Activation of anaerobic metabolism in Biomphalaria glabrata (Mollusca: Gastropoda) experimentally infected by Angiostrongylus cantonensis (Nematoda, Metastrongylidae) by high-performance liquid chromatography

Tunholi-Alves, Vinícius Menezes; Tunholi, Victor Menezes; Castro, Rosane Nora; Sant’Ana, Luiza D‘Oliveira; Santos-Amaral, Luciana; Oliveira, Ana Paula Martins de; Garcia, Juberlan Silva; Thiengo, Silvana Carvalho; Pinheiro, Jairo; Maldonado, Arnaldo

doi:10.1016/j.parint.2013.09.004

Cited by 28 publications

(15 citation statements)

References 18 publications

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“…In Parmarion martensi semi-slugs (semi-slugs are molluscs with a shell that is too small to retract into), the midsection and tail had the highest larval densities; few larvae were found in the head (Jarvi et al 2012). Angiostrongylus cantonensis is also pathogenic to molluscs, inducing biochemical disturbances, inflammation and reduction in reproductive capacity (Yousif et al 1980; Tunholi-Alves et al 2011, 2012, 2014, 2015).…”

Section: Life Cycle and Transmissionmentioning

confidence: 99%

Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen

et al. 2016

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Angiostrongylus cantonensis is a metastrongyloid nematode found widely in the Asia-Pacific region, and the aetiological agent of angiostrongyliasis; a disease characterized by eosinophilic meningitis. Rattus rats are definitive hosts of A. cantonensis, while intermediate hosts include terrestrial and aquatic molluscs. Humans are dead-end hosts that usually become infected upon ingestion of infected molluscs. A presumptive diagnosis is often made based on clinical features, a history of mollusc consumption, eosinophilic pleocytosis in cerebral spinal fluid, and advanced imaging such as computed tomography. Serological tests are available for angiostrongyliasis, though many tests are still under development. While there is no treatment consensus, therapy often includes a combination of anthelmintics and corticosteroids. Angiostrongyliasis is relatively rare, but is often associated with morbidity and sometimes mortality. Recent reports suggest the parasites' range is increasing, leading to fatalities in regions previously considered Angiostrongylus-free, and sometimes, delayed diagnosis in newly invaded regions. Increased awareness of angiostrongyliasis would facilitate rapid diagnosis and improved clinical outcomes. This paper summarizes knowledge on the parasites' life cycle, clinical aspects and epidemiology. The molecular biology of Angiostrongylus spp. is also discussed. Attention is paid to the significance of angiostrongyliasis in Australia, given the recent severe cases reported from the Sydney region.

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Section: Life Cycle and Transmissionmentioning

confidence: 99%

Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen

et al. 2016

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“…The immortalization of Bge cells could also have occurred as a result of similar changes. Furthermore, it has been shown that infection of B. glabrata by various trematodes including S. mansoni [45–47], E. caproni [48], and E. paraensei [49] and the nematode Angiostrongylus cantonensis [50] greatly modifies the snail’s glucose metabolism and storage pathways. B. glabrata glycogen reserves decrease after A. cantonensi s and S. mansoni infection, and a significant reduction in the concentration of free glucose in the hemolymph is also a consequence of larval infections.…”

Section: Discussionmentioning

confidence: 99%

Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

et al. 2018

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BackgroundThe aquatic pulmonate snail Biomphalaria glabrata is a significant vector and laboratory host for the parasitic flatworm Schistosoma mansoni, an etiological agent for the neglected tropical disease schistosomiasis. Much is known regarding the host-parasite interactions of these two organisms, and the B. glabrata embryonic (Bge) cell line has been an invaluable resource in these studies. The B. glabrata BB02 genome sequence was recently released, but nothing is known of the sequence variation between this reference and the Bge cell genome, which has likely accumulated substantial genetic variation in the ~50 years since its isolation.ResultsHere, we report the genome sequence of our laboratory subculture of the Bge cell line (designated Bge3), which we mapped to the B. glabrata BB02 reference genome. Single nucleotide variants (SNVs) were predicted and focus was given to those SNVs that are most likely to affect the structure or expression of protein-coding genes. Furthermore, we have highlighted and validated high-impact SNVs in genes that have often been studied using Bge cells as an in vitro model, and other genes that may have contributed to the immortalization of this cell line. We also resolved representative karyotypes for the Bge3 subculture, which revealed a mixed population exhibiting substantial aneuploidy, in line with previous reports from other Bge subcultures.ConclusionsThe Bge3 genome differs from the B. glabrata BB02 reference genome in both sequence and structure, and these are likely to have significant biological effects. The availability of the Bge3 genome sequence, and an awareness of genomic differences with B. glabrata, will inform the design of experiments to understand gene function in this unique in vitro snail cell model. Additionally, this resource will aid in the development of new technologies and molecular approaches that promise to reveal more about this schistosomiasis-transmitting snail vector.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3059-2) contains supplementary material, which is available to authorized users.

