Activation of cell migration via morphological changes in focal adhesions depends on shear stress in MYCN-amplified neuroblastoma cells

Hiraiwa, Takumi; Yamada, Takahiro; Miki, Norihisa; Funahashi, Akira; Hiroi, Noriko

doi:10.1098/rsif.2018.0934

Cited by 6 publications

(4 citation statements)

References 57 publications

(84 reference statements)

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“…Cells formed a stable attachment to the PDMS and nanopillar surfaces over time, and thus the aspect ratio increased with time. However, there was more frequent cytoskeletal rearrangements in cells on nanopillars than in cells on flat PDMS surfaces, which corresponded to more rapid cell movements, as shown in Supplementary movie SV 1 (b) 42 . In addition, the aspect ratio for cells on the silicon oxide surface remained the smallest for 16 h. With FN coating, the protein layer facilitated cell spreading in multiple directions, leading to a more rounded cell shape.…”

Section: Resultsmentioning

confidence: 95%

Effects of nanopillars and surface coating on dynamic traction force

Cheng

Pang

2023

Microsyst Nanoeng

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The extracellular matrix serves as structural support for cells and provides biophysical and biochemical cues for cell migration. Topography, material, and surface energy can regulate cell migration behaviors. Here, the responses of MC3T3-E1 cells, including migration speed, morphology, and spreading on various platform surfaces, were investigated. Polydimethylsiloxane (PDMS) micropost sensing platforms with nanopillars, silicon oxide, and titanium oxide on top of the microposts were fabricated, and the dynamic cell traction force during migration was monitored. The relationships between various platform surfaces, migration behaviors, and cell traction forces were studied. Compared with the flat PDMS surface, cells on silicon oxide and titanium oxide surfaces showed reduced mobility and less elongation. On the other hand, cells on the nanopillar surface showed more elongation and a higher migration speed than cells on silicon oxide and titanium oxide surfaces. MC3T3-E1 cells on microposts with nanopillars exerted a larger traction force than those on flat PDMS microposts and had more filopodia and long protrusions. Understanding the relationships between platform surface condition, migration behavior, and cell traction force can potentially lead to better control of cell migration in biomaterials capable of promoting tissue repair and regeneration.

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Section: Resultsmentioning

confidence: 95%

Effects of nanopillars and surface coating on dynamic traction force

Cheng

Pang

2023

Microsyst Nanoeng

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“…However, this observation was contradictory to a previous study indicating that LSS causes inhibition of the migration of bovine aortic endothelial cell as compared to static condition while HSS increases the speed of migration of these cells [ 52 ]. Similarly, reports have also indicated that HSS increases the migration speed and directional migration of neuroblastoma IMR32 cells via regulating the MYCN protein [ 53 ]. Thus, it seems that the effect of HSS on cell migration may be controversial and may be depends on cell types, cell origin and the level of SS applied.…”

Section: Discussionmentioning

confidence: 99%

Cholesterol efflux regulator ABCA1 exerts protective role against high shear stress-induced injury of HBMECs via regulating PI3K/Akt/eNOS signaling

et al. 2022

BMC Neurosci

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Background In brain, microvascular endothelial cells are exposed to various forces, including shear stress (SS). However, little is known about the effects of high shear stress (HSS) on human brain microvascular endothelial cells (HBMECs) and the underlying mechanism. The cholesterol efflux regulator ATP-binding cassette subfamily A member 1 (ABCA1) has been demonstrated to exert protective effect on HBMECs. However, whether ABCA1 is involved in the mechanism underneath the effect of HSS on HBMECs remains obscure. In the present study, a series of experiments were performed to better understand the effect of HSS on cellular processes of HBMECs and the possible involvement of ABCA1 and PI3K/Akt/eNOS in the underlying mechanisms. Results HBMECs were subjected to physiological SS (PSS) or high SS (HSS). Cell migration was evaluated using Transwell assay. Apoptotic HBMECs were detected by flow cytometry or caspase3/7 activity. IL-1β, IL-6, MCP-1 and TNF-α levels were measured by ELISA. RT-qPCR and western blotting were used for mRNA and protein expression detection, respectively. ROS and NO levels were detected using specific detection kits. Compared to PSS, HBMECs exhibited decreased cell viability and migration and increased cell apoptosis, increased levels of inflammatory cytokines, and improved ROS and NO productions after HSS treatment. Moreover, HSS downregulated ABCA1 but upregulated the cholesterol efflux-related proteins MMP9, AQP4, and CYP46 and activated PI3K/Akt/eNOS pathway. Overexpression of ABCA1 in HBMECS inhibited PI3K/Akt/eNOS pathway and counteracted the deleterious effects of HSS. Contrary effects were observed by ABCA1 silencing. Inhibiting PI3K/Akt/eNOS pathway mimicked ABCA1 effects, suggesting that ABCA1 protects HBMECs from HSS via PI3K/Akt/eNOS signaling. Conclusion These results advanced our understanding on the mechanisms of HSS on HBMECs and potentiated ABCA1/PI3K/Akt/eNOS pathway as therapeutic target for cerebrovascular diseases.

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“…However, cells had a smaller aspect ratio but frequent cytoskeletal rearrangement compared to those on at PDMS surface, corresponding to more rapid cell movement, as shown in Supplementary movie SV1(b). 43 In addition, the aspect ratio for cells on titanium oxide surface was larger than those on silicon oxide surface.…”

Section: Cell Migration and Cell Morphology On Different Surfacesmentioning

confidence: 99%

Effects of Nanopillars and Surface Coating on Dynamic Traction Force

Pang

Cheng

2022

Preprint

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Extracellular matrix serves as a structural support for cells and provides biophysical and biochemical cues for cell migration. Topography, material, and surface energy can regulate cell migration behaviors. The responses of MC3T3-E1 cells, including migration speed, morphology, and spreading on various platform surfaces were investigated. Polydimethylsiloxane (PDMS) micropost sensing platforms with nanopillars, silicon oxide, and titanium oxide on top of the microposts were fabricated, and dynamic cell traction force during migration was monitored. The relationships between various platform surfaces, migration behaviors, and cell traction force were studied. Compared with flat PDMS surface, cells on silicon oxide and titanium oxide surfaces showed reduced mobility, less elongation, and larger cell area. On the other hand, cells on nanopillar surface showed more elongation, higher migration speed, and smaller cell area compared to cells on silicon oxide and titanium oxide surfaces. MC3T3-E1 cells on microposts with nanopillars exerted larger traction force than those on microposts without nanopillars, as well as having more extensions of filopodia and long protrusions. Understanding the relationships between platform surface condition, migration behavior, and cell traction force can potentially lead to better control of cell migration in biomaterials capable of promoting tissue repair and regeneration.

show abstract

Activation of cell migration via morphological changes in focal adhesions depends on shear stress in MYCN-amplified neuroblastoma cells

Cited by 6 publications

References 57 publications

Effects of nanopillars and surface coating on dynamic traction force

Effects of nanopillars and surface coating on dynamic traction force

Cholesterol efflux regulator ABCA1 exerts protective role against high shear stress-induced injury of HBMECs via regulating PI3K/Akt/eNOS signaling

Effects of Nanopillars and Surface Coating on Dynamic Traction Force

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