Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1–AtMEK1 pathway by wounding

Hadiarto, Toto; Nanmori, Takashi; Matsuoka, Daisuke; Iwasaki, Tetsushi; Sato, Kenichi; Fukami, Yasuo; Azuma, Tetsushi; Yasuda, Takeshi

doi:10.1007/s00425-005-0126-7

Cited by 51 publications

(42 citation statements)

References 15 publications

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“…Fifteen minutes after treatment, all three phosphorylated MAPK reactive signals on the western blot became stronger, comparably across all three genotypes. Therefore, unlike MEKK1D166 (Hadiarto et al, 2006), enzyme regulation and substrate preference do not appear to be altered in mekk1-5.…”

Section: Mekk1-5 Is a Partial Loss-of-function Mutant In Mekk1mentioning

confidence: 93%

“…The mekk1-5 mutation is within the first 166 amino acids of MEKK1, a region that when deleted (MEKK1D166) results in constitutive kinase activity in a protoplast system (Hadiarto et al, 2006) and has been implicated in the control of substrate specificity of this kinase (Ichimura et al, 2006;Suarez-Rodriguez et al, 2007). The proposed function of this region raised the possibility of altered regulation and substrate specificity/preference of MEKK1 in mekk1-5.…”

Section: Mekk1-5 Is a Partial Loss-of-function Mutant In Mekk1mentioning

confidence: 99%

“…A likely GSR regulatory candidate, known to be involved in multiple rapid stress signaling networks across kingdoms, is MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) signaling cascades, which consist of three levels of activating phosphorylation (MAPKKK, MAPKK, and MAPK; Ichimura et al, 1998;Zhang and Klessig, 2001;Nakagami et al, 2005;Andreasson and Ellis, 2010;Rodriguez et al, 2010;Šamajová et al, 2013). Within MAPK signaling cascades, of particular note is MEKK1, a MAPKKK implicated in myriad stress responses through multiple downstream signaling partners (Asai et al, 2002;Nakagami et al, 2005;Hadiarto et al, 2006). For example, in response to wounding, MEKK1 is necessary for MAPK4 activation through MEK1 and MEK2 (Ichimura et al, 2006).…”

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confidence: 99%

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Distinct Roles for Mitogen-Activated Protein Kinase Signaling and CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 in Regulating the Peak Time and Amplitude of the Plant General Stress Response

et al. 2014

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To survive environmental challenges, plants have evolved tightly regulated response networks, including a rapid and transient general stress response (GSR), followed by well-studied stress-specific responses. The mechanisms underpinning the GSR have remained elusive, but a functional cis-element, the rapid stress response element (RSRE), is known to confer transcription of GSR genes rapidly (5 min) and transiently (peaking 90-120 min after stress) in vivo. To investigate signal transduction events in the GSR, we used a 4xRSRE:LUCIFERASE reporter in Arabidopsis (Arabidopsis thaliana), employing complementary approaches of forward and chemical genetic screens, and identified components regulating peak time versus amplitude of RSRE activity. Specifically, we identified a mutant in CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 (CAMTA3) with reduced RSRE activation, verifying this transcription factor's role in activation of the RSRE-mediated GSR. Furthermore, we isolated a mutant in MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) KINASE KINASE1 (mekk1-5), which displays increased basal and an approximately 60-min earlier peak of wound-induced RSRE activation. The double mekk1/camta3 mutant positioned CAMTA3 downstream of MEKK1 and verified their distinct roles in GSR regulation. mekk1-5 displays programmed cell death and overaccumulates reactive oxygen species and salicylic acid, hallmarks of the hypersensitive response, suggesting that the hypersensitive response may play a role in the RSRE phenotype in this mutant. In addition, chemical inhibition studies suggest that the MAPK network is required for the rapid peak of the RSRE response, distinguishing the impact of chronic (mekk1-5) from transient (chemical inhibition) loss of MAPK signaling. Collectively, these results reveal underlying regulatory components of the plant GSR and further define their distinct roles in the regulation of this key biological process.

