Activation of eNOS by D-pinitol Induces an Endothelium-Dependent Vasodilatation in Mouse Mesenteric Artery

Moreira, Luciana N.; Silva, Josiane F.; Silva, Grazielle C.; Lemos, Virgı́nia S.; Côrtes, Steyner F.

doi:10.3389/fphar.2018.00528

Cited by 14 publications

(8 citation statements)

References 45 publications

(57 reference statements)

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“…Our results are in agreement with previous studies that have shown the involvement of the BK Ca2+ channels in the endothelium-independent vasodilation of the flavonoids quercetin, puerarin, naringenin, dioclein, and luteolin, and for the non-flavonoid phenol resveratrol [31][32][33][34][35][36]. Therefore, activation of BK Ca2+ channels seems to be a key mechanism that might account for a good portion of the observed phenol-induced vasodilation.…”

Section: Discussionsupporting

confidence: 93%

Extra Virgin Olive Oil Phenols Dilate the Rat Mesenteric Artery by Activation of BKCa2+ Channels in Smooth Muscle Cells

et al. 2020

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Accumulating evidence has shown the beneficial health effects of extra virgin olive oil (EVOO) consumption in reducing blood pressure and preventing the risk of developing hypertension. Some studies associate the hypotensive activity of EVOO to a minor component—the phenols. This study was designed to investigate the effects of EVOO phenols on the rat resistance mesenteric artery (MA) and to find out the possible vascular pathways involved. The experiments were carried out using a pressurized myograph, which allowed the effects of phenols on isolated MA to be tested under different conditions: (a) with endothelium removed; (b) with inhibition of nitric oxide synthase by Nω-Nitro-l-arginine methyl ester hydrochloride (l-NAME, 10−4 M) + Nω-Nitro-l-arginine (l-NNA, 10−4 M); (c) with inhibition of cyclooxygenase by indomethacin (10−5 M); (d) with inhibition of guanylate cyclase by 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ,10−5 M) or adenylate cyclase by 9-(Tetrahydro-2′-furyl)adenine (SQ, 10−5 M); (e) with depolarization by high potassium chloride (40 mM); and (f) with inhibition of the large conductance Ca2+–potassium channels (BKCa2+) with paxilline (10−5 M). EVOO phenols induce vasodilation of the endothelium, mediated by a direct effect on smooth muscle cells (SMC) by activation of BKCa2+ channels, an action by which phenols can regulate the vascular tone of the resistance artery. Phenols can be regarded as bioactive molecules that may contribute to the antihypertensive effects of EVOO.

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Section: Discussionsupporting

confidence: 93%

Extra Virgin Olive Oil Phenols Dilate the Rat Mesenteric Artery by Activation of BKCa2+ Channels in Smooth Muscle Cells

et al. 2020

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“…Further-more, it treats diabetes by lowering blood glucose levels in people with type 2 diabetes (T2DM) and reduces the metabolic parameters associated with cardiovascular risk [17]. The protective effect of DP against diabetic-induced endothelial dysfunctions of mesenteric artery is attributed to its antioxidant activity [18]. In this study, we observed that the glucose level and HW/BW in the diabetic mice were significantly decreased by the administration of DP.…”

Section: Discussionmentioning

confidence: 69%

Phosphoproteomics analysis of diabetic cardiomyopathy in aging‐accelerated mice and effects of D‐pinitol

et al. 2021

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Purpose:The molecular mechanisms of diabetic cardiomyopathy (DCM) development and D-pinitol (DP) in its treatment remain unclear. The present study is to explore the underlying mechanism of DCM in an elderly diabetic mouse model and to seek the protective targets of DP by phosphoproteomics. Experimental design:We used streptozotocin to induce diabetes in SAMP8 and DP (150 mg/kg/day) intragastrically administrated to diabetic mice for 8 weeks. The heart tissues were harvested for label-free phosphoproteomic analysis from diabetic mice. Some differentially regulated phosphorylation sites were confirmed by parallel reaction monitoring.Results: Our results showed that 612 phosphorylation sites on 454 proteins had their phosphorylation levels significantly changed in the heart of untreated diabetic mice (DM). Of these phosphorylation sites, 216 phosphorylation sites on 182 proteins were normalized after DP treatment. We analyzed the functional signaling pathways in the heart of DP treated diabetic mice (DMT), including glucagon signaling pathway, insulin signaling pathway, mitophagy, apoptosis, and longevity regulating pathway. Two consensus motifs identified were targeted by Src and epidermal growth factor receptor between DMT and DM groups. Conclusions and clinical relevance:Our study might help to better understand the mechanism of DCM, provide novel targets for estimating the protective effects of DP.

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“…Additionally, the administration of a high dose of MI to diabetic monkeys was also effective in reducing blood glucose levels [58]. Recent studies have shown that the oral administration of MI is able to improve glucose tolerance and induce to GLUT-4 translocation in skeletal muscles in C57/BL6 mice, suggesting improvement of insulin sensitivity by inositol (Table 1) [20,59].…”

Section: Cyclitols In Treatmentmentioning

confidence: 99%

Health-Promoting Properties of Selected Cyclitols for Metabolic Syndrome and Diabetes

et al. 2019

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Cyclitols play a particularly important role in cell functioning because they are involved in ion channel physiology, phosphate storage, signal transduction, cell wall formation, membrane biogenesis, osmoregulation and they have antioxidant activity. They are involved in the cell membranes as a phosphatidyl myo-inositol, an inositol triphosphate precursor, which acts as a transmitter that regulates the activity of several hormones, such as follicle-stimulating hormone, thyrotropin, and insulin. The aim of this paper is to characterize the selected cyclitols: myo-inositol, D-chiro-inositol, and D-pinitol in type-2 metabolic syndrome and diabetes treatment. Results and discussion: Cyclitols have certain clinical applications in the treatment of metabolic syndromes and are considered to be an option as a dietary supplement for the treatment or prevention of gestational diabetes mellitus and type-2 diabetes. Improved metabolic parameters observed after using cyclitols, like myo-inositol, in the treatment of polycystic ovary syndrome and type-2 diabetes suggest that they may have a protective effect on the cardiovascular system. Pinitol, together with myo-inositol,maybe responsible for improving lipid profiles by reducing serum triglyceride and total cholesterol. Pinitol is also well-researched and documented for insulin-like effects. Myo-inositol, D-chiro-inositol, and D-pinitol indicate a number of therapeutic and health-promoting properties.

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Activation of eNOS by D-pinitol Induces an Endothelium-Dependent Vasodilatation in Mouse Mesenteric Artery

Cited by 14 publications

References 45 publications

Extra Virgin Olive Oil Phenols Dilate the Rat Mesenteric Artery by Activation of BKCa2+ Channels in Smooth Muscle Cells

Extra Virgin Olive Oil Phenols Dilate the Rat Mesenteric Artery by Activation of BKCa2+ Channels in Smooth Muscle Cells

Phosphoproteomics analysis of diabetic cardiomyopathy in aging‐accelerated mice and effects of D‐pinitol

Health-Promoting Properties of Selected Cyclitols for Metabolic Syndrome and Diabetes

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