Activation of Ethanol Production by Combination of  Recombinant &amp;lt;i&amp;gt;Ralstonia eutropha&amp;lt;/i&amp;gt; and Electrochemical  Reducing Power

Jeon, Bo Young; Yi, Jun; Jung, Il Lae; Park, Doo Hyun

doi:10.4236/aim.2013.31006

Cited by 10 publications

(5 citation statements)

References 18 publications

(28 reference statements)

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“…Moreover, it was reported that PHB producing strains have the ability to balance the reducing equivalents generated from carbon metabolism by using PHB as a sink for reducing power (i.e., NADPH) [ 26 , 27 ]. Additionally, fermentative bacteria (grown on CO 2 and H 2 ) with high reducing power (NADH) favor metabolite production over cell growth [ 28 ]. In opposite to these expectations, the control experiments with introducing hydrogen did not show any PHB production.…”

Section: Discussionmentioning

confidence: 99%

Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

et al. 2018

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Poly(3-hydroxybutyrate) (PHB), a biodegradable polymer, can be produced by different microorganisms. The PHB belongs to the family of polyhydroxyalkanoate (PHA) that mostly accumulates as a granule in the cytoplasm of microorganisms to store carbon and energy. In this study, we established an integrated one-pot electromicrobial setup in which carbon dioxide is reduced to formate electrochemically, followed by sequential microbial conversion into PHB, using the two model strains, Methylobacterium extorquens AM1 and Cupriavidus necator H16. This setup allows to investigate the influence of different stress conditions, such as coexisting electrolysis, relatively high salinity, nutrient limitation, and starvation, on the production of PHB. The overall PHB production efficiency was analyzed in reasonably short reaction cycles typically as short as 8 h. As a result, the PHB formation was detected with C. necator H16 as a biocatalyst only when the electrolysis was operated in the same solution. The specificity of the source of PHB production is discussed, such as salinity, electricity, concurrent hydrogen production, and the possible involvement of reactive oxygen species (ROS).

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Section: Discussionmentioning

confidence: 99%

Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

et al. 2018

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“…Since then, cathodically reduced neutral red has been shown to drive fumarate reduction in Actinobacillus succinogenes (Park and Zeikus, 1999), act as the sole electron donor for growth and metabolite formation (Park et al, 1999), generate a proton motive force in combination with a proton-translocating fumarate reductase (Park and Zeikus, 1999), chemically reduce NAD + (Park and Zeikus, 1999), enhance ethanol production in Clostridium thermocellum and Saccharomyces cerevisiae (Shin et al, 2002), enhance reduced pathway flux in Weissella kimchii (Park et al, 2005), modify carbon flow in Clostridium acetobutylicum (Girbal et al, 2006), and when immobilized on a graphite electrode, enhance ethanol formation in Zymomonas mobilis (Jeon et al, 2009; Jeon and Park, 2010) and Ralstonia Eutropha (Jeon et al, 2013). There is confusion from some of these reports where high voltage drops were involved, which may have produced hydrogen via electrolysis in addition to reducing neutral red.…”

Section: Introductionmentioning

confidence: 99%

“…There is confusion from some of these reports where high voltage drops were involved, which may have produced hydrogen via electrolysis in addition to reducing neutral red. Some of these voltage drops include: 2 V (Jeon et al, 2012), 3 V (Jeon et al, 2009, 2013), and 1-10 V variable (Jeon and Park, 2010; Shin et al, 2002). Very few studies employed the use of a potentiostat to monitor and control working electrode potential while simultaneously quantifying charge transfer in order to compare electron delivery with metabolite production, with at least one recent exception (Choi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Neutral red-mediated microbial electrosynthesis by Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis

Harrington

Mohamed

Tran

et al. 2015

Bioresource Technology

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The aim of this work was to compare the effects of electrosynthesis on different bacterial species. The effects of neutral red-mediated electrosynthesis on the metabolite profiles of three microorganisms: Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis, were measured and compared and contrasted. A statistically comprehensive analysis of neutral red-mediated electrosynthesis is presented using the analysis of end-product profiles, current delivered, and changes in cellular protein expression. K. pneumoniae displayed the most dramatic response to electrosynthesis of the three bacteria, producing 93% more ethanol and 76% more lactate vs. control fermentation with no neutral red and no electron delivery. Z. mobilis showed no response to electrosynthesis except elevated acetate titers. Stoichiometric comparison showed that NAD+ reduction by neutral red could not account for changes in metabolites during electrosynthesis. Neutral red-mediated electrosynthesis was shown to have multifarious effects on the three species.

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“…Electricity generated from regenerative energies sources can be stored in form of electromicrobial fuels. Recombinant strains of R. eutropha were used electromicrobial conversion of CO2/formate to higher alcohols (47,65). These auto-and mixotrophical approaches comprise, on one hand, the possibility to store energy and on the other, consume CO2, which is also beneficial for the environment.…”

Section: Utilization Of C1-compoundsmentioning

confidence: 99%

Engineering the heterotrophic carbon sources utilization range ofRalstonia eutrophaH16 for applications in biotechnology

Володина

Raberg

Steinbüchel

2015

Critical Reviews in Biotechnology

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Ralstonia eutropha H16 is an interesting candidate for the biotechnological production of polyesters consisting of hydroxy- and mercaptoalkanoates, and other compounds. It provides all the necessary characteristics, which are required for a biotechnological production strain. Due to its metabolic versatility, it can convert a broad range of renewable heterotrophic resources into diverse valuable compounds. High cell density fermentations of the non-pathogenic R. eutropha can be easily performed. Furthermore, this bacterium is accessible to engineering of its metabolism by genetic approaches having available a large repertoire of genetic tools. Since the complete genome sequence of R. eutropha H16 has become available, a variety of transcriptome, proteome and metabolome studies provided valuable data elucidating its complex metabolism and allowing a systematic biology approach. However, high production costs for bacterial large-scale production of biomass and biotechnologically valuable products are still an economic challenge. The application of inexpensive raw materials could significantly reduce the expenses. Therefore, the conversion of diverse substrates to polyhydroxyalkanoates by R. eutropha was steadily improved by optimization of cultivation conditions, mutagenesis and metabolic engineering. Industrial by-products and residual compounds like glycerol, and substrates containing high carbon content per weight like palm, soybean, corn oils as well as raw sugar-rich materials like molasses, starch and lignocellulose, are the most promising renewable substrates and were intensively studied.

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Activation of Ethanol Production by Combination of Recombinant <i>Ralstonia eutropha</i> and Electrochemical Reducing Power

Cited by 10 publications

References 18 publications

Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

Neutral red-mediated microbial electrosynthesis by Escherichia coli, Klebsiella pneumoniae, and Zymomonas mobilis

Engineering the heterotrophic carbon sources utilization range ofRalstonia eutrophaH16 for applications in biotechnology

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