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Section: Mekk1-5 Is a Partial Loss-of-function Mutant In Mekk1mentioning

confidence: 93%

Section: Mekk1-5 Is a Partial Loss-of-function Mutant In Mekk1mentioning

confidence: 99%

mentioning

confidence: 99%

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Distinct Roles for Mitogen-Activated Protein Kinase Signaling and CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 in Regulating the Peak Time and Amplitude of the Plant General Stress Response

et al. 2014

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“…It has been previously reported that the MEKK1-MKK1-MPK4 cascade is stimulated following wounding stress (Hadiarto et al 2006;Matsuoka et al 2002). Other studies have also indicated that these cascades are involved in several other stress signalling pathways: the MEKK1-MKK2-MPK4/MPK6 cascade in salt and cold stress signalling (Teige et al 2004) or the MEKK1-MKK4/MKK5-MPK3/MPK6 cascade following pathogen infection (Asai et al 2002).…”

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confidence: 97%

Over-expression of MAP3K^|^#948;4, an ABA-inducible Raf-like MAP3K that confers salt tolerance in Arabidopsis

Shitamichi

Matsuoka

Sasayama

et al. 2013

Plant Biotechnology

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Mitogen-activated protein kinase (MAPK) cascades play important roles in plant responses to various environmental stimuli, including high salt or drought levels. Arabidopsis MAP3Kδ4 with PAS (period circadian protein, arylhydrocarbon receptor nuclear translocator protein and single-minded protein) domain is one of the Raftype MAPKKKs whose function has not been identified to date. Previous studies have shown that the MAP3Kδ4 overexpressing transformant exhibits vigorous growth. In this study, RT-PCR analysis showed that MAP3Kδ4 transcripts are increased through stress treatments, such as high salt, osmosis, drought and cold and the plant hormone, abscisic acid (ABA). The precipitation of MAP3Kδ4 using its specific antibody showed that ABA treatment markedly induces the activity of this enzyme. Furthermore, the ABA-mediated inhibition of seed germination was relieved in transgenic Arabidopsis over-expressing MAP3Kδ4. These results suggested that MAP3Kδ4 plays an important role in ABA signalling. Transgenic Arabidopsis over-expressing MAP3Kδ4 also exhibited enhanced tolerance to salt stress. The results obtained in this study demonstrated that MAP3Kδ4 was active during ABA-related responses and is involved in both stress tolerance and increased biomass. Therefore, MAP3Kδ4 and its counterpart genes are important with respect to agricultural developments.

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“…Yeast two-hybrid analyses indicated that MPK4, MKK1, and MKK2 also interact with the stressinduced MPKKK, MEKK1 Mizoguchi et al, 1998), and MEKK1 can activate both MKK1 (Hadiarto et al, 2006) and MKK2 (Teige et al, 2004). Mutations in MEKK1 (Ichimura et al, 2006;Nakagami et al, 2006;Suarez-Rodriguez et al, 2007) result in plants morphologically similar to mpk4 mutants, including dwarfed growth, enhanced PR1 gene expression, and reduced MPK4 activity induction by hydrogen peroxide or flagellin.…”

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confidence: 99%

Arabidopsis Mitogen-Activated Protein Kinase Kinases MKK1 and MKK2 Have Overlapping Functions in Defense Signaling Mediated by MEKK1, MPK4, and MKS1

et al. 2008

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The Arabidopsis (Arabidopsis thaliana) MKK1 and MKK2 mitogen-activated protein kinase kinases have been implicated in biotic and abiotic stress responses as part of a signaling cascade including MEKK1 and MPK4. Here, the double loss-offunction mutant (mkk1/2) of MKK1 and MKK2 is shown to have marked phenotypes in development and disease resistance similar to those of the single mekk1 and mpk4 mutants. Because mkk1 or mkk2 single mutants appear wild type, basal levels of MPK4 activity are not impaired in them, and MKK1 and MKK2 are in part functionally redundant in unchallenged plants. These findings are confirmed and extended by biochemical and molecular analyses implicating the kinases in jasmonate-and salicylate-dependent defense responses, mediated in part via the MPK4 substrate MKS1. In addition, transcriptome analyses delineate overlapping and specific effects of the kinases on global gene expression patterns demonstrating both redundant and unique functions for MKK1 and MKK2.

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Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1–AtMEK1 pathway by wounding

Cited by 51 publications

References 15 publications

Distinct Roles for Mitogen-Activated Protein Kinase Signaling and CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 in Regulating the Peak Time and Amplitude of the Plant General Stress Response

Distinct Roles for Mitogen-Activated Protein Kinase Signaling and CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 in Regulating the Peak Time and Amplitude of the Plant General Stress Response

Over-expression of MAP3K^|^#948;4, an ABA-inducible Raf-like MAP3K that confers salt tolerance in Arabidopsis

Arabidopsis Mitogen-Activated Protein Kinase Kinases MKK1 and MKK2 Have Overlapping Functions in Defense Signaling Mediated by MEKK1, MPK4, and MKS1

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