Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121 in apple

He, Xin-Qiang; Huo, Yanhong; Liu, Xiuxia; Zhou, Qianqian; Feng, Shouqian; Shen, Xiang; Li, Baohua; Wu, Shu-Jing; Chen, Xuesen

doi:10.1038/s41438-018-0030-5

Cited by 43 publications

(26 citation statements)

References 51 publications

(74 reference statements)

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“…ICS is required for the synthesis of SA (Métraux 2002). B. dothidea is one of the important pathogens causing apple ring rot (He et al 2018). However, it is unclear if ICS is involved in the resistance to B. dothidea in the SA biosynthesis pathway.…”

Section: Botryosphaeria Dothidea Induces Expression Of Mdics1 and Mdics2mentioning

confidence: 99%

“…The SNARE protein syntaxin 121 (SYP121) had an effect on resistance to B. dothidea by affecting the oxidative‐reductive process in apple. After silencing MdSYP121 in apple calli, the resistance to apple ring rot was enhanced by regulating the SA signaling pathway and the oxidative‐reductive process (He et al 2018). MdPR‐4 gene expression can be induced by salicylic acid (SA) and B. dothidea infection, and it can inhibit the growth of B. dothidea hyphae to resist the invasion of this pathogen and to improve the resistance to B. dothidea (Bai et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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MdWRKY15 improves resistance of apple to Botryosphaeria dothidea via the salicylic acid‐mediated pathway by directly binding the MdICS1 promoter

Zhao

Jiang

et al. 2019

JIPB

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Isochorismate synthase (ICS) plays an essential role in the accumulation of salicylic acid (SA) and plant disease resistance. Diseases caused by Botryosphaeria dothidea affect apple yields. Thus, it is important to understand the role of ICS1 in disease resistance to B. dothidea in apple. In this study, SA treatment enhanced the resistance to B. dothidea. MdICS1 was induced by B. dothidea and enhanced the resistance to B. dothidea. MdICS1 promoter analysis indicated that the W‐box was vital for the response to B. dothidea treatment. MdWRKY15 was found to interact with the W‐box using yeast one‐hybrid screening. Subsequently, the interaction was confirmed by EMSA, yeast one‐hybrid, ChIP‐PCR, and quantitative PCR assays. Moreover, luciferase and GUS analysis further indicated that MdICS1 was transcriptionally activated by MdWRKY15. Finally, we found the function of MdWRKY15 in the resistance to B. dothidea was partially dependent on MdICS1 from the phenotype of transgenic apples and calli. In summary, we revealed that MdWRKY15 activated the transcription of MdICS1 by directly binding to its promoter to increase the accumulation of SA and the expression of disease‐related genes, thereby resulting in the enhanced resistance to B. dothidea in the SA biosynthesis pathway.

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Section: Botryosphaeria Dothidea Induces Expression Of Mdics1 and Mdics2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MdWRKY15 improves resistance of apple to Botryosphaeria dothidea via the salicylic acid‐mediated pathway by directly binding the MdICS1 promoter

Zhao

Jiang

et al. 2019

JIPB

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“…Some genes associated with host resistance to B. dothidea have been reported. It was found that resistance to B. dothidea is affected by E3 ubiquitin ligases, ethylene-response factor, and the salicylic acid signaling pathway (He et al, 2018;Han et al, 2019;Zhao et al, 2019). Although the CNGC family has been well-studied in several model plants, including Arabidopsis, rice, and tomato, apple CNGCs have seldom been reported.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Mutagenesis of MdCNGC2 in Apple Callus and VIGS-Mediated Silencing of MdCNGC2 in Fruits Improve Resistance to Botryosphaeria dothidea

et al. 2020

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“…A peroxiredoxin of defense response to bacterium was stimulated 549 at 48 hai, possibly as a result of the presence of ROS, because it is a sensor peroxidase 550 [81]. Amine oxidases collaborate in ROS production and are upregulated in resistant 551 apple and peach plants submitted to biotic stress [34,121]. These genes formed a large 552 group characterized by repression at 12 hai, with some members showing an LFC less 553 than -2.0 until 24 hai (S4 File-E).…”

mentioning

confidence: 99%

Time-series expression profiling of sugarcane leaves infected withPuccinia kuehniireveals an ineffective defense system leading to susceptibility

