Activation of gene expression by triplex-directed psoralen crosslinks

Song, Jie; Intody, Zsofia; Mei, Li; Wilson, John H.

doi:10.1016/j.gene.2003.09.037

Cited by 16 publications

(12 citation statements)

References 28 publications

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“…Song et al (112) demonstrated that psoralen can be used to activate gene expression up to 4-fold when targeted to sites upstream the promoter. These authors designed a psoralen–TFO adapter to deliver an artificial enhancer to a disabled gene in order to recruit a transcription factor and activate transcription.…”

Section: In Vitro Applications Of Tfos As Gene Modulatorsmentioning

confidence: 99%

“…In this case, the mutation introduced in the transcription factor binding site is directly responsible for gene activation. It is also possible to activate the transcription process when the triplex structure is formed in a repressor site, thus leading to a stimulation of the expression of the gene of interest (102,112). The activation of transcription has also been demonstrated in vivo with hairpin TFO in Saccharomyces cerevisiae (114).…”

Section: In Vitro Applications Of Tfos As Gene Modulatorsmentioning

confidence: 99%

See 1 more Smart Citation

The triple helix: 50 years later, the outcome

Duca

Vekhoff

Oussedik

et al. 2008

Nucleic Acids Research

317

269

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Triplex-forming oligonucleotides constitute an interesting DNA sequence-specific tool that can be used to target cleaving or cross-linking agents, transcription factors or nucleases to a chosen site on the DNA. They are not only used as biotechnological tools but also to induce modifications on DNA with the aim to control gene expression, such as by site-directed mutagenesis or DNA recombination. Here, we report the state of art of the triplex-based anti-gene strategy 50 years after the discovery of such a structure, and we show the importance of the actual applications and the main challenges that we still have ahead of us.

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Section: In Vitro Applications Of Tfos As Gene Modulatorsmentioning

confidence: 99%

Section: In Vitro Applications Of Tfos As Gene Modulatorsmentioning

confidence: 99%

The triple helix: 50 years later, the outcome

Duca

Vekhoff

Oussedik

et al. 2008

Nucleic Acids Research

317

269

View full text Add to dashboard Cite

show abstract

“…The plasmids carrying a single mutation at an Oct-1-binding site were introduced in MEL cells, leading to an increase in g-globin gene expression. A similar study has shown a 2-4-fold activation of GFP gene expression in various human cell lines (Song et al, 2004).…”

Section: Kolevzon and Yavinmentioning

confidence: 57%

Site-Specific DNA Photocleavage and Photomodulation by Oligonucleotide Conjugates

Kolevzon

Yavin

2010

Oligonucleotides

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Triplex-forming oligonucleotides have been explored in the last 3 decades as highly specific gene-modifying agents. Such agents have been showed to either upregulate or shut down gene expression in vitro, an outcome that depends on the specifically designed biological system. One interesting approach is to tether to the oligonucleotide a photoreactive moiety. After hybridization to the DNA target (typically single- or double-stranded DNA), a site-specific photoinduced reaction may take place at a timely manner. Further, as the light source may be focused at a certain area, one has control on the location at which the phototriggered DNA modification takes place. In this regard, the use of tissue-penetrating photons (typically 630-950 nm) would be most relevant for in vivo applications as photoactivation may lead to site-specific DNA modification at a specific tissue/organ. In this review we highlight the advances made in this field and discuss the hurdles that lay ahead for the realization of phototriggered triplex-forming oligonucleotides as a solid therapeutic approach.

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“…A practical approach in the treatment of certain genetic diseases may include increasing expression of genes that are transcribed at low levels. Song et al found that psoTFOs could activate gene expression on a plasmid by three to four fold [23]. Gene activation was found to be dependent upon psoralen photo-crosslinking.…”

Section: Tfo-mutagen Conugates Activate Gene Expressionmentioning

confidence: 99%

“…In fact, plasmids treated with the doubly modified TFO did not survive DNA replicational repair. Most likely the cells were not able to repair these adducts, and so the plasmids were eliminated [23]. Additionally, studies in both mammalian and yeast systems have shown that interstrand crosslinks are more mutagenic than monoadducts [31,32].…”

Section: Tfo-mutagen Conjugates Induce Muta-genesismentioning

confidence: 99%

Triplex-Forming Oligonucleotides as Potential Tools for Modulation of Gene Expression

Rogers¹,

Lloyd²,

Glazer³

2005

CMCACA

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Triplex-forming oligonucleotides (TFOs) bind in the major groove of duplex DNA at polypurine/ polypyrimidine stretches in a sequence-specific manner. The binding specificity of TFOs makes them potential candidates for use in directed genome modification. A number of studies have shown that TFOs can introduce permanent changes in a target sequence by stimulating a cell's inherent repair pathways. TFOs have also been demonstrated to inhibit gene expression providing a possible role for these compounds in cancer therapy. This review summarizes the dual roles of TFOs for use in delivering DNA reactive compounds to a specific site in the genome or for introducing permanent changes in the target sequence through the introduction of an altered helical structure. In addition to compiling the ways in which TFOs have been successfully utilized, this review will explore conflicting reports of TFO bioactivity focusing on the variables which affect the efficacy in vitro of TFO mediated genomic modification which in turn may represent the obstacles encountered using TFOs to modulate gene expression in vivo.

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Activation of gene expression by triplex-directed psoralen crosslinks

Cited by 16 publications

References 28 publications

The triple helix: 50 years later, the outcome

The triple helix: 50 years later, the outcome

Site-Specific DNA Photocleavage and Photomodulation by Oligonucleotide Conjugates

Triplex-Forming Oligonucleotides as Potential Tools for Modulation of Gene Expression

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