Activation of ethylene signaling is mediated by nuclear translocation of the cleaved EIN2 carboxyl terminus

Xing, Wen; Zhang, Cunli; Ji, Yusi; Zhao, Qiong; He, Wenrong; An, Fengying; Jiang, Liwen; Guo, Hongwei

doi:10.1038/cr.2012.145

Cited by 297 publications

(276 citation statements)

References 11 publications

(14 reference statements)

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“…We first demonstrated that EIN3 works downstream of EIN2 to advance leaf senescence, as observed in many other ethylene-regulated processes (Alonso et al, 1999;An et al, 2010;Wen et al, 2012). We then provided several lines of evidence to illustrate that EIN3 induces NAC2 expression by directly repressing miR164 transcription.…”

Section: The Emergence Of a Senescence Signaling Cascade Involving Eimentioning

confidence: 99%

“…EIN2 is a central signaling component required for all ethylene responses examined, as loss-of-function ein2 mutants show complete ethylene insensitivity (Alonso et al, 1999). EIN2 is shown to locate in the endoplasmic reticulum membrane (Bisson et al, 2009) and undergoes a hormone-induced cleavage and translocation event that is controlled by CONSTITUTIVE TRIPLE RESPONSE 1-directed phosphorylation of its C terminus (Ju et al, 2012;Qiao et al, 2012;Wen et al, 2012;Ji and Guo, 2013). In ethylene signaling, one of the key components working downstream of EIN2 is the EIN3 transcription factor (Chao et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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ETHYLENE-INSENSITIVE3 Is a Senescence-Associated Gene That Accelerates Age-Dependent Leaf Senescence by Directly Repressing miR164 Transcription in Arabidopsis

et al. 2013

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Numerous endogenous and environmental signals regulate the intricate and highly orchestrated process of plant senescence. Ethylene is a well-known inducer of senescence, including fruit ripening and flower and leaf senescence. However, the underlying molecular mechanism of ethylene-induced leaf senescence remains to be elucidated. Here, we examine ETHYLENE-INSENSITIVE3 (EIN3), a key transcription factor in ethylene signaling, and find that EIN3 is a functional senescence-associated gene. Constitutive overexpression or temporary activation of EIN3 is sufficient to accelerate leaf senescence symptoms. Conversely, loss of EIN3 and EIN3-Like1 (its close homolog) function leads to a delay in agedependent and ethylene-, jasmonic acid-, or dark-induced leaf senescence. We further found that EIN3 acts downstream of ORESARA2 (ORE2)/ORE3/EIN2 to repress miR164 transcription and upregulate the transcript levels of ORE1/NAC2, a target gene of miR164. EIN3 directly binds to the promoters of microRNA164 (miR164), and this binding activity progressively increases during leaf ageing. Genetic analysis revealed that overexpression of miR164 or knockout of ORE1/NAC2 represses EIN3-induced early-senescence phenotypes. Collectively, our study defines a continuation of the signaling pathway involving EIN2-EIN3-miR164-NAC2 in regulating leaf senescence and provides a mechanistic insight into how ethylene promotes the progression of leaf senescence in Arabidopsis thaliana.

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Section: The Emergence Of a Senescence Signaling Cascade Involving Eimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ETHYLENE-INSENSITIVE3 Is a Senescence-Associated Gene That Accelerates Age-Dependent Leaf Senescence by Directly Repressing miR164 Transcription in Arabidopsis