Correr

Hosaka

Gomez

et al. 2019

Preprint

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Puccinia kuehnii is an obligate biotrophic fungus that infects sugarcane leaves causing a disease called orange rust. It spread out to other countries resulting in reduction of crop yield since its first outbreak in Australia. One of the knowledge gaps of that pathosystem is to understand the molecular mechanisms altered in susceptible plants by the stress induced by P. kuehnii. Here we investigated changes in temporal expression of transcripts in pathways associated with the immune system. To achieve this purpose, we used RNA-Seq to analyze infected leaf samples collected at 0, 12, 24, 48 hours after inoculation (hai), 5 and 12 days after inoculation (dai). A de novo transcriptome was assembled with preprocessed reads, filtering out potential fungal sequences to focus on plant transcripts. Differential expression analyses of adjacent time points revealed substantial changes at 12, 48 hai and 12 dai, coinciding with the events of spore germination, haustoria post-penetration and post-sporulation. During the first 24 hours, a lack of transcripts involved with resistance mechanisms was revealed by underrepresentation of hypersensitive and defense responses. However, two days after inoculation, upregulation of genes involved with immune response regulation provided evidence of some potential defense response. Events related to biotic stress and phenylpropanoid biosynthesis pathways were predominantly downregulated in the initial time points, but expression was later restored to basal levels. Similar waves of expression were apparent for sets of genes in photosynthesis and oxidative processes, whose initial repression could avoid production of signaling molecules. Their subsequent upregulation possibly restored carbohydrate metabolism, ensuring pathogen nutritional requirements were met. Our results support the hypothesis that P. kuehnii initially suppressed sugarcane genes involved in plant defense systems. Late overexpression of specific regulatory pathways also suggests the possibility of an inefficient recognition system by a susceptible sugarcane genotype. March 15, 2019 1/27 1 Sugarcane is currently cultivated in 27 million hectares worldwide, 52% of which are 2 located in America, more expressively in South America (11 million ha). Brazil is the 3 main producer, responsible for 768.68 million tonnes (FAOSTAT, 2016). The term 4 sugarcane includes at least six species of the genus Saccharum [1, 2]. The ancestor 5 species are Saccharum robustum and S. spontaneum, which present variable ploidy 6 levels with chromosome number ranges of 2n = 60 -170 and 2n = 36 -128, respectively 7 [3, 4]. The octoploid (2n = 80) S. officinarum is an ancient cultivated sugarcane with 8 high sugar content that formed the group of noble cultivars [3, 5, 6]. Sugarcane plants 9 currently used for industrial purposes are modern cultivars derived from interspecific 10 crosses, mainly between S. officinarum and S. spontaneum. As a consequence of 11 successive backcrosses, the majority of chromosomes in homology groups are from S. 12 offici...

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Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121 in apple

Cited by 43 publications

References 51 publications

MdWRKY15 improves resistance of apple to Botryosphaeria dothidea via the salicylic acid‐mediated pathway by directly binding the MdICS1 promoter

MdWRKY15 improves resistance of apple to Botryosphaeria dothidea via the salicylic acid‐mediated pathway by directly binding the MdICS1 promoter

CRISPR/Cas9-Mediated Mutagenesis of MdCNGC2 in Apple Callus and VIGS-Mediated Silencing of MdCNGC2 in Fruits Improve Resistance to Botryosphaeria dothidea

Time-series expression profiling of sugarcane leaves infected withPuccinia kuehniireveals an ineffective defense system leading to susceptibility

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