Activation of Bone Marrow Multipotent Stromal Cells by Laser and EHF Radiation and Their Combined Impacts

Chailakhyan, R. K.; Gerasimov, Yu. V.; Yusupov, V. I.; Sviridov, Аlexander P.; Тамбиев, А.Х.; Vorobieva, N. N.; Grosheva, A. G.; Куралесова, А. И.; Moskvina, I. L.; Баграташвили, В. Н.

doi:10.17691/stm2017.9.1.03

Cited by 9 publications

(9 citation statements)

References 5 publications

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“…In AILIH+TEC group, this parameter was 6-fold higher than in AILIH group and by 1.3 times higher than in the control level, but by 1.7 times lower than in EHF+AILIH group. These fi ndings confi rmed and coincided with the previous data (in vitro) [6,7], which show an increase in the proliferative activity of MSC after exposure to AILIH and EHF+AILIH (by 47 and 58%, respectively). Thus, the ratio of MSC number in 3-and 5-month transplants in study groups was similar.…”

Section: Resultssupporting

confidence: 91%

“…Exposure of precipitated hemopoietic cells to AILIH [6,[7][8][9]12,13] was performed using a piezoceramic transformer in a working chamber of AILIH instrument fi lled with water [7]. The tube with precipitated or placed into a sponge bone marrow cells was placed into working cuvette of the apparatus and exposed to AILIH for 50 sec.…”

Section: Methodsmentioning

confidence: 99%

“…Here we studied in vivo effects of acoustic impulses of laser-induced hydrodynamics (AILIH) [6,[7][8][9]12,13] and EHF-radiation on the formation and size of organ containing bone marrow and hemopoietic tissues.…”

mentioning

confidence: 99%

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Effects of Acoustic and EHF Impulses on Multipotent Stromal Cells during Formation of Bone Marrow Containing Heterotopic Organs in Tissue Engineered Constructions

et al. 2015

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We studied the effects of physical factors (acoustic impulses of laser-induced hydrodynamics, AILIH, and EHF-radiation) on the formation of heterotopic bone marrow organs. Suspension of precipitated mouse bone marrow cells was exposed to AILIH and EHF or their combinations (AILIH+EHF, EHF+AILIH). The developed tissue engineering constructions (gelatin sponges containing 107 nucleated bone marrow cells exposed to physical factors) were transplanted under the renal capsule of syngeneic mice. Analysis of newly formed hemopoietic organs was performed after 3 and 5 months. The total amount of hemopoietic cells, number of multipotent stromal cells, efficiency of colony formation from these cells, and weight of bone capsule of the transplants were measured. Microscopic study showed that 5-month transplants were significantly larger than 3-month transplants and contained 3-fold more hemopoietic cells (20-fold in the AILIH+EHF group). The number of multipotent stromal cells was maximum in EHF+AILIH group (by 2.2 times higher than in the control) and minimum in AILIH+EHF group. Exposure to EHF+AILIH had most pronounced effect on the formation of the bone marrow transplants. The weight of bone capsules more rapidly increased in gelatin sponges of 3-month transplants of EHF+AILIH and AILIH groups. These data suggest that the studied physical factors can be used for acceleration of rehabilitation process.

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Section: Resultssupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

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Effects of Acoustic and EHF Impulses on Multipotent Stromal Cells during Formation of Bone Marrow Containing Heterotopic Organs in Tissue Engineered Constructions

et al. 2015

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“…It was shown that such sound could provide a protective e®ect on stem cells. 20 The stimulating e®ect of the laser with SAC¯ber tip at 3 W for 20 s is probably due to broadband sound generation. Acoustic noise from the proposed system was studied in papers.…”

Section: Discussionmentioning

confidence: 99%

The bactericidal effect of continuous wave laser with strongly absorbing coating at the fiber tip

Elagin

Smirnov

Yusupov

et al. 2018

J. Innov. Opt. Health Sci.

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The bactericidal effect of laser radiation with a quartz fiber-based transmission system with a strong absorption coating converter against bacteria associated with urological stones has been studied. Gram-negative rod Escherichia coli and the Gram-positive coccus Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium were used in this study. Each bacterial species was treated by continuous-wave near infrared laser coupled with bare fiber tip or strongly absorption coating fiber tip. After treatment, the temperature of bacterial suspension was measured. In addition, the temperature distribution was analyzed. It has been shown that using laser with a strongly absorption coating fiber tip results in significant bactericidal effect. The decrease of the amount of E. coli and S. epidermidis was 100% after treatment with an output power of 6[Formula: see text]W of radiation at a wavelength of 0.97[Formula: see text][Formula: see text]m for 40[Formula: see text]s. Number of S. aureus and Ent. faecium colony-forming unit was reduced to 70% after same exposure. The peak temperature of bacterial suspension was [Formula: see text]C after treatment by laser with a strongly absorption coating fiber tip. Laser with a strongly absorption coating fiber tip provides large-scale hydrodynamic flows directed away from the fiber tip. The laser with a strongly absorption coating fiber tip has bactericidal effect. The main role is associated with the effect of high temperature, which, in the form of flow in a liquid medium, affects bacteria.

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“…Incidentally, it has been shown in the aforementioned work (19) that laser light can partially compensate for the negative impact of the attendant electromagnetic field on Daphnia. On the other hand, it is known that the influence of low-intensity laser irradiation was most pronounced in weakened or nonhealthy cells (32)(33)(34).…”

Section: Introductionmentioning

confidence: 99%

Effects of Photobiomodulation on Daphnia magna Straus and their Sensitivity to Toxicant

Vorobyeva

Samoylova

Yusupov

2020

Photochem & Photobiology

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This paper deals with the effect of photobiomodulation (PBM) on Daphnia magna S. and their sensitivity to cadmium sulfate, a known high toxic pollutant. In a first series of experiments, the effect of different He‐Ne laser fluences irradiation (range 0.9‐4300 mJ cm−2) on the fertility of both parent and filial generations (F1–F3) of the crustacean was studied. It was found that PBM in some cases significantly influenced the fertility of both irradiated crustaceans and their nonirradiated offspring. By selecting two fluences (9 ± 2 mJ cm−2 reducing fertility and 4.3 ± 0.9 J cm−2 increasing it), the effect of these on toxicity of cadmium sulfate was evaluated. These experiments have shown that prior irradiation with low‐intensity light of a helium–neon laser with 632.8 nm wavelength can change the sensitivity of aquatic organisms to toxin cadmium sulfate. The degree and direction of changes depend on the toxicant concentration and the irradiation dose.

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Activation of Bone Marrow Multipotent Stromal Cells by Laser and EHF Radiation and Their Combined Impacts

Cited by 9 publications

References 5 publications

Effects of Acoustic and EHF Impulses on Multipotent Stromal Cells during Formation of Bone Marrow Containing Heterotopic Organs in Tissue Engineered Constructions

Effects of Acoustic and EHF Impulses on Multipotent Stromal Cells during Formation of Bone Marrow Containing Heterotopic Organs in Tissue Engineered Constructions

The bactericidal effect of continuous wave laser with strongly absorbing coating at the fiber tip

Effects of Photobiomodulation on Daphnia magna Straus and their Sensitivity to Toxicant

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