et al. 2013

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“…Both ethylene (ET) and jasmonate (JA) are essential plant hormones that regulate various plant developmental processes and diverse defense responses (Kieber, 1997;Bleecker and Kende, 2000;Guo and Ecker, 2004;Broekaert et al, 2006;Howe and Jander, 2008;Browse, 2009;Shan et al, 2012;Wasternack and Hause, 2013). ET signal is perceived by its receptors ETHYLENE RESPONSE1 (ETR1), ETR2, ETHYLENE RESPONSE SENSOR1 (ERS1), ERS2, and ETHYLENE INSENSITIVE4 (EIN4) (Hua and Meyerowitz, 1998) to repress CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) (Kieber et al, 1993), which activates EIN2 (Alonso et al, 1999;Ju et al, 2012;Qiao et al, 2012;Wen et al, 2012) and subsequently stabilizes EIN3 and EIN3-LIKE1 (EIL1) (Chao et al, 1997;Guo and Ecker, 2003;Potuschak et al, 2003;Gagne et al, 2004) to mediate various ET responses, including hypocotyl growth (Zhong et al, 2012), apical hook formation (Knight et al, 1910;An et al, 2012), root growth (Ortega-Martínez et al, 2007;Růzicka et al, 2007), flowering (Ogawara et al, 2003;Achard et al, 2007), fruit ripening (Burg and Burg, 1962;Theologis et al, 1992), leaf senescence (Gepstein and Thimann, 1981;Li et al, 2013), freezing tolerance , and resistance against pathogen infection (Alonso et al, 2003;Chen et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

et al. 2014

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Plants have evolved sophisticated mechanisms for integration of endogenous and exogenous signals to adapt to the changing environment. Both the phytohormones jasmonate (JA) and ethylene (ET) regulate plant growth, development, and defense. In addition to synergistic regulation of root hair development and resistance to necrotrophic fungi, JA and ET act antagonistically to regulate gene expression, apical hook curvature, and plant defense against insect attack. However, the molecular mechanism for such antagonism between JA and ET signaling remains unclear. Here, we demonstrate that interaction between the JA-activated transcription factor MYC2 and the ET-stabilized transcription factor ETHYLENE-INSENSITIVE3 (EIN3) modulates JA and ET signaling antagonism in Arabidopsis thaliana. MYC2 interacts with EIN3 to attenuate the transcriptional activity of EIN3 and repress ET-enhanced apical hook curvature. Conversely, EIN3 interacts with and represses MYC2 to inhibit JA-induced expression of wound-responsive genes and herbivory-inducible genes and to attenuate JA-regulated plant defense against generalist herbivores. Coordinated regulation of plant responses in both antagonistic and synergistic manners would help plants adapt to fluctuating environments.

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“…The volatile plant hormone ethylene (C 2 H 4 ) is involved in many cellular and developmental 42 processes, such as germination, root and hypocotyl development, climacteric fruit ripening, and 43 senescence (Abeles et al, 1992;Wen et al, 2015;Van de Poel et al, 2015), as well as in the response 44 to biotic and abiotic stress (Bari & Jones, 2009;Kazan et al, 2015). Photosynthesis is one of the least 45 referenced processes regulated by ethylene, which plays a role in all three carbon fixation pathways 46 used by plants: C3, C4, and crassulacean acid metabolism (CAM) (Box 1).…”

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confidence: 99%

Ethylene Exerts Species-Specific and Age-Dependent Control of Photosynthesis

Ceusters

Poel

2018

Plant Physiol.

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31The volatile plant hormone ethylene plays a regulatory role in many developmental processes and in 32 biotic and abiotic stress responses. One of the under-explored actions of ethylene is its regulation of 33 photosynthesis and associated components such as stomatal conductance, chlorophyll content, light 34 reactions, carboxylation events, carbohydrate partitioning, and age-related senescence. In this 35 update, we summarize the current knowledge concerning the regulation of photosynthesis, focusing 36 on the model species Arabidopsis thaliana. We describe how ethylene directs photosynthesis in 37 juvenile non-senescing leaves and mature senescing leaves. Furthermore, we extend these insights 38 Plant Physiology Preview.

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Activation of ethylene signaling is mediated by nuclear translocation of the cleaved EIN2 carboxyl terminus

Cited by 297 publications

References 11 publications

ETHYLENE-INSENSITIVE3 Is a Senescence-Associated Gene That Accelerates Age-Dependent Leaf Senescence by Directly Repressing miR164 Transcription in Arabidopsis

ETHYLENE-INSENSITIVE3 Is a Senescence-Associated Gene That Accelerates Age-Dependent Leaf Senescence by Directly Repressing miR164 Transcription in Arabidopsis

Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

Ethylene Exerts Species-Specific and Age-Dependent Control of Photosynthesis

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