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“…Recognitionally, the vertebrate host of A. cantonensis is a terrestrial rodent, which leads us to infer that the life cycle of this parasite would be more likely to be completed if the invertebrate intermediate host was a terrestrial mollusk than if it were an aquatic mollusk. Despite the habits of the definitive vertebrate host, rodents, the greatest contribution in the literature about the relationship between snail host and larval A. cantonensis was made in studies using a freshwater snail species, mainly B. glabrata a freshwater snail species that was never found naturally infected with A. cantonensis (Tunholi-Alves et al, 2014a, 2014b. Puthiyakunnon and Chen (2015) Why does this matter?…”

Section: Discussionmentioning

confidence: 99%

First record of Bulimulus tenuissimus (Mollusca) as potential experimental intermediate host of Angiostrongylus cantonensis (Nematoda)

et al. 2019

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Snails are essential to complete the life cycle of the metastrongylid nematode Angiostrongylus cantonensis, the causative agent of infections in domestic and wild animals, mainly rodents, and also of neural angiostrongyliasis or eosinophilic meningitis in humans. There are many reports of mollusks that can act as intermediate hosts of this parasite, especially freshwater snails and the African giant Achatina fulica. The terrestrial gastropod Bulimulus tenuissimus is widely distributed in Brazil and other species of the same genus occur in Brazil and other countries, overlapping regions in which there are reports of the occurrence of A. cantonensis and angiostrongyliasis. In spite of this, there are no records in the literature of this species performing the role of intermediate host to A. cantonensis. The present study analyzed the experimental infection with first-stage larvae of A. cantonensis, under laboratory conditions, of B. tenuissimus, by using histology and electron microscopy techniques. Three weeks after exposure to L1 larvae, it was possible to recover L3 larvae in small numbers from the infected snails. Developing larvae were observed in the cephalopedal mass (foot), ovotestis, and mantle tissues, being located inside a granulomatous structure composed of hemocyte infiltration, but there was no calcium or collagen deposition in these structures in significant amounts. In the third week post exposure, it was possible observe a sheath around the developing larvae. The infected snails presented reduction in the fibrous muscular tissue in the foot region, loss of the acinar organization in the digestive gland, with increase of amorphous material inside the acini and loss of epithelial pattern of nuclear organization in the acinar cells. However, the ovotestis seemed unaffected by the infection, since there was a large number of developing oocytes and spermatozoa in different stages of formation. The digestion of infected snails allows us the third-stage recovery rate of 17.25%, at 14 days post exposure to the L1. These L3 recovered from B. tenuissimus were used to infect rats experimentally, and 43 days post infection first-stage (L1) larvae of A. cantonensis were recovered from fresh feces. The results presented constituted the first report of the role of B. tenuissimus as an experimental intermediate host to A. cantonensis and shed some light on a possible problem, since the overlapping distribution of B. tenuissimus and A. cantonensis in Brazil and other countries where different species of Bulimulus occur enables the establishment and maintenance of the life cycle of this parasite in nature, with wild rodents as reservoirs, acting as a source of infection to humans, causing neural angiostrongyliasis.

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Activation of anaerobic metabolism in Biomphalaria glabrata (Mollusca: Gastropoda) experimentally infected by Angiostrongylus cantonensis (Nematoda, Metastrongylidae) by high-performance liquid chromatography

Cited by 28 publications

References 18 publications

Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen

Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen

Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

First record of Bulimulus tenuissimus (Mollusca) as potential experimental intermediate host of Angiostrongylus cantonensis (Nematoda)

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