brugger, Wolfram; Löhr, G. W.; Atkins, Robert C.

doi:10.1016/s0171-2985(88)80038-x

Cited by 35 publications

(20 citation statements)

References 30 publications

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“…During this time, the cells increase in size (Fig. 1A) and develop features of tissue macrophages [23,241. In freshly isolated blood monocytes synthesis of BA4-amyloid precursor protein (APP) was nearly undetestable (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…derived macrophages, In the latter case, monocytes were incubated overmght and then removed from the dishes at 4°C by vigorous pipetring and placed, i_n_to rectangular teflon bags (Biofolie 25; Heraeus, Hanau. FRG) in supplemented RPMI 1640 medium eontainJn8 10°70 human AB serum, After g days, the monoeyt¢-derived maerophages exhibited features of typical tissue macrophages [23,24]. Cells were Volume 2~2, number 2…”

Section: 1 I-ironmentioning

confidence: 99%

“…matured human macrophages from purified peripheral blood monocytes which feature many of the characteristics of human microglia [21,22]. The in.vitro matured monocyte-derived macrophages share morphological and immunological features with microglia: compared to monocytes; they show a typical macrophage-like ruffled cell membrane under the electron microscope [21], and express surface antigens such as MHC class II molecules, receptors for Fc, C3, transferrin, and the CD4 receptor [23,24], In this report we show that monocyte-derived, in-vitro matured human macro. phages constitutively express APP mRNA and synthesize APP protein,…”

Section: Introductionmentioning

confidence: 99%

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In‐vitro matured human macrophages express Alzheimer's βA4‐amyloid precursor protein indicating synthesis in microglial cells

Bauer

König²,

Strauss

et al. 1991

FEBS Letters

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Micr~tllia which are consistenlly associatad with Al~hoimer's disease {AD) );chile plaques ~tre part of the monontJcle:tr phailocyte system, ln.vltro mmured human monocyte.d©rived m;~crophaltcs rc~t,rc many immunological characteristics of microlilia. We found stronll conslJtutive ©xpr0ss~on of' Atxheimcr's//A4.mnyioid precursor protein (APP) in human mononuc|ear pim$ocytes after terminal in.vitro maturation from monoc2ctes to macrophalleS, Amyloid has previously been found to b0 ~s~i.nted wilh microgli~t in AD brains, however, it remained unclear whctl~er the material was synthesized in or had been phagocytoscd by the ceils, The flndinlts presented here support the ~|ssumption that brain mlcroglia may contribute to APP synthesis in AD brain, Macrophage: Micr<)lilia; Arnyloid pr~:ursor protein; AIzheinner'~ disease

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Section: Resultsmentioning

confidence: 99%

Section: 1 I-ironmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In‐vitro matured human macrophages express Alzheimer's βA4‐amyloid precursor protein indicating synthesis in microglial cells

Bauer

König²,

Strauss

et al. 1991

FEBS Letters

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“…CDUis a myeloid differentiation antigen which is strongly expressed on the monocyte/macrophage lineage. Maturation ofmonocytes to macrophages is accompanied by an increased expression of CDI4 on the cell surface [6]. A soluble form of CD 14 (48 kD) has been detected in supematants of cultured CDI4' cells [7] as well as in serum and urine [8.9].…”

Section: Introductionmentioning

confidence: 99%

Elevated levels of soluble CD 14 in serum of patients with systemic lupus erythematosus

Nockher

Wigand

Schoeppe

et al. 1994

Clinical and Experimental Immunology

105

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SUMMARYA soluble fotin of CD!4 (sCD14) was assessed with an ELISA assay in Ihe serum of the following three clinical groups: 35 patients with ati inactive phase of systemie lupus erylhematosus (SLE). 17 patients with SLE relapses, and 65 normal healthy volunteers, Inereased levels of sCD14 were observed in all patients suffering from SLE compared with normal eontrols. In addition, patients with active SLE revealed higher serum concentrations of sCD14 (median 6-9 mg//) than patients under remission (41 mg//; P<() OO(i\). Serum values of sCDI4 correlated neither with the number of peripheral blood monocytes bearing the CD 14 membrane antigen, nor with serum concentrations of IL-1 p. Serum sCD 14 was compared with other clinical parameters used to monitor the clinical course of patients with SLE, among them complement C3. anti-dsDNA antibodies and soluble IL-2 receptor (sIL-2R). A good correlation emerged between sCD14 and C3 as well as sIL-2R eoneentrations. but sCD 14 and anti-dsDN A titres disclosed no significant correlation in both groups of patients with SLE. Serial studies in patients with severe SLE showed that serum sCD14 closely parallels the clinical course as defined by an activity score. Our data suggest that serum sCD14 represents a promising parameter lo monitor disease activity in patients with SLE.

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“…All three types of cytotoxicity depend on the differentiation stage of macrophages. Munn and Cheung (1989) have found that cultivation of monocytes with M-CSF increases ADCC and contact-dependent cytotoxicity against U937 increased during the maturation of monocytes into macrophages (Andreesen et al 1983b(Andreesen et al , 1988b. A soluble mediator of cytotoxicity, a newly described tumoricidal activity termed MCT-170, is secreted only by macrophages (Harwix et al 1992).…”

Section: Characterization Of Differentiation By Functional Activitymentioning

confidence: 99%

Differentiation of Human Monocytes In Vitro: A Model of Macrophage Ontogeny

Andreesen¹,

Kreutz²

1994

Cell Culture in Pharmaceutical Research

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Activation of Human Monocyte-Derived Macrophages Cultured on Teflon: Response to Interferon-γ during Terminal Maturation in vitro

Cited by 35 publications

References 30 publications

In‐vitro matured human macrophages express Alzheimer's βA4‐amyloid precursor protein indicating synthesis in microglial cells

In‐vitro matured human macrophages express Alzheimer's βA4‐amyloid precursor protein indicating synthesis in microglial cells

Elevated levels of soluble CD 14 in serum of patients with systemic lupus erythematosus

Differentiation of Human Monocytes In Vitro: A Model of Macrophage Ontogeny

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