Activation of Amino Acids in the Aorta

Abstract

Activation of 22 protein amino acids in the presence of enzyme preparation from pig aortas was shown. Detailed parameters (pH, reaction time and velocity, substrate and enzyme concentrations) of the reaction measured by two methods, the formation of amino acid hydroxamates and the amino acid-dependent exchange of PPi with ATP, were established.

Effect of Staphylococcal α-Toxin and Different Proteins on Amino Acid Activation in the Aorta

“…The means are similar to those obtained previously [9]. The appropriate variation coefficients, 5.8 and 10.2%, are insignificantly different from each other, as indicated by comparison of variances.…”

“…The initial step in protein synthesis, i.e. the activation of amino acids in the aorta has been investigated [3,9].…”

“…Acetone powders of thoracic aortas from pigs were processed to obtain enzyme preparations as described previously [9]. Protein was determined by the biuret method after Kingsley [6].…”

Activity of sRNA Amino Acyl Synthetases in Ontogenic Development of Rabbit Brain

“…Our results also agree with the previously observed trend to change the synthetase activity in the maturing central nervous system. By using a more sensitive and precise ATP-PP; exchange method [Patelski et al, 1973] statistically significant differences were shown between the activity of amino acyl sRNA synthetases in the grey and white matter. This proves their metabolic changeability in relation to the preliminary step of protein biosynthesis.…”

“…mg-1 of protein under the assay conditions above. For more details on methodology and kinetics of these reaction see Patelski et al [1973